Establishment of reference CD4+ T cell values for adult Indian population
<p>Abstract</p> <p>Background</p> <p>CD4+ T lymphocyte counts are the most important indicator of disease progression and success of antiretroviral treatment in HIV infection in resource limited settings. The nationwide reference range of CD4+ T lymphocytes was not avai...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-10-01
|
| Series: | AIDS Research and Therapy |
| Online Access: | http://www.aidsrestherapy.com/content/8/1/35 |
| _version_ | 1818187482565443584 |
|---|---|
| author | Ray Krishnangshu Narain Kanwar Mohanakrishnan Janardhanan Desai Anita Vajpayee Madhu Vasanthapuram Ravi Devi K Rekha Joshi Ameeta A Bandyopadhyay Bhaswati Balakrishnan Pachamuthu Arora Sunil Abraham Philip R Thakar Madhuri R Patil Shilpa S Singh Ravinder Singla Anuj Paranjape Ramesh S |
| author_facet | Ray Krishnangshu Narain Kanwar Mohanakrishnan Janardhanan Desai Anita Vajpayee Madhu Vasanthapuram Ravi Devi K Rekha Joshi Ameeta A Bandyopadhyay Bhaswati Balakrishnan Pachamuthu Arora Sunil Abraham Philip R Thakar Madhuri R Patil Shilpa S Singh Ravinder Singla Anuj Paranjape Ramesh S |
| author_sort | Ray Krishnangshu |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>CD4+ T lymphocyte counts are the most important indicator of disease progression and success of antiretroviral treatment in HIV infection in resource limited settings. The nationwide reference range of CD4+ T lymphocytes was not available in India. This study was conducted to determine reference values of absolute CD4+ T cell counts and percentages for adult Indian population.</p> <p>Methods</p> <p>A multicentric study was conducted involving eight sites across the country. A total of 1206 (approximately 150 per/centre) healthy participants were enrolled in the study. The ratio of male (N = 645) to female (N = 561) of 1.14:1. The healthy status of the participants was assessed by a pre-decided questionnaire. At all centers the CD4+ T cell count, percentages and absolute CD3+ T cell count and percentages were estimated using a single platform strategy and lyse no wash technique. The data was analyzed using the Statistical Package for the Social Scientist (SPSS), version 15) and Prism software version 5.</p> <p>Results</p> <p>The absolute CD4+ T cell counts and percentages in female participants were significantly higher than the values obtained in male participants indicating the true difference in the CD4+ T cell subsets. The reference range for absolute CD4 count for Indian male population was 381-1565 cells/μL and for female population was 447-1846 cells/μL. The reference range for CD4% was 25-49% for male and 27-54% for female population. The reference values for CD3 counts were 776-2785 cells/μL for Indian male population and 826-2997 cells/μL for female population.</p> <p>Conclusion</p> <p>The study used stringent procedures for controlling the technical variation in the CD4 counts across the sites and thus could establish the robust national reference ranges for CD4 counts and percentages. These ranges will be helpful in staging the disease progression and monitoring antiretroviral therapy in HIV infection in India.</p> |
| first_indexed | 2024-12-11T23:11:44Z |
| format | Article |
| id | doaj.art-589afc43de9e4f9fb3436de644b31445 |
| institution | Directory Open Access Journal |
| issn | 1742-6405 |
| language | English |
| last_indexed | 2024-12-11T23:11:44Z |
| publishDate | 2011-10-01 |
| publisher | BMC |
| record_format | Article |
| series | AIDS Research and Therapy |
| spelling | doaj.art-589afc43de9e4f9fb3436de644b314452022-12-22T00:46:40ZengBMCAIDS Research and Therapy1742-64052011-10-01813510.1186/1742-6405-8-35Establishment of reference CD4+ T cell values for adult Indian populationRay KrishnangshuNarain KanwarMohanakrishnan JanardhananDesai AnitaVajpayee MadhuVasanthapuram RaviDevi K RekhaJoshi Ameeta ABandyopadhyay BhaswatiBalakrishnan PachamuthuArora SunilAbraham Philip RThakar Madhuri RPatil Shilpa SSingh RavinderSingla AnujParanjape Ramesh S<p>Abstract</p> <p>Background</p> <p>CD4+ T lymphocyte counts are the most important indicator of disease progression and success of antiretroviral treatment in HIV infection in resource limited settings. The nationwide reference range of CD4+ T lymphocytes was not available in India. This study was conducted to determine reference values of absolute CD4+ T cell counts and percentages for adult Indian population.</p> <p>Methods</p> <p>A multicentric study was conducted involving eight sites across the country. A total of 1206 (approximately 150 per/centre) healthy participants were enrolled in the study. The ratio of male (N = 645) to female (N = 561) of 1.14:1. The healthy status of the participants was assessed by a pre-decided questionnaire. At all centers the CD4+ T cell count, percentages and absolute CD3+ T cell count and percentages were estimated using a single platform strategy and lyse no wash technique. The data was analyzed using the Statistical Package for the Social Scientist (SPSS), version 15) and Prism software version 5.</p> <p>Results</p> <p>The absolute CD4+ T cell counts and percentages in female participants were significantly higher than the values obtained in male participants indicating the true difference in the CD4+ T cell subsets. The reference range for absolute CD4 count for Indian male population was 381-1565 cells/μL and for female population was 447-1846 cells/μL. The reference range for CD4% was 25-49% for male and 27-54% for female population. The reference values for CD3 counts were 776-2785 cells/μL for Indian male population and 826-2997 cells/μL for female population.</p> <p>Conclusion</p> <p>The study used stringent procedures for controlling the technical variation in the CD4 counts across the sites and thus could establish the robust national reference ranges for CD4 counts and percentages. These ranges will be helpful in staging the disease progression and monitoring antiretroviral therapy in HIV infection in India.</p>http://www.aidsrestherapy.com/content/8/1/35 |
| spellingShingle | Ray Krishnangshu Narain Kanwar Mohanakrishnan Janardhanan Desai Anita Vajpayee Madhu Vasanthapuram Ravi Devi K Rekha Joshi Ameeta A Bandyopadhyay Bhaswati Balakrishnan Pachamuthu Arora Sunil Abraham Philip R Thakar Madhuri R Patil Shilpa S Singh Ravinder Singla Anuj Paranjape Ramesh S Establishment of reference CD4+ T cell values for adult Indian population AIDS Research and Therapy |
| title | Establishment of reference CD4+ T cell values for adult Indian population |
| title_full | Establishment of reference CD4+ T cell values for adult Indian population |
| title_fullStr | Establishment of reference CD4+ T cell values for adult Indian population |
| title_full_unstemmed | Establishment of reference CD4+ T cell values for adult Indian population |
| title_short | Establishment of reference CD4+ T cell values for adult Indian population |
| title_sort | establishment of reference cd4 t cell values for adult indian population |
| url | http://www.aidsrestherapy.com/content/8/1/35 |
| work_keys_str_mv | AT raykrishnangshu establishmentofreferencecd4tcellvaluesforadultindianpopulation AT narainkanwar establishmentofreferencecd4tcellvaluesforadultindianpopulation AT mohanakrishnanjanardhanan establishmentofreferencecd4tcellvaluesforadultindianpopulation AT desaianita establishmentofreferencecd4tcellvaluesforadultindianpopulation AT vajpayeemadhu establishmentofreferencecd4tcellvaluesforadultindianpopulation AT vasanthapuramravi establishmentofreferencecd4tcellvaluesforadultindianpopulation AT devikrekha establishmentofreferencecd4tcellvaluesforadultindianpopulation AT joshiameetaa establishmentofreferencecd4tcellvaluesforadultindianpopulation AT bandyopadhyaybhaswati establishmentofreferencecd4tcellvaluesforadultindianpopulation AT balakrishnanpachamuthu establishmentofreferencecd4tcellvaluesforadultindianpopulation AT arorasunil establishmentofreferencecd4tcellvaluesforadultindianpopulation AT abrahamphilipr establishmentofreferencecd4tcellvaluesforadultindianpopulation AT thakarmadhurir establishmentofreferencecd4tcellvaluesforadultindianpopulation AT patilshilpas establishmentofreferencecd4tcellvaluesforadultindianpopulation AT singhravinder establishmentofreferencecd4tcellvaluesforadultindianpopulation AT singlaanuj establishmentofreferencecd4tcellvaluesforadultindianpopulation AT paranjaperameshs establishmentofreferencecd4tcellvaluesforadultindianpopulation |