Correlation of Metabolic Syndrome with Redox Homeostasis Biomarkers: Evidence from High-Fat Diet Model in Wistar Rats
Metabolic Syndrome (MetS) is an extremely complex disease. A non-balanced diet such as high-fat diet (HFD) induces metabolic dysfunction that could modify redox homeostasis. We here aimed at exploring redox homeostasis in male Wistar rats, following 8 weeks of HFD, correlating the eventual modificat...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/12/1/89 |
| _version_ | 1797446821889441792 |
|---|---|
| author | Danila Di Majo Pierangelo Sardo Giuseppe Giglia Valentina Di Liberto Francesco Paolo Zummo Maria Grazia Zizzo Gaetano Felice Caldara Francesca Rappa Giorgia Intili Roelof Maarten van Dijk Daniele Gallo Giuseppe Ferraro Giuditta Gambino |
| author_facet | Danila Di Majo Pierangelo Sardo Giuseppe Giglia Valentina Di Liberto Francesco Paolo Zummo Maria Grazia Zizzo Gaetano Felice Caldara Francesca Rappa Giorgia Intili Roelof Maarten van Dijk Daniele Gallo Giuseppe Ferraro Giuditta Gambino |
| author_sort | Danila Di Majo |
| collection | DOAJ |
| description | Metabolic Syndrome (MetS) is an extremely complex disease. A non-balanced diet such as high-fat diet (HFD) induces metabolic dysfunction that could modify redox homeostasis. We here aimed at exploring redox homeostasis in male Wistar rats, following 8 weeks of HFD, correlating the eventual modification of selected biomarkers that could be associated with the clinical manifestations of MetS. Therefore, we selected parameters relative to both the glucose tolerance and lipid altered metabolism, but also oxidative pattern. We assessed some biomarkers of oxidative stress i.e., thiols balance, lipid peroxidation and antioxidant barriers, via the use of specific biochemical assays, individuating eventual cross correlation with parameters relative to MetS through a Principal Component Analysis (PCA). The present study shows that 8 weeks of HFD induce MetS in rats, altering glucose and lipid homeostasis and increasing visceral adipose tissue, but also impairing the physiological antioxidant responses that could not counteract the oxidative stress condition. Crucially, cross-correlation analysis suggested that the assessment of specific oxidative stress parameters reported here can provide information comparable to the more widely acquired biomarkers of Mets such as glucose tolerance. Lastly, hepatic steatosis in association with the oxidative stress condition was also highlighted by histological analysis. This research will elucidate the fundamental impact of these oxidative stress parameters on MetS induced in the HFD rat model, tracing paths for developing prevention approaches. |
| first_indexed | 2024-03-09T13:47:06Z |
| format | Article |
| id | doaj.art-589dbdb073d341ff84352c701672e189 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T13:47:06Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-589dbdb073d341ff84352c701672e1892023-11-30T20:58:10ZengMDPI AGAntioxidants2076-39212022-12-011218910.3390/antiox12010089Correlation of Metabolic Syndrome with Redox Homeostasis Biomarkers: Evidence from High-Fat Diet Model in Wistar RatsDanila Di Majo0Pierangelo Sardo1Giuseppe Giglia2Valentina Di Liberto3Francesco Paolo Zummo4Maria Grazia Zizzo5Gaetano Felice Caldara6Francesca Rappa7Giorgia Intili8Roelof Maarten van Dijk9Daniele Gallo10Giuseppe Ferraro11Giuditta Gambino12Department of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze, 90128 Palermo, ItalyUniversity of Palermo, 90100 Palermo, ItalyDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyStaburo GmbH, 81549 München, GermanyDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyDepartment of Biomedicine, Neuroscience and Advanced Diagnostics (BIND), University of Palermo, 90127 Palermo, ItalyMetabolic Syndrome (MetS) is an extremely complex disease. A non-balanced diet such as high-fat diet (HFD) induces metabolic dysfunction that could modify redox homeostasis. We here aimed at exploring redox homeostasis in male Wistar rats, following 8 weeks of HFD, correlating the eventual modification of selected biomarkers that could be associated with the clinical manifestations of MetS. Therefore, we selected parameters relative to both the glucose tolerance and lipid altered metabolism, but also oxidative pattern. We assessed some biomarkers of oxidative stress i.e., thiols balance, lipid peroxidation and antioxidant barriers, via the use of specific biochemical assays, individuating eventual cross correlation with parameters relative to MetS through a Principal Component Analysis (PCA). The present study shows that 8 weeks of HFD induce MetS in rats, altering glucose and lipid homeostasis and increasing visceral adipose tissue, but also impairing the physiological antioxidant responses that could not counteract the oxidative stress condition. Crucially, cross-correlation analysis suggested that the assessment of specific oxidative stress parameters reported here can provide information comparable to the more widely acquired biomarkers of Mets such as glucose tolerance. Lastly, hepatic steatosis in association with the oxidative stress condition was also highlighted by histological analysis. This research will elucidate the fundamental impact of these oxidative stress parameters on MetS induced in the HFD rat model, tracing paths for developing prevention approaches.https://www.mdpi.com/2076-3921/12/1/89oxidative stressanti-oxidant barriersglucose tolerancelipid metabolismnon-alcoholic fatty liver diseaseadipose tissue distribution |
| spellingShingle | Danila Di Majo Pierangelo Sardo Giuseppe Giglia Valentina Di Liberto Francesco Paolo Zummo Maria Grazia Zizzo Gaetano Felice Caldara Francesca Rappa Giorgia Intili Roelof Maarten van Dijk Daniele Gallo Giuseppe Ferraro Giuditta Gambino Correlation of Metabolic Syndrome with Redox Homeostasis Biomarkers: Evidence from High-Fat Diet Model in Wistar Rats Antioxidants oxidative stress anti-oxidant barriers glucose tolerance lipid metabolism non-alcoholic fatty liver disease adipose tissue distribution |
| title | Correlation of Metabolic Syndrome with Redox Homeostasis Biomarkers: Evidence from High-Fat Diet Model in Wistar Rats |
| title_full | Correlation of Metabolic Syndrome with Redox Homeostasis Biomarkers: Evidence from High-Fat Diet Model in Wistar Rats |
| title_fullStr | Correlation of Metabolic Syndrome with Redox Homeostasis Biomarkers: Evidence from High-Fat Diet Model in Wistar Rats |
| title_full_unstemmed | Correlation of Metabolic Syndrome with Redox Homeostasis Biomarkers: Evidence from High-Fat Diet Model in Wistar Rats |
| title_short | Correlation of Metabolic Syndrome with Redox Homeostasis Biomarkers: Evidence from High-Fat Diet Model in Wistar Rats |
| title_sort | correlation of metabolic syndrome with redox homeostasis biomarkers evidence from high fat diet model in wistar rats |
| topic | oxidative stress anti-oxidant barriers glucose tolerance lipid metabolism non-alcoholic fatty liver disease adipose tissue distribution |
| url | https://www.mdpi.com/2076-3921/12/1/89 |
| work_keys_str_mv | AT daniladimajo correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats AT pierangelosardo correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats AT giuseppegiglia correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats AT valentinadiliberto correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats AT francescopaolozummo correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats AT mariagraziazizzo correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats AT gaetanofelicecaldara correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats AT francescarappa correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats AT giorgiaintili correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats AT roelofmaartenvandijk correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats AT danielegallo correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats AT giuseppeferraro correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats AT giudittagambino correlationofmetabolicsyndromewithredoxhomeostasisbiomarkersevidencefromhighfatdietmodelinwistarrats |