Evaluation of cellobiose dehydrogenase and laccase containing culture fluids of Termitomyces sp. OE147 for degradation of Reactive blue 21
This study evaluates culture filtrate, rich in cellobiose dehydrogenase and laccases, of Termitomyces sp. OE 147, in decolouration and degradation of Reactive blue (RB) 21. About 35% decolouration was achieved at low volumes of the culture supernatant without addition of external redox mediators. An...
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| Format: | Article |
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Elsevier
2016-12-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X16300443 |
| _version_ | 1811327673522192384 |
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| author | Rishabh Gangwar Shafaq Rasool Saroj Mishra |
| author_facet | Rishabh Gangwar Shafaq Rasool Saroj Mishra |
| author_sort | Rishabh Gangwar |
| collection | DOAJ |
| description | This study evaluates culture filtrate, rich in cellobiose dehydrogenase and laccases, of Termitomyces sp. OE 147, in decolouration and degradation of Reactive blue (RB) 21. About 35% decolouration was achieved at low volumes of the culture supernatant without addition of external redox mediators. An optimized dye to culture fluid ratio (75 ppm: 0.1 ml) at a pH of 4–5 resulted in removal of colour by 60%. The degradation products of RB21 were analysed by Electron Spray Ionization-Mass Spectrometry and several small molecules (of m/z 106–199) were detected. These were concluded to be o-Xylene, 2,3-Dihydro-1H-isoindole, Isoindole-1,3-dione, 2,Benzenesulfonyl-ethanol, (4-Hydroxy-phenyl)-sulfamic acid, 2,3-Dihydro-1H-isoindole-5-sulfonic acid and proposed to result from joint action of cellobiose dehydrogenase, laccase, peroxidases and unidentified oxidoreductases present in the culture fluids. Based on the products formed and the known reactions of these enzymes, a degradation pathway was proposed for RB21. The culture fluid was also effective in decolouration (by about 50%) and detoxification (by ∼25%) of the combined effluent collected from a local mill indicating a treatment process that bypasses use of H2O2 and toxic mediators. |
| first_indexed | 2024-04-13T15:12:12Z |
| format | Article |
| id | doaj.art-589e4fe4360e47f187300019ffc3cb4a |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-13T15:12:12Z |
| publishDate | 2016-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-589e4fe4360e47f187300019ffc3cb4a2022-12-22T02:41:58ZengElsevierBiotechnology Reports2215-017X2016-12-0112C526110.1016/j.btre.2016.10.002Evaluation of cellobiose dehydrogenase and laccase containing culture fluids of Termitomyces sp. OE147 for degradation of Reactive blue 21Rishabh Gangwar0Shafaq Rasool1Saroj Mishra2Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi 110016, IndiaSchool of Biotechnology, Shri Mata Vaishno Devi University, Katra, Jammu and Kashmir 182320, IndiaDepartment of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, New Delhi 110016, IndiaThis study evaluates culture filtrate, rich in cellobiose dehydrogenase and laccases, of Termitomyces sp. OE 147, in decolouration and degradation of Reactive blue (RB) 21. About 35% decolouration was achieved at low volumes of the culture supernatant without addition of external redox mediators. An optimized dye to culture fluid ratio (75 ppm: 0.1 ml) at a pH of 4–5 resulted in removal of colour by 60%. The degradation products of RB21 were analysed by Electron Spray Ionization-Mass Spectrometry and several small molecules (of m/z 106–199) were detected. These were concluded to be o-Xylene, 2,3-Dihydro-1H-isoindole, Isoindole-1,3-dione, 2,Benzenesulfonyl-ethanol, (4-Hydroxy-phenyl)-sulfamic acid, 2,3-Dihydro-1H-isoindole-5-sulfonic acid and proposed to result from joint action of cellobiose dehydrogenase, laccase, peroxidases and unidentified oxidoreductases present in the culture fluids. Based on the products formed and the known reactions of these enzymes, a degradation pathway was proposed for RB21. The culture fluid was also effective in decolouration (by about 50%) and detoxification (by ∼25%) of the combined effluent collected from a local mill indicating a treatment process that bypasses use of H2O2 and toxic mediators.http://www.sciencedirect.com/science/article/pii/S2215017X16300443Termitomyces sp. OE 147Fungal culture filtratesComplex phthalocyanine dyesCellobiose dehydrogenase/laccaseTextile effluents |
| spellingShingle | Rishabh Gangwar Shafaq Rasool Saroj Mishra Evaluation of cellobiose dehydrogenase and laccase containing culture fluids of Termitomyces sp. OE147 for degradation of Reactive blue 21 Biotechnology Reports Termitomyces sp. OE 147 Fungal culture filtrates Complex phthalocyanine dyes Cellobiose dehydrogenase/laccase Textile effluents |
| title | Evaluation of cellobiose dehydrogenase and laccase containing culture fluids of Termitomyces sp. OE147 for degradation of Reactive blue 21 |
| title_full | Evaluation of cellobiose dehydrogenase and laccase containing culture fluids of Termitomyces sp. OE147 for degradation of Reactive blue 21 |
| title_fullStr | Evaluation of cellobiose dehydrogenase and laccase containing culture fluids of Termitomyces sp. OE147 for degradation of Reactive blue 21 |
| title_full_unstemmed | Evaluation of cellobiose dehydrogenase and laccase containing culture fluids of Termitomyces sp. OE147 for degradation of Reactive blue 21 |
| title_short | Evaluation of cellobiose dehydrogenase and laccase containing culture fluids of Termitomyces sp. OE147 for degradation of Reactive blue 21 |
| title_sort | evaluation of cellobiose dehydrogenase and laccase containing culture fluids of termitomyces sp oe147 for degradation of reactive blue 21 |
| topic | Termitomyces sp. OE 147 Fungal culture filtrates Complex phthalocyanine dyes Cellobiose dehydrogenase/laccase Textile effluents |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X16300443 |
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