Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae)
Abstract Bacillus thuringiensis (Bt) isolates native to Maranhão (BtMA) that are highly toxic to Aedes aegypti larvae and seven standard subspecies of Bt were analyzed for genetic diversity using the rep-PRC technique with BOX, ERIC, REP, MB1, and GTG5 markers. The rep-PCR technique is considered an...
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| Format: | Article |
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Nature Portfolio
2022-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-18559-0 |
| _version_ | 1811188799597707264 |
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| author | Geysla da Costa Fernandes Dalton Kaynnan de Prado Costa Nayanne Santos de Oliveira Emanuelle Cristine Pereira de Sousa Déborah Heloísa Bittencourt Machado Ricardo Antonio Polanczyk Herbert Álvaro Abreu de Siqueira Maria Cleoneide da Silva |
| author_facet | Geysla da Costa Fernandes Dalton Kaynnan de Prado Costa Nayanne Santos de Oliveira Emanuelle Cristine Pereira de Sousa Déborah Heloísa Bittencourt Machado Ricardo Antonio Polanczyk Herbert Álvaro Abreu de Siqueira Maria Cleoneide da Silva |
| author_sort | Geysla da Costa Fernandes |
| collection | DOAJ |
| description | Abstract Bacillus thuringiensis (Bt) isolates native to Maranhão (BtMA) that are highly toxic to Aedes aegypti larvae and seven standard subspecies of Bt were analyzed for genetic diversity using the rep-PRC technique with BOX, ERIC, REP, MB1, and GTG5 markers. The rep-PCR technique is considered an extremely reliable, reproducible, fast and highly discriminatory technique that may be used even among populations of the same species. These five markers revealed a total of 38 polymorphic DNA fragments for 30 BtMA isolates. Eight groups were obtained with the dendrogram generated through Pearson's correlation analysis, with four groups formed only with BtMA isolates and four comprised of isolates of BtMA and the standard subspecies toxic to dipterans and lepidopterans. Despite the high genetic diversity of BtMA, a low correlation between the collection site, gene content and mortality against A. aegypti larvae was evidenced. The clustering of the standard subspecies of Bt that were toxic against dipterans with BtMA isolates confirm the mosquitocidal action of the native isolates from Maranhão, and they can be used as an alternative for A. aegypti control and other insects of medical importance and for the control of agricultural pests. |
| first_indexed | 2024-04-11T14:25:26Z |
| format | Article |
| id | doaj.art-58a1545c70a44cd2afc646624609988d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T14:25:26Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-58a1545c70a44cd2afc646624609988d2022-12-22T04:18:53ZengNature PortfolioScientific Reports2045-23222022-08-011211910.1038/s41598-022-18559-0Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae)Geysla da Costa Fernandes0Dalton Kaynnan de Prado Costa1Nayanne Santos de Oliveira2Emanuelle Cristine Pereira de Sousa3Déborah Heloísa Bittencourt Machado4Ricardo Antonio Polanczyk5Herbert Álvaro Abreu de Siqueira6Maria Cleoneide da Silva7PostGraduate Program in Biodiversity, Environment and Health, Center for Higher Studies of Caxias (CESC), State University of Maranhão (UEMA)Laboratory of Entomopathogenic Bacteria and Molecular Markers, Center for Higher Studies of Caxias (CESC), State University of Maranhão (UEMA)Laboratory of Entomopathogenic Bacteria and Molecular Markers, Center for Higher Studies of Caxias (CESC), State University of Maranhão (UEMA)Postgraduate Program in Animal Science, State University of Maranhão (UEMA), University City Paul VIPostgraduate Program in Agronomy (Agricultural Entomology), São Paulo State University (UNESP), School of Agricultural and Veterinarian SciencesDepartment of Agricultural Production Sciences, São Paulo State University (UNESP), School of Agricultural and Veterinarian SciencesDepartment of Agronomy (Entomology), Federal Rural University of Pernambuco (UFRPE)Department of Chemistry and Biology, Laboratory of Entomopathogenic Bacteria and Molecular Markers, Center for Higher Studies of Caxias, State University of MaranhãoAbstract Bacillus thuringiensis (Bt) isolates native to Maranhão (BtMA) that are highly toxic to Aedes aegypti larvae and seven standard subspecies of Bt were analyzed for genetic diversity using the rep-PRC technique with BOX, ERIC, REP, MB1, and GTG5 markers. The rep-PCR technique is considered an extremely reliable, reproducible, fast and highly discriminatory technique that may be used even among populations of the same species. These five markers revealed a total of 38 polymorphic DNA fragments for 30 BtMA isolates. Eight groups were obtained with the dendrogram generated through Pearson's correlation analysis, with four groups formed only with BtMA isolates and four comprised of isolates of BtMA and the standard subspecies toxic to dipterans and lepidopterans. Despite the high genetic diversity of BtMA, a low correlation between the collection site, gene content and mortality against A. aegypti larvae was evidenced. The clustering of the standard subspecies of Bt that were toxic against dipterans with BtMA isolates confirm the mosquitocidal action of the native isolates from Maranhão, and they can be used as an alternative for A. aegypti control and other insects of medical importance and for the control of agricultural pests.https://doi.org/10.1038/s41598-022-18559-0 |
| spellingShingle | Geysla da Costa Fernandes Dalton Kaynnan de Prado Costa Nayanne Santos de Oliveira Emanuelle Cristine Pereira de Sousa Déborah Heloísa Bittencourt Machado Ricardo Antonio Polanczyk Herbert Álvaro Abreu de Siqueira Maria Cleoneide da Silva Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) Scientific Reports |
| title | Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) |
| title_full | Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) |
| title_fullStr | Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) |
| title_full_unstemmed | Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) |
| title_short | Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) |
| title_sort | genetic diversity of brazilian bacillus thuringiensis isolates with toxicity against aedes aegypti diptera culicidae |
| url | https://doi.org/10.1038/s41598-022-18559-0 |
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