Enzymatic spectrophotometric reaction rate determination of aspartame
Aspartame is an artificial sweetener of low caloric value (approximately 200 times sweeter than sucrose). Aspartame is currently permitted for use in food and beverage production in more than 90 countries. The application of aspartame in food products requires development of rapid, inexpens...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Association of Chemical Engineers of Serbia
2015-01-01
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| Series: | Hemijska Industrija |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0367-598X/2015/0367-598X1400049T.pdf |
| Summary: | Aspartame is an artificial sweetener of low caloric value (approximately 200
times sweeter than sucrose). Aspartame is currently permitted for use in
food and beverage production in more than 90 countries. The application of
aspartame in food products requires development of rapid, inexpensive and
accurate method for its determination. The new assay for determination of
aspartame was based on set of reactions that are catalyzed by three
different enzymes: α-chymotrypsin, alcohol oxidase and horseradish
peroxidase. Optimization of the proposed method was carried out for: (i)
α-chymotrypsin activity; (ii) time allowed for α-chymotrypsin action, (iii)
temperature. Evaluation of the developed method was done by determining
aspartame content in “diet” drinks, as well as in artificial sweetener
pills. [Projekat Ministarstva nauke Republike Srbije, br. III46010] |
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| ISSN: | 0367-598X 2217-7426 |