Validation of the T86I mutation in the gyrA gene as a highly reliable real time PCR target to detect Fluoroquinolone-resistant Campylobacter jejuni
Abstract Background Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide, and increasing rates of fluoroquinolone (FQ) resistance in C. jejuni are a major public health concern. The rapid detection and tracking of FQ resistance are critical needs in developing countries, as these...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12879-020-05202-4 |
| _version_ | 1818198810442072064 |
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| author | Nereyda Espinoza Jesús Rojas Simon Pollett Rina Meza Lilian Patiño Manuel Leiva Máximo Camiña Manuela Bernal Nathanael D. Reynolds Ryan Maves Drake H. Tilley Matthew Kasper Mark P. Simons |
| author_facet | Nereyda Espinoza Jesús Rojas Simon Pollett Rina Meza Lilian Patiño Manuel Leiva Máximo Camiña Manuela Bernal Nathanael D. Reynolds Ryan Maves Drake H. Tilley Matthew Kasper Mark P. Simons |
| author_sort | Nereyda Espinoza |
| collection | DOAJ |
| description | Abstract Background Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide, and increasing rates of fluoroquinolone (FQ) resistance in C. jejuni are a major public health concern. The rapid detection and tracking of FQ resistance are critical needs in developing countries, as these antimicrobials are widely used against C. jejuni infections. Detection of point mutations at T86I in the gyrA gene by real-time polymerase chain reaction (RT-PCR) is a rapid detection tool that may improve FQ resistance tracking. Methods C. jejuni isolates obtained from children with diarrhea in Peru were tested by RT-PCR to detect point mutations at T86I in gyrA. Further confirmation was performed by sequencing of the gyrA gene. Results We detected point mutations at T86I in the gyrA gene in 100% (141/141) of C. jejuni clinical isolates that were previously confirmed as ciprofloxacin-resistant by E-test. No mutations were detected at T86I in gyrA in any ciprofloxacin-sensitive isolates. Conclusions Detection of T86I mutations in C. jejuni is a rapid, sensitive, and specific method to identify fluoroquinolone resistance in Peru. This detection approach could be broadly employed in epidemiologic surveillance, therefore reducing time and cost in regions with limited resources. |
| first_indexed | 2024-12-12T02:11:47Z |
| format | Article |
| id | doaj.art-58a3a70641724983815cfb07a56abe43 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-12T02:11:47Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-58a3a70641724983815cfb07a56abe432022-12-22T00:41:53ZengBMCBMC Infectious Diseases1471-23342020-07-012011710.1186/s12879-020-05202-4Validation of the T86I mutation in the gyrA gene as a highly reliable real time PCR target to detect Fluoroquinolone-resistant Campylobacter jejuniNereyda Espinoza0Jesús Rojas1Simon Pollett2Rina Meza3Lilian Patiño4Manuel Leiva5Máximo Camiña6Manuela Bernal7Nathanael D. Reynolds8Ryan Maves9Drake H. Tilley10Matthew Kasper11Mark P. Simons12Bacteriology Department, U.S Naval Medical Research Unit-6 (NAMRU-6)Bacteriology Department, U.S Naval Medical Research Unit-6 (NAMRU-6)Bacteriology Department, U.S Naval Medical Research Unit-6 (NAMRU-6)Bacteriology Department, U.S Naval Medical Research Unit-6 (NAMRU-6)Instituto Nacional de Salud del NiñoHospital Nacional Docente Madre-Niño San BartoloméHospital de Emergencias PediátricasBacteriology Department, U.S Naval Medical Research Unit-6 (NAMRU-6)Naval Medical Research CenterNaval Medical Center San DiegoNaval Medical Center San DiegoBacteriology Department, U.S Naval Medical Research Unit-6 (NAMRU-6)Naval Medical Research CenterAbstract Background Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide, and increasing rates of fluoroquinolone (FQ) resistance in C. jejuni are a major public health concern. The rapid detection and tracking of FQ resistance are critical needs in developing countries, as these antimicrobials are widely used against C. jejuni infections. Detection of point mutations at T86I in the gyrA gene by real-time polymerase chain reaction (RT-PCR) is a rapid detection tool that may improve FQ resistance tracking. Methods C. jejuni isolates obtained from children with diarrhea in Peru were tested by RT-PCR to detect point mutations at T86I in gyrA. Further confirmation was performed by sequencing of the gyrA gene. Results We detected point mutations at T86I in the gyrA gene in 100% (141/141) of C. jejuni clinical isolates that were previously confirmed as ciprofloxacin-resistant by E-test. No mutations were detected at T86I in gyrA in any ciprofloxacin-sensitive isolates. Conclusions Detection of T86I mutations in C. jejuni is a rapid, sensitive, and specific method to identify fluoroquinolone resistance in Peru. This detection approach could be broadly employed in epidemiologic surveillance, therefore reducing time and cost in regions with limited resources.http://link.springer.com/article/10.1186/s12879-020-05202-4Campylobacter jejuniT86IgyrACiprofloxacinAntimicrobial resistance |
| spellingShingle | Nereyda Espinoza Jesús Rojas Simon Pollett Rina Meza Lilian Patiño Manuel Leiva Máximo Camiña Manuela Bernal Nathanael D. Reynolds Ryan Maves Drake H. Tilley Matthew Kasper Mark P. Simons Validation of the T86I mutation in the gyrA gene as a highly reliable real time PCR target to detect Fluoroquinolone-resistant Campylobacter jejuni BMC Infectious Diseases Campylobacter jejuni T86I gyrA Ciprofloxacin Antimicrobial resistance |
| title | Validation of the T86I mutation in the gyrA gene as a highly reliable real time PCR target to detect Fluoroquinolone-resistant Campylobacter jejuni |
| title_full | Validation of the T86I mutation in the gyrA gene as a highly reliable real time PCR target to detect Fluoroquinolone-resistant Campylobacter jejuni |
| title_fullStr | Validation of the T86I mutation in the gyrA gene as a highly reliable real time PCR target to detect Fluoroquinolone-resistant Campylobacter jejuni |
| title_full_unstemmed | Validation of the T86I mutation in the gyrA gene as a highly reliable real time PCR target to detect Fluoroquinolone-resistant Campylobacter jejuni |
| title_short | Validation of the T86I mutation in the gyrA gene as a highly reliable real time PCR target to detect Fluoroquinolone-resistant Campylobacter jejuni |
| title_sort | validation of the t86i mutation in the gyra gene as a highly reliable real time pcr target to detect fluoroquinolone resistant campylobacter jejuni |
| topic | Campylobacter jejuni T86I gyrA Ciprofloxacin Antimicrobial resistance |
| url | http://link.springer.com/article/10.1186/s12879-020-05202-4 |
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