Comparative transcriptome analysis of second- and third-generation merozoites of Eimeria necatrix
Abstract Background Eimeria is a common genus of apicomplexan parasites that infect diverse vertebrates, most notably poultry, causing serious disease and economic losses. Eimeria species have complex life-cycles consisting of three developmental stages. However, the molecular basis of the Eimeria r...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-017-2325-z |
| _version_ | 1818067773286252544 |
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| author | Shijie Su Zhaofeng Hou Dandan Liu Chuanli Jia Lele Wang Jinjun Xu Jianping Tao |
| author_facet | Shijie Su Zhaofeng Hou Dandan Liu Chuanli Jia Lele Wang Jinjun Xu Jianping Tao |
| author_sort | Shijie Su |
| collection | DOAJ |
| description | Abstract Background Eimeria is a common genus of apicomplexan parasites that infect diverse vertebrates, most notably poultry, causing serious disease and economic losses. Eimeria species have complex life-cycles consisting of three developmental stages. However, the molecular basis of the Eimeria reproductive mode switch remains an enigma. Methods Total RNA extracted from second- (MZ-2) and third-generation merozoites (MZ-3) of Eimeria necatrix was subjected to transcriptome analysis using RNA sequencing (RNA-seq) followed by qRT-PCR validation. Results A total of 6977 and 6901 unigenes were obtained from MZ-2 and MZ-3, respectively. Approximately 2053 genes were differentially expressed genes (DEGs) between MZ-2 and MZ-3. Compared with MZ-2, 837 genes were upregulated and 1216 genes were downregulated in MZ-3. Approximately 95 genes in MZ-2 and 48 genes in MZ-3 were further identified to have stage-specific expression. Gene ontology category and KEGG analysis suggested that 216 upregulated genes in MZ-2 were annotated by 70 GO assignments, 242 upregulated genes were associated with 188 signal pathways, while 321 upregulated genes in MZ-3 were annotated by 56 GO assignments, 322 upregulated genes were associated with 168 signal pathways. The molecular functions of upregulated genes in MZ-2 were mainly enriched for protein degradation and amino acid metabolism. The molecular functions of upregulated genes in MZ-3 were mainly enriched for transcriptional activity, cell proliferation and cell differentiation. Conclusions To the best of our knowledge, this is the first RNA-seq data study of the MZ-2 and MZ-3 stages of E. necatrix; it demonstrates a high number of differentially expressed genes between the MZ-2 and MZ-3 of E. necatrix. This study forms a basis for deciphering the molecular mechanisms underlying the shift from the second to third generation schizogony in Eimeria. It also provides valuable resources for future studies on Eimeria, and provides insight into the understanding of reproductive mode plasticity in different Eimeria species. |
| first_indexed | 2024-12-10T15:29:00Z |
| format | Article |
| id | doaj.art-58a9b92fff164018b840d6107944c54d |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-10T15:29:00Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-58a9b92fff164018b840d6107944c54d2022-12-22T01:43:28ZengBMCParasites & Vectors1756-33052017-08-0110111610.1186/s13071-017-2325-zComparative transcriptome analysis of second- and third-generation merozoites of Eimeria necatrixShijie Su0Zhaofeng Hou1Dandan Liu2Chuanli Jia3Lele Wang4Jinjun Xu5Jianping Tao6College of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityAbstract Background Eimeria is a common genus of apicomplexan parasites that infect diverse vertebrates, most notably poultry, causing serious disease and economic losses. Eimeria species have complex life-cycles consisting of three developmental stages. However, the molecular basis of the Eimeria reproductive mode switch remains an enigma. Methods Total RNA extracted from second- (MZ-2) and third-generation merozoites (MZ-3) of Eimeria necatrix was subjected to transcriptome analysis using RNA sequencing (RNA-seq) followed by qRT-PCR validation. Results A total of 6977 and 6901 unigenes were obtained from MZ-2 and MZ-3, respectively. Approximately 2053 genes were differentially expressed genes (DEGs) between MZ-2 and MZ-3. Compared with MZ-2, 837 genes were upregulated and 1216 genes were downregulated in MZ-3. Approximately 95 genes in MZ-2 and 48 genes in MZ-3 were further identified to have stage-specific expression. Gene ontology category and KEGG analysis suggested that 216 upregulated genes in MZ-2 were annotated by 70 GO assignments, 242 upregulated genes were associated with 188 signal pathways, while 321 upregulated genes in MZ-3 were annotated by 56 GO assignments, 322 upregulated genes were associated with 168 signal pathways. The molecular functions of upregulated genes in MZ-2 were mainly enriched for protein degradation and amino acid metabolism. The molecular functions of upregulated genes in MZ-3 were mainly enriched for transcriptional activity, cell proliferation and cell differentiation. Conclusions To the best of our knowledge, this is the first RNA-seq data study of the MZ-2 and MZ-3 stages of E. necatrix; it demonstrates a high number of differentially expressed genes between the MZ-2 and MZ-3 of E. necatrix. This study forms a basis for deciphering the molecular mechanisms underlying the shift from the second to third generation schizogony in Eimeria. It also provides valuable resources for future studies on Eimeria, and provides insight into the understanding of reproductive mode plasticity in different Eimeria species.http://link.springer.com/article/10.1186/s13071-017-2325-zEimeriaMerozoiteRNA-seqComparative transriptomesDifferentially expressed genes |
| spellingShingle | Shijie Su Zhaofeng Hou Dandan Liu Chuanli Jia Lele Wang Jinjun Xu Jianping Tao Comparative transcriptome analysis of second- and third-generation merozoites of Eimeria necatrix Parasites & Vectors Eimeria Merozoite RNA-seq Comparative transriptomes Differentially expressed genes |
| title | Comparative transcriptome analysis of second- and third-generation merozoites of Eimeria necatrix |
| title_full | Comparative transcriptome analysis of second- and third-generation merozoites of Eimeria necatrix |
| title_fullStr | Comparative transcriptome analysis of second- and third-generation merozoites of Eimeria necatrix |
| title_full_unstemmed | Comparative transcriptome analysis of second- and third-generation merozoites of Eimeria necatrix |
| title_short | Comparative transcriptome analysis of second- and third-generation merozoites of Eimeria necatrix |
| title_sort | comparative transcriptome analysis of second and third generation merozoites of eimeria necatrix |
| topic | Eimeria Merozoite RNA-seq Comparative transriptomes Differentially expressed genes |
| url | http://link.springer.com/article/10.1186/s13071-017-2325-z |
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