Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus
OBJECTIVES: The aim of this study was to compare the expression levels of serum miRNAs in diabetic retinopathy and type 2 diabetes mellitus. METHODS: Serum miRNA expression profiles from diabetic retinopathy cases (type 2 diabetes mellitus patients with diabetic retinopathy) and type 2 diabetes mel...
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| Format: | Article |
| Language: | English |
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Elsevier España
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| Series: | Clinics |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322017000200111&lng=en&tlng=en |
| _version_ | 1818245697835630592 |
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| author | Jianping Ma Jufang Wang Yanfen Liu Changyi Wang Donghui Duan Nanjia Lu Kaiyue Wang Lu Zhang Kaibo Gu Sihan Chen Tao Zhang Dingyun You Liyuan Han |
| author_facet | Jianping Ma Jufang Wang Yanfen Liu Changyi Wang Donghui Duan Nanjia Lu Kaiyue Wang Lu Zhang Kaibo Gu Sihan Chen Tao Zhang Dingyun You Liyuan Han |
| author_sort | Jianping Ma |
| collection | DOAJ |
| description | OBJECTIVES: The aim of this study was to compare the expression levels of serum miRNAs in diabetic retinopathy and type 2 diabetes mellitus. METHODS: Serum miRNA expression profiles from diabetic retinopathy cases (type 2 diabetes mellitus patients with diabetic retinopathy) and type 2 diabetes mellitus controls (type 2 diabetes mellitus patients without diabetic retinopathy) were examined by miRNA-specific microarray analysis. Quantitative real-time polymerase chain reaction was used to validate the significantly differentially expressed serum miRNAs from the microarray analysis of 45 diabetic retinopathy cases and 45 age-, sex-, body mass index- and duration-of-diabetes-matched type 2 diabetes mellitus controls. The relative changes in serum miRNA expression levels were analyzed using the 2-ΔΔCt method. RESULTS: A total of 5 diabetic retinopathy cases and 5 type 2 diabetes mellitus controls were included in the miRNA-specific microarray analysis. The serum levels of miR-3939 and miR-1910-3p differed significantly between the two groups in the screening stage; however, quantitative real-time polymerase chain reaction did not reveal significant differences in miRNA expression for 45 diabetic retinopathy cases and their matched type 2 diabetes mellitus controls. CONCLUSION: Our findings indicate that miR-3939 and miR-1910-3p may not play important roles in the development of diabetic retinopathy; however, studies with a larger sample size are needed to confirm our findings. |
| first_indexed | 2024-12-12T14:37:02Z |
| format | Article |
| id | doaj.art-58ac1f880557495eb9df255033f20f33 |
| institution | Directory Open Access Journal |
| issn | 1807-5932 1980-5322 |
| language | English |
| last_indexed | 2024-12-12T14:37:02Z |
| publisher | Elsevier España |
| record_format | Article |
| series | Clinics |
| spelling | doaj.art-58ac1f880557495eb9df255033f20f332022-12-22T00:21:21ZengElsevier EspañaClinics1807-59321980-532272211111510.6061/clinics/2017(02)08S1807-59322017000200111Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitusJianping MaJufang WangYanfen LiuChangyi WangDonghui DuanNanjia LuKaiyue WangLu ZhangKaibo GuSihan ChenTao ZhangDingyun YouLiyuan HanOBJECTIVES: The aim of this study was to compare the expression levels of serum miRNAs in diabetic retinopathy and type 2 diabetes mellitus. METHODS: Serum miRNA expression profiles from diabetic retinopathy cases (type 2 diabetes mellitus patients with diabetic retinopathy) and type 2 diabetes mellitus controls (type 2 diabetes mellitus patients without diabetic retinopathy) were examined by miRNA-specific microarray analysis. Quantitative real-time polymerase chain reaction was used to validate the significantly differentially expressed serum miRNAs from the microarray analysis of 45 diabetic retinopathy cases and 45 age-, sex-, body mass index- and duration-of-diabetes-matched type 2 diabetes mellitus controls. The relative changes in serum miRNA expression levels were analyzed using the 2-ΔΔCt method. RESULTS: A total of 5 diabetic retinopathy cases and 5 type 2 diabetes mellitus controls were included in the miRNA-specific microarray analysis. The serum levels of miR-3939 and miR-1910-3p differed significantly between the two groups in the screening stage; however, quantitative real-time polymerase chain reaction did not reveal significant differences in miRNA expression for 45 diabetic retinopathy cases and their matched type 2 diabetes mellitus controls. CONCLUSION: Our findings indicate that miR-3939 and miR-1910-3p may not play important roles in the development of diabetic retinopathy; however, studies with a larger sample size are needed to confirm our findings.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322017000200111&lng=en&tlng=entype 2 diabetes mellitusdiabetic retinopathyserum mirnas |
| spellingShingle | Jianping Ma Jufang Wang Yanfen Liu Changyi Wang Donghui Duan Nanjia Lu Kaiyue Wang Lu Zhang Kaibo Gu Sihan Chen Tao Zhang Dingyun You Liyuan Han Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus Clinics type 2 diabetes mellitus diabetic retinopathy serum mirnas |
| title | Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus |
| title_full | Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus |
| title_fullStr | Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus |
| title_full_unstemmed | Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus |
| title_short | Comparisons of serum miRNA expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus |
| title_sort | comparisons of serum mirna expression profiles in patients with diabetic retinopathy and type 2 diabetes mellitus |
| topic | type 2 diabetes mellitus diabetic retinopathy serum mirnas |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322017000200111&lng=en&tlng=en |
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