On-Site Diagnosis of Fire Blight with Antibody-Based Diagnostic Strips
Recently fire blight occurred in the Republic of Korea and eradication program for the disease has been executed since then. Specificity and detection sensitivity of the 2 antibody-based diagnostic strips to Korean isolates of Erwiniaamylovora (Ea) and their application for on-site diagnosis were ev...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2017-12-01
|
| Series: | Research in Plant Disease |
| Subjects: | |
| Online Access: | http://www.online-rpd.org/journal/view.html?doi=10.5423/RPD.2017.23.4.306 |
| _version_ | 1819275062353592320 |
|---|---|
| author | Gwang-Il Heo Doo-San Shin Soo-Hyeong Son Chang-Sik Oh Duck Hwan Park Young-Kee Lee Jae-Soon Cha |
| author_facet | Gwang-Il Heo Doo-San Shin Soo-Hyeong Son Chang-Sik Oh Duck Hwan Park Young-Kee Lee Jae-Soon Cha |
| author_sort | Gwang-Il Heo |
| collection | DOAJ |
| description | Recently fire blight occurred in the Republic of Korea and eradication program for the disease has been executed since then. Specificity and detection sensitivity of the 2 antibody-based diagnostic strips to Korean isolates of Erwiniaamylovora (Ea) and their application for on-site diagnosis were evaluated in this study. EaAgriStrip, a commercial diagnostic kit, and EB strip, developed in this study, reacted positively to the all tested Korean Ea strains and also to most of Erwiniapyrifoliae (Ep) strains causing black shoot blight. They reacted negatively to all Pusedomonassyringae pv. syringae (Pss) strains that cause shoot blight on apple. Detection sensitivity was similar between the 2 strips. For on-site diagnosis, the two strips reacted positively only to the extractions of the fire-blighted samples on all fire blight occurred orchards except one orchard at which on-site diagnosis was carried out at winter time. In addition, they reacted positively to the black-shoot blighted extractions from the black shoot blight occurred apple orchard. These results suggest that both EB strip and EaAgriStrip would be useful for on-site diagnosis of fire blight in Korea. |
| first_indexed | 2024-12-23T23:18:21Z |
| format | Article |
| id | doaj.art-58aeaeee9585481bb562005022c84ecc |
| institution | Directory Open Access Journal |
| issn | 1598-2262 |
| language | English |
| last_indexed | 2024-12-23T23:18:21Z |
| publishDate | 2017-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | Research in Plant Disease |
| spelling | doaj.art-58aeaeee9585481bb562005022c84ecc2022-12-21T17:26:25ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622017-12-0123430631310.5423/RPD.2017.23.4.306RPD.2017.23.4.306On-Site Diagnosis of Fire Blight with Antibody-Based Diagnostic StripsGwang-Il Heo0Doo-San Shin1Soo-Hyeong Son2Chang-Sik Oh3Duck Hwan Park4Young-Kee Lee5Jae-Soon Cha6Major in Plant Medicine, School of Applied Plant Science & Biotechnology, Chungbuk National University, Cheongju 28644, KoreaMajor in Plant Medicine, School of Applied Plant Science & Biotechnology, Chungbuk National University, Cheongju 28644, KoreaMajor in Plant Medicine, School of Applied Plant Science & Biotechnology, Chungbuk National University, Cheongju 28644, KoreaDepartment of Horticultural Biotechnology, Kyung Hee University, Yongin 17104, KoreaApplied Biology Program, Division of Bioresource Sciences, Kangwon National University, Chuncheon 24341, KoreaDepartment of Agro-food Safety and Crop Protection, National Institute of Agriculture Sciences, Rural Development Administration, Wanju 55365, KoreaMajor in Plant Medicine, School of Applied Plant Science & Biotechnology, Chungbuk National University, Cheongju 28644, KoreaRecently fire blight occurred in the Republic of Korea and eradication program for the disease has been executed since then. Specificity and detection sensitivity of the 2 antibody-based diagnostic strips to Korean isolates of Erwiniaamylovora (Ea) and their application for on-site diagnosis were evaluated in this study. EaAgriStrip, a commercial diagnostic kit, and EB strip, developed in this study, reacted positively to the all tested Korean Ea strains and also to most of Erwiniapyrifoliae (Ep) strains causing black shoot blight. They reacted negatively to all Pusedomonassyringae pv. syringae (Pss) strains that cause shoot blight on apple. Detection sensitivity was similar between the 2 strips. For on-site diagnosis, the two strips reacted positively only to the extractions of the fire-blighted samples on all fire blight occurred orchards except one orchard at which on-site diagnosis was carried out at winter time. In addition, they reacted positively to the black-shoot blighted extractions from the black shoot blight occurred apple orchard. These results suggest that both EB strip and EaAgriStrip would be useful for on-site diagnosis of fire blight in Korea.http://www.online-rpd.org/journal/view.html?doi=10.5423/RPD.2017.23.4.306EaAgriStripEB stripFire blightOn-site diagnosisPlant quarantine pathogen |
| spellingShingle | Gwang-Il Heo Doo-San Shin Soo-Hyeong Son Chang-Sik Oh Duck Hwan Park Young-Kee Lee Jae-Soon Cha On-Site Diagnosis of Fire Blight with Antibody-Based Diagnostic Strips Research in Plant Disease EaAgriStrip EB strip Fire blight On-site diagnosis Plant quarantine pathogen |
| title | On-Site Diagnosis of Fire Blight with Antibody-Based Diagnostic Strips |
| title_full | On-Site Diagnosis of Fire Blight with Antibody-Based Diagnostic Strips |
| title_fullStr | On-Site Diagnosis of Fire Blight with Antibody-Based Diagnostic Strips |
| title_full_unstemmed | On-Site Diagnosis of Fire Blight with Antibody-Based Diagnostic Strips |
| title_short | On-Site Diagnosis of Fire Blight with Antibody-Based Diagnostic Strips |
| title_sort | on site diagnosis of fire blight with antibody based diagnostic strips |
| topic | EaAgriStrip EB strip Fire blight On-site diagnosis Plant quarantine pathogen |
| url | http://www.online-rpd.org/journal/view.html?doi=10.5423/RPD.2017.23.4.306 |
| work_keys_str_mv | AT gwangilheo onsitediagnosisoffireblightwithantibodybaseddiagnosticstrips AT doosanshin onsitediagnosisoffireblightwithantibodybaseddiagnosticstrips AT soohyeongson onsitediagnosisoffireblightwithantibodybaseddiagnosticstrips AT changsikoh onsitediagnosisoffireblightwithantibodybaseddiagnosticstrips AT duckhwanpark onsitediagnosisoffireblightwithantibodybaseddiagnosticstrips AT youngkeelee onsitediagnosisoffireblightwithantibodybaseddiagnosticstrips AT jaesooncha onsitediagnosisoffireblightwithantibodybaseddiagnosticstrips |