Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria
Abstract Background Plasmodium falciparum malaria remains one of the world’s major infectious diseases that cause most morbidity and mortality, particularly in children. In Ghana, most children below the ages of 5 years depending on the severity of the infection often lose their lives. However, it i...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | Malaria Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12936-020-03218-4 |
| _version_ | 1818026193918623744 |
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| author | Renate Asare Clement Opoku-Okrah Kwabena Owusu Danquah Ohene Opare-Sem Otchere Addai-Mensah Daniel Gyamfi Francis Agyei Amponsah Edward Y. Afriyie Richard Vikpebah Duneeh David Ntiamoah Ofosu Michael Frimpong |
| author_facet | Renate Asare Clement Opoku-Okrah Kwabena Owusu Danquah Ohene Opare-Sem Otchere Addai-Mensah Daniel Gyamfi Francis Agyei Amponsah Edward Y. Afriyie Richard Vikpebah Duneeh David Ntiamoah Ofosu Michael Frimpong |
| author_sort | Renate Asare |
| collection | DOAJ |
| description | Abstract Background Plasmodium falciparum malaria remains one of the world’s major infectious diseases that cause most morbidity and mortality, particularly in children. In Ghana, most children below the ages of 5 years depending on the severity of the infection often lose their lives. However, it is still debatable why infection with falciparum malaria contributes to thrombocytopenia. Methods This study sought to investigate the expression of the various platelet indices and activation markers in children with falciparum malaria. Platelet indices (Platelet count [PLT], Plateletcrite [PCT], Mean Platelet Volume [MPV], Platelet Distribution Width [PDW] and Platelet-Large Cell Ratio [P-LCR]) and platelet surface membrane glycoproteins (GPIIb/IIIa [PAC-1], P-selectin [CD62p] and GPIV [CD36]) expressions were determined in children with falciparum malaria (cases) and healthy children (controls) using automated blood cell analysis and flow cytometry techniques, respectively. Results Except for P-LCR, all the other platelet indices (PLT, MPV, PDW, and PCT) were significantly lower in the cases than the controls (P < 0.05). Also, it was observed that the level of expression of the activation markers; PAC 1 and CD62p showed a significant (P < 0.05) decreased before and after activation in falciparum malaria cases than in the controls. On the contrary, CD36 expression in the controls did not differ significantly (p > 0.05) from the malaria cases. Platelet activation markers were known to be associated with increased risk of falciparum malaria with the mean fluorescence intensity of PAC1 (Odds Ratio [OR] 34.0, Relative Risk [RR] 4.47, 95% Confidence Interval [CI] 4.904–235.7; p < 0.0001) and CD36 (OR 4.2, RR 1.82, 95% CI 0.9824–17.96; p = 0.04). Moreover, the percentage expression of CD62p (OR 4.0, RR 1.80, 95% CI 0.59–27.24; p = 0.19) was also observed to be probably associated with increased risk of falciparum malaria although not statistically significant (p > 0.05). Conclusion Plasmodium falciparum malaria has been known to be associated with platelet activation markers, which probably contributes to thrombocytopenia. |
| first_indexed | 2024-12-10T04:28:07Z |
| format | Article |
| id | doaj.art-58b2183373ab4143ad4a49180a5ea5a8 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-10T04:28:07Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-58b2183373ab4143ad4a49180a5ea5a82022-12-22T02:02:13ZengBMCMalaria Journal1475-28752020-04-011911710.1186/s12936-020-03218-4Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malariaRenate Asare0Clement Opoku-Okrah1Kwabena Owusu Danquah2Ohene Opare-Sem3Otchere Addai-Mensah4Daniel Gyamfi5Francis Agyei Amponsah6Edward Y. Afriyie7Richard Vikpebah Duneeh8David Ntiamoah Ofosu9Michael Frimpong10Heamatology Unit, Komfo Anokye Teaching Hospital (KATH)Department of Medical Laboratory Technology, Kwame Nkrumah University of Science and Technology (KNUST)Department of Medical Laboratory Technology, Kwame Nkrumah University of Science and Technology (KNUST)Heamatology Unit, Komfo Anokye Teaching Hospital (KATH)Department of Medical Laboratory Technology, Kwame Nkrumah University of Science and Technology (KNUST)Department of Medical Laboratory Technology, Kwame Nkrumah University of Science and Technology (KNUST)St. John of God HospitalHeamatology Unit, Komfo Anokye Teaching Hospital (KATH)Heamatology Unit, Komfo Anokye Teaching Hospital (KATH)Department of Medical Laboratory Technology, Kwame Nkrumah University of Science and Technology (KNUST)Kumasi Center for Collaborative Research in Tropical Medicine (KCCR)Abstract Background Plasmodium falciparum malaria remains one of the world’s major infectious diseases that cause most morbidity and mortality, particularly in children. In Ghana, most children below the ages of 5 years depending on the severity of the infection often lose their lives. However, it is still debatable why infection with falciparum malaria contributes to thrombocytopenia. Methods This study sought to investigate the expression of the various platelet indices and activation markers in children with falciparum malaria. Platelet indices (Platelet count [PLT], Plateletcrite [PCT], Mean Platelet Volume [MPV], Platelet Distribution Width [PDW] and Platelet-Large Cell Ratio [P-LCR]) and platelet surface membrane glycoproteins (GPIIb/IIIa [PAC-1], P-selectin [CD62p] and GPIV [CD36]) expressions were determined in children with falciparum malaria (cases) and healthy children (controls) using automated blood cell analysis and flow cytometry techniques, respectively. Results Except for P-LCR, all the other platelet indices (PLT, MPV, PDW, and PCT) were significantly lower in the cases than the controls (P < 0.05). Also, it was observed that the level of expression of the activation markers; PAC 1 and CD62p showed a significant (P < 0.05) decreased before and after activation in falciparum malaria cases than in the controls. On the contrary, CD36 expression in the controls did not differ significantly (p > 0.05) from the malaria cases. Platelet activation markers were known to be associated with increased risk of falciparum malaria with the mean fluorescence intensity of PAC1 (Odds Ratio [OR] 34.0, Relative Risk [RR] 4.47, 95% Confidence Interval [CI] 4.904–235.7; p < 0.0001) and CD36 (OR 4.2, RR 1.82, 95% CI 0.9824–17.96; p = 0.04). Moreover, the percentage expression of CD62p (OR 4.0, RR 1.80, 95% CI 0.59–27.24; p = 0.19) was also observed to be probably associated with increased risk of falciparum malaria although not statistically significant (p > 0.05). Conclusion Plasmodium falciparum malaria has been known to be associated with platelet activation markers, which probably contributes to thrombocytopenia.http://link.springer.com/article/10.1186/s12936-020-03218-4Platelet membrane glycoproteinsPlatelet indicesPlasmodium falciparumFlow cytometry |
| spellingShingle | Renate Asare Clement Opoku-Okrah Kwabena Owusu Danquah Ohene Opare-Sem Otchere Addai-Mensah Daniel Gyamfi Francis Agyei Amponsah Edward Y. Afriyie Richard Vikpebah Duneeh David Ntiamoah Ofosu Michael Frimpong Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria Malaria Journal Platelet membrane glycoproteins Platelet indices Plasmodium falciparum Flow cytometry |
| title | Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria |
| title_full | Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria |
| title_fullStr | Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria |
| title_full_unstemmed | Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria |
| title_short | Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria |
| title_sort | assessment of platelet indices and platelet activation markers in children with plasmodium falciparum malaria |
| topic | Platelet membrane glycoproteins Platelet indices Plasmodium falciparum Flow cytometry |
| url | http://link.springer.com/article/10.1186/s12936-020-03218-4 |
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