Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing
ABSTRACT Single-cell microfluidics is a powerful method to study bacteria and determine their susceptibility to antibiotic treatment. Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2019-08-01
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| Series: | mBio |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mBio.01375-19 |
| _version_ | 1818856396994641920 |
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| author | Julie Tréguier Loic Bugnicourt Guillaume Gay Mamoudou Diallo Salim Timo Islam Alexandre Toro Laurent David Olivier Théodoly Guillaume Sudre Tâm Mignot |
| author_facet | Julie Tréguier Loic Bugnicourt Guillaume Gay Mamoudou Diallo Salim Timo Islam Alexandre Toro Laurent David Olivier Théodoly Guillaume Sudre Tâm Mignot |
| author_sort | Julie Tréguier |
| collection | DOAJ |
| description | ABSTRACT Single-cell microfluidics is a powerful method to study bacteria and determine their susceptibility to antibiotic treatment. Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology. In this work, we chemically characterized a class of chitosan polymers for their biocompatibility when adsorbed to glass. Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology. Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods. Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance. IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation. Chitosan films are fully biocompatible and could thus be a viable replacement for existing commercial devices that currently use polylysine. Thus, the low cost of chitosan slides and their simple implementation make them highly versatile for research as well as clinical use. |
| first_indexed | 2024-12-19T08:23:51Z |
| format | Article |
| id | doaj.art-58b6361f9ac54f8a8d385d0b1cce2513 |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-12-19T08:23:51Z |
| publishDate | 2019-08-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-58b6361f9ac54f8a8d385d0b1cce25132022-12-21T20:29:20ZengAmerican Society for MicrobiologymBio2150-75112019-08-0110410.1128/mBio.01375-19Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility TestingJulie Tréguier0Loic Bugnicourt1Guillaume Gay2Mamoudou Diallo3Salim Timo Islam4Alexandre Toro5Laurent David6Olivier Théodoly7Guillaume Sudre8Tâm Mignot9Laboratoire de Chimie Bactérienne, Institut de Microbiologie de la Méditerranée, CNRS-Aix Marseille University (UMR7283), Marseille, FranceIngénierie des Matériaux Polymères, Université Claude Bernard Lyon 1, Université de Lyon, CNRS UMR 5223, Villeurbanne, FranceMorphogénie Logiciels SAS, Marseille, FranceIngénierie des Matériaux Polymères, Université Claude Bernard Lyon 1, Université de Lyon, CNRS UMR 5223, Villeurbanne, FranceLaboratoire de Chimie Bactérienne, Institut de Microbiologie de la Méditerranée, CNRS-Aix Marseille University (UMR7283), Marseille, FranceLaboratoire de Biologie, Centre Hospitalier de Martigues, Martigues, FranceIngénierie des Matériaux Polymères, Université Claude Bernard Lyon 1, Université de Lyon, CNRS UMR 5223, Villeurbanne, FranceLaboratoire Adhésion et Inflammation, INSERM U1067, CNRS UMR 7333, Marseille, FranceIngénierie des Matériaux Polymères, Université Claude Bernard Lyon 1, Université de Lyon, CNRS UMR 5223, Villeurbanne, FranceLaboratoire de Chimie Bactérienne, Institut de Microbiologie de la Méditerranée, CNRS-Aix Marseille University (UMR7283), Marseille, FranceABSTRACT Single-cell microfluidics is a powerful method to study bacteria and determine their susceptibility to antibiotic treatment. Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology. In this work, we chemically characterized a class of chitosan polymers for their biocompatibility when adsorbed to glass. Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology. Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods. Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance. IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation. Chitosan films are fully biocompatible and could thus be a viable replacement for existing commercial devices that currently use polylysine. Thus, the low cost of chitosan slides and their simple implementation make them highly versatile for research as well as clinical use.https://journals.asm.org/doi/10.1128/mBio.01375-19antibiotic susceptibility testingchitosanEscherichia coliKlebsiella pneumoniasingle cellsmicrofluidics |
| spellingShingle | Julie Tréguier Loic Bugnicourt Guillaume Gay Mamoudou Diallo Salim Timo Islam Alexandre Toro Laurent David Olivier Théodoly Guillaume Sudre Tâm Mignot Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing mBio antibiotic susceptibility testing chitosan Escherichia coli Klebsiella pneumonia single cells microfluidics |
| title | Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing |
| title_full | Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing |
| title_fullStr | Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing |
| title_full_unstemmed | Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing |
| title_short | Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing |
| title_sort | chitosan films for microfluidic studies of single bacteria and perspectives for antibiotic susceptibility testing |
| topic | antibiotic susceptibility testing chitosan Escherichia coli Klebsiella pneumonia single cells microfluidics |
| url | https://journals.asm.org/doi/10.1128/mBio.01375-19 |
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