Reducing blood sample hemolysis in the emergency department using S-Monovette® in aspiration mode
Background: Blood sample hemolysis continues to be a significant problem in clinical practice. In vitro hemolysis rates up to 77% have been reported in literature. The use of manual aspiration techniques for blood sampling has previously been shown to reduce the burden of erythrocyte injury in the p...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Practical Laboratory Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551723000094 |
| _version_ | 1797797193558523904 |
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| author | Eunizar Omar John Carson Allen Ahmad Khairil Bin Mohamed Jamil Mohamad Fahamy Koenitz Bin Iskandar Kunzang Norbu Connie Tsang Jocelyn Yin Sameera Ganti Ong Siew Kim Marcus Ong Eng Hock |
| author_facet | Eunizar Omar John Carson Allen Ahmad Khairil Bin Mohamed Jamil Mohamad Fahamy Koenitz Bin Iskandar Kunzang Norbu Connie Tsang Jocelyn Yin Sameera Ganti Ong Siew Kim Marcus Ong Eng Hock |
| author_sort | Eunizar Omar |
| collection | DOAJ |
| description | Background: Blood sample hemolysis continues to be a significant problem in clinical practice. In vitro hemolysis rates up to 77% have been reported in literature. The use of manual aspiration techniques for blood sampling has previously been shown to reduce the burden of erythrocyte injury in the pre-analytical phase compared to the vacuum collection technique. This study compares the hemolysis rates between two blood sampling methods: 5.0 ml BD Vacutainer® SST™ (BDV) and 4.9 ml S-Monovette® serum gel tubes in aspiration mode (SMA). Methods: This was a prospective randomised controlled study conducted in an Emergency department (ED). A convenience sample of 191 adult patients, aged 18–90 years old, presenting at the ED and requiring blood samples for serum electrolyte was included in the study. Paired blood samples were obtained through an intravenous cannula from each patient with randomised order of blood draw using SMA or BDV. Patient data was obtained and hemolysis index (HI), serum lactate dehydrogenase (LDH), and serum potassium (K) levels measured. Results: The adjusted mean HI (35.2 vs 21.5 mg/dL, p < 0.001), serum K (4.38 vs 4.16 mmol/L, p < 0.001) and LDH levels (259.6 vs 228.4 U/L, p < 0.001) were significantly higher in blood samples taken using BDV compared to SMA. The frequency of severely hemolyzed (>150 mg/dL) samples was also higher in blood collected using BDV (16.2%) compared to SMA (0%). Conclusions: The burden of hemolysis in blood samples taken from IV cannulae can be effectively reduced with the use of manual aspiration using the S-Monovette® blood collection system as compared to BD-Vacutainer. |
| first_indexed | 2024-03-13T03:44:31Z |
| format | Article |
| id | doaj.art-58b6445c23c54653b75fd62693ae116a |
| institution | Directory Open Access Journal |
| issn | 2352-5517 |
| language | English |
| last_indexed | 2024-03-13T03:44:31Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj.art-58b6445c23c54653b75fd62693ae116a2023-06-23T04:43:16ZengElsevierPractical Laboratory Medicine2352-55172023-05-0135e00315Reducing blood sample hemolysis in the emergency department using S-Monovette® in aspiration modeEunizar Omar0John Carson Allen1Ahmad Khairil Bin Mohamed Jamil2Mohamad Fahamy Koenitz Bin Iskandar3Kunzang Norbu4Connie Tsang5Jocelyn Yin6Sameera Ganti7Ong Siew Kim8Marcus Ong Eng Hock9Department of Emergency Medicine, Sengkang General Hospital, Singapore; Corresponding author.Centre for Quantitative Medicine, Singapore General Hospital, SingaporeDepartment of Emergency Medicine, Sengkang General Hospital, SingaporeDepartment of Emergency Medicine, Sengkang General Hospital, SingaporeDepartment of Emergency Medicine, Sengkang General Hospital, SingaporeDepartment of Emergency Medicine, Sengkang General Hospital, SingaporeDepartment of Emergency Medicine, Sengkang General Hospital, SingaporeDepartment of Emergency Medicine, Sengkang General Hospital, SingaporeDepartment of Pathology, Sengkang General Hospital, SingaporeDuke-NUS Medical School, Singapore; Department of Emergency Medicine, Singapore General Hospital, SingaporeBackground: Blood sample hemolysis continues to be a significant problem in clinical practice. In vitro hemolysis rates up to 77% have been reported in literature. The use of manual aspiration techniques for blood sampling has previously been shown to reduce the burden of erythrocyte injury in the pre-analytical phase compared to the vacuum collection technique. This study compares the hemolysis rates between two blood sampling methods: 5.0 ml BD Vacutainer® SST™ (BDV) and 4.9 ml S-Monovette® serum gel tubes in aspiration mode (SMA). Methods: This was a prospective randomised controlled study conducted in an Emergency department (ED). A convenience sample of 191 adult patients, aged 18–90 years old, presenting at the ED and requiring blood samples for serum electrolyte was included in the study. Paired blood samples were obtained through an intravenous cannula from each patient with randomised order of blood draw using SMA or BDV. Patient data was obtained and hemolysis index (HI), serum lactate dehydrogenase (LDH), and serum potassium (K) levels measured. Results: The adjusted mean HI (35.2 vs 21.5 mg/dL, p < 0.001), serum K (4.38 vs 4.16 mmol/L, p < 0.001) and LDH levels (259.6 vs 228.4 U/L, p < 0.001) were significantly higher in blood samples taken using BDV compared to SMA. The frequency of severely hemolyzed (>150 mg/dL) samples was also higher in blood collected using BDV (16.2%) compared to SMA (0%). Conclusions: The burden of hemolysis in blood samples taken from IV cannulae can be effectively reduced with the use of manual aspiration using the S-Monovette® blood collection system as compared to BD-Vacutainer.http://www.sciencedirect.com/science/article/pii/S2352551723000094Vacuum collection systemHemolysisBlood samplingIntravenous cannula |
| spellingShingle | Eunizar Omar John Carson Allen Ahmad Khairil Bin Mohamed Jamil Mohamad Fahamy Koenitz Bin Iskandar Kunzang Norbu Connie Tsang Jocelyn Yin Sameera Ganti Ong Siew Kim Marcus Ong Eng Hock Reducing blood sample hemolysis in the emergency department using S-Monovette® in aspiration mode Practical Laboratory Medicine Vacuum collection system Hemolysis Blood sampling Intravenous cannula |
| title | Reducing blood sample hemolysis in the emergency department using S-Monovette® in aspiration mode |
| title_full | Reducing blood sample hemolysis in the emergency department using S-Monovette® in aspiration mode |
| title_fullStr | Reducing blood sample hemolysis in the emergency department using S-Monovette® in aspiration mode |
| title_full_unstemmed | Reducing blood sample hemolysis in the emergency department using S-Monovette® in aspiration mode |
| title_short | Reducing blood sample hemolysis in the emergency department using S-Monovette® in aspiration mode |
| title_sort | reducing blood sample hemolysis in the emergency department using s monovette r in aspiration mode |
| topic | Vacuum collection system Hemolysis Blood sampling Intravenous cannula |
| url | http://www.sciencedirect.com/science/article/pii/S2352551723000094 |
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