Inclusion of <i>Citrullus colocynthis</i> Seed Extract into Diets Induced a Hypolipidemic Effect and Improved Layer Performance
<i>Citrullus colocynthis</i> (CC) has been known as a natural medicinal plant with wide biological activities, including antioxidant, anti-inflammatory, and antilipidemic effects. The aim of this study was to investigate the effect of inclusion of the ethanolic extract of CC seeds (ECCs)...
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MDPI AG
2021-08-01
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| author | Mohamed I. Alzarah Abdulaziz A. Alaqil Ahmed O. Abbas Farid S. Nassar Gamal M. K. Mehaisen Gouda F. Gouda Hanaa K. Abd El-Atty Eman S. Moustafa |
| author_facet | Mohamed I. Alzarah Abdulaziz A. Alaqil Ahmed O. Abbas Farid S. Nassar Gamal M. K. Mehaisen Gouda F. Gouda Hanaa K. Abd El-Atty Eman S. Moustafa |
| author_sort | Mohamed I. Alzarah |
| collection | DOAJ |
| description | <i>Citrullus colocynthis</i> (CC) has been known as a natural medicinal plant with wide biological activities, including antioxidant, anti-inflammatory, and antilipidemic effects. The aim of this study was to investigate the effect of inclusion of the ethanolic extract of CC seeds (ECCs) into layer diets on the lipid profile, stress indicators, and physiological and productive performance of laying hens. A total of 216 forty-week-old commercial Hy-Line brown laying hens were randomly assigned into four equal groups (3 birds × 18 replicates per group) that received a basal diet supplemented with 0, 0.5, 1.0, and 2.0 g/kg of ECCs for 12 consecutive weeks. The first group served as a control. The results showed that ECCs at 1.0 and 2.0 g/kg significantly (<i>p</i> < 0.05) improved the productive and physiological performance compared to the other groups. In addition, stress indicators examined in the laying hens, including lipid peroxidation (malondialdehyde (MDA)), corticosterone hormone (CORT), tumor necrosis factor alpha (TNFα), and heat shock protein 70 (HSP70), were significantly alleviated after inclusion of ECCs into layer diets at the three levels compared to the control group. Furthermore, all ECC levels induced a significant reduction in plasma triglyceride (TG) and cholesterol (CH) levels in the plasma, liver, and egg yolk, whereas the highest levels were obtained with 2.0 g/kg of ECCs. Particularly important, a high linear correlation (R<sup>2</sup> = 0.60–0.79) was observed between increasing doses of ECCs and MDA, liver CH, and egg yolk CH concentrations and egg weight, feed intake, and feed conversion ratio; moreover, the correlation was extremely high (R<sup>2</sup> = 0.80–0.100) with the level of TG, CH, low-density lipoprotein CH, high-density lipoprotein CH, and CORT. These results indicated that dietary supplementation with 2.0 g/kg of ECCs could be considered a successful nutritional approach to producing healthier, lower-cholesterol eggs for consumers, in addition to enhancing the physiological and productive performance of laying hens by alleviating the stress of intensive commercial production. |
| first_indexed | 2024-03-10T07:59:00Z |
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| last_indexed | 2024-03-10T07:59:00Z |
| publishDate | 2021-08-01 |
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| spelling | doaj.art-58b8ab48ef7541a0ad0f981783f1e6002023-11-22T11:34:53ZengMDPI AGAgriculture2077-04722021-08-0111980810.3390/agriculture11090808Inclusion of <i>Citrullus colocynthis</i> Seed Extract into Diets Induced a Hypolipidemic Effect and Improved Layer PerformanceMohamed I. Alzarah0Abdulaziz A. Alaqil1Ahmed O. Abbas2Farid S. Nassar3Gamal M. K. Mehaisen4Gouda F. Gouda5Hanaa K. Abd El-Atty6Eman S. Moustafa7Department of Environmental and Natural Resources, College of Agricultural and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi ArabiaDepartment of Animal and Fish Production, College of Agricultural and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi ArabiaDepartment of Animal and Fish Production, College of Agricultural and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi ArabiaDepartment of Animal Production, Faculty of Agriculture, Cairo University, Gamma Street, Giza 12613, EgyptDepartment of Animal Production, Faculty of Agriculture, Cairo University, Gamma Street, Giza 12613, EgyptAnimal Breeding Section, Department of Animal Production, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Haddaik Shoubra 11241, Cairo, EgyptDepartment of Poultry Breeding, Animal Production Research Institute, Agricultural Research Center, Dokki, Giza 12611, EgyptDepartment of Animal Production, Faculty of Agriculture, Cairo University, Gamma Street, Giza 12613, Egypt<i>Citrullus colocynthis</i> (CC) has been known as a natural medicinal plant with wide biological activities, including antioxidant, anti-inflammatory, and antilipidemic effects. The aim of this study was to investigate the effect of inclusion of the ethanolic extract of CC seeds (ECCs) into layer diets on the lipid profile, stress indicators, and physiological and productive performance of laying hens. A total of 216 forty-week-old commercial Hy-Line brown laying hens were randomly assigned into four equal groups (3 birds × 18 replicates per group) that received a basal diet supplemented with 0, 0.5, 1.0, and 2.0 g/kg of ECCs for 12 consecutive weeks. The first group served as a control. The results showed that ECCs at 1.0 and 2.0 g/kg significantly (<i>p</i> < 0.05) improved the productive and physiological performance compared to the other groups. In addition, stress indicators examined in the laying hens, including lipid peroxidation (malondialdehyde (MDA)), corticosterone hormone (CORT), tumor necrosis factor alpha (TNFα), and heat shock protein 70 (HSP70), were significantly alleviated after inclusion of ECCs into layer diets at the three levels compared to the control group. Furthermore, all ECC levels induced a significant reduction in plasma triglyceride (TG) and cholesterol (CH) levels in the plasma, liver, and egg yolk, whereas the highest levels were obtained with 2.0 g/kg of ECCs. Particularly important, a high linear correlation (R<sup>2</sup> = 0.60–0.79) was observed between increasing doses of ECCs and MDA, liver CH, and egg yolk CH concentrations and egg weight, feed intake, and feed conversion ratio; moreover, the correlation was extremely high (R<sup>2</sup> = 0.80–0.100) with the level of TG, CH, low-density lipoprotein CH, high-density lipoprotein CH, and CORT. These results indicated that dietary supplementation with 2.0 g/kg of ECCs could be considered a successful nutritional approach to producing healthier, lower-cholesterol eggs for consumers, in addition to enhancing the physiological and productive performance of laying hens by alleviating the stress of intensive commercial production.https://www.mdpi.com/2077-0472/11/9/808<i>Citrullus colocynthis</i>lipid profilestress indicatorsphysiological statusproductive performancelaying hens |
| spellingShingle | Mohamed I. Alzarah Abdulaziz A. Alaqil Ahmed O. Abbas Farid S. Nassar Gamal M. K. Mehaisen Gouda F. Gouda Hanaa K. Abd El-Atty Eman S. Moustafa Inclusion of <i>Citrullus colocynthis</i> Seed Extract into Diets Induced a Hypolipidemic Effect and Improved Layer Performance Agriculture <i>Citrullus colocynthis</i> lipid profile stress indicators physiological status productive performance laying hens |
| title | Inclusion of <i>Citrullus colocynthis</i> Seed Extract into Diets Induced a Hypolipidemic Effect and Improved Layer Performance |
| title_full | Inclusion of <i>Citrullus colocynthis</i> Seed Extract into Diets Induced a Hypolipidemic Effect and Improved Layer Performance |
| title_fullStr | Inclusion of <i>Citrullus colocynthis</i> Seed Extract into Diets Induced a Hypolipidemic Effect and Improved Layer Performance |
| title_full_unstemmed | Inclusion of <i>Citrullus colocynthis</i> Seed Extract into Diets Induced a Hypolipidemic Effect and Improved Layer Performance |
| title_short | Inclusion of <i>Citrullus colocynthis</i> Seed Extract into Diets Induced a Hypolipidemic Effect and Improved Layer Performance |
| title_sort | inclusion of i citrullus colocynthis i seed extract into diets induced a hypolipidemic effect and improved layer performance |
| topic | <i>Citrullus colocynthis</i> lipid profile stress indicators physiological status productive performance laying hens |
| url | https://www.mdpi.com/2077-0472/11/9/808 |
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