Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism
Background: Globally, obesity is a significant public problem, especially when aging. Sesamol, a phenolic lignan present in sesame seeds, might have a positive effect on high-fat diet (HFD)-induced obesity associated with aging. Objective: The purpose of current research study was to explore salutar...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Swedish Nutrition Foundation
2022-03-01
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| Series: | Food & Nutrition Research |
| Subjects: | |
| Online Access: | https://foodandnutritionresearch.net/index.php/fnr/article/view/8231/14450 |
| _version_ | 1819061983800983552 |
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| author | Min-Min Hu Ji-Hua Chen Quan-Quan Zhang Zi-Yu Song Horia Shaukat Hong Qin |
| author_facet | Min-Min Hu Ji-Hua Chen Quan-Quan Zhang Zi-Yu Song Horia Shaukat Hong Qin |
| author_sort | Min-Min Hu |
| collection | DOAJ |
| description | Background: Globally, obesity is a significant public problem, especially when aging. Sesamol, a phenolic lignan present in sesame seeds, might have a positive effect on high-fat diet (HFD)-induced obesity associated with aging. Objective: The purpose of current research study was to explore salutary effects and mechanisms of sesamol in treating alimentary obesity and associated metabolic syndrome in middle-aged mice. Methods: C57BL/6J mice aged 4–6 weeks and 6–8 months were assigned to the young normal diet group, middle-aged normal diet group, middle-aged HFD group, and middle-aged HFD + sesamol group. At the end of experiment, glucose tolerance test and insulin tolerance test were performed; the levels of lipids and oxidative stress-related factors in the serum and skeletal muscle were detected using chemistry reagent kits; lipid accumulation in skeletal muscle was observed by oil red O staining; the expressions of muscular glucose and lipid metabolism associated proteins were measured by Western blotting. Results: Sesamol decreased the body weight and alleviated obesity-associated metabolism syndrome in middle-aged mice, such as glucose intolerance, insulin resistance, dyslipidemia, and oxidative stress. Moreover, muscular metabolic disorders were attenuated after treatment with sesamol. It increased the expression of glucose transporter type-4 and down-regulated the protein levels of pyruvate dehydrogenase kinase isozyme 4, implying the increase of glucose uptake and oxidation. Meanwhile, sesamol decreased the expression of sterol regulatory element binding protein 1c and up-regulated the phosphorylation of hormone-sensitive lipase and the level of carnitine palmityl transferase 1α, which led to the declined lipogenesis and the increased lipolysis and lipid oxidation. In addition, the SIRT1/AMPK signaling pathway was triggered by sesamol, from which it is understood how sesamol enhances glucose and lipid metabolism. Conclusions: Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism, which might be associated with the stimulation of the SIRT1/AMPK pathway. |
| first_indexed | 2024-12-21T14:51:33Z |
| format | Article |
| id | doaj.art-58bb3a2cf81746c4a0e3c6bcda262492 |
| institution | Directory Open Access Journal |
| issn | 1654-661X |
| language | English |
| last_indexed | 2024-12-21T14:51:33Z |
| publishDate | 2022-03-01 |
| publisher | Swedish Nutrition Foundation |
| record_format | Article |
| series | Food & Nutrition Research |
| spelling | doaj.art-58bb3a2cf81746c4a0e3c6bcda2624922022-12-21T18:59:51ZengSwedish Nutrition FoundationFood & Nutrition Research1654-661X2022-03-016611310.29219/fnr.v66.82318231Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolismMin-Min Hu0Ji-Hua Chen1Quan-Quan Zhang2Zi-Yu Song3Horia Shaukat4Hong Qin5Department of Nutrition Science and Food Hygiene, Xiangya School of Public Health, Central South University, Changsha, ChinaDepartment of Nutrition Science and Food Hygiene, Xiangya School of Public Health, Central South University, Changsha, ChinaDepartment of Nutrition Science and Food Hygiene, Xiangya School of Public Health, Central South University, Changsha, ChinaDepartment of Nutrition Science and Food Hygiene, Xiangya School of Public Health, Central South University, Changsha, ChinaDepartment of Nutrition Science and Food Hygiene, Xiangya School of Public Health, Central South University, Changsha, ChinaDepartment of Nutrition Science and Food Hygiene, Xiangya School of Public Health, Central South University, Changsha, ChinaBackground: Globally, obesity is a significant public problem, especially when aging. Sesamol, a phenolic lignan present in sesame seeds, might have a positive effect on high-fat diet (HFD)-induced obesity associated with aging. Objective: The purpose of current research study was to explore salutary effects and mechanisms of sesamol in treating alimentary obesity and associated metabolic syndrome in middle-aged mice. Methods: C57BL/6J mice aged 4–6 weeks and 6–8 months were assigned to the young normal diet group, middle-aged normal diet group, middle-aged HFD group, and middle-aged HFD + sesamol group. At the end of experiment, glucose tolerance test and insulin tolerance test were performed; the levels of lipids and oxidative stress-related factors in the serum and skeletal muscle were detected using chemistry reagent kits; lipid accumulation in skeletal muscle was observed by oil red O staining; the expressions of muscular glucose and lipid metabolism associated proteins were measured by Western blotting. Results: Sesamol decreased the body weight and alleviated obesity-associated metabolism syndrome in middle-aged mice, such as glucose intolerance, insulin resistance, dyslipidemia, and oxidative stress. Moreover, muscular metabolic disorders were attenuated after treatment with sesamol. It increased the expression of glucose transporter type-4 and down-regulated the protein levels of pyruvate dehydrogenase kinase isozyme 4, implying the increase of glucose uptake and oxidation. Meanwhile, sesamol decreased the expression of sterol regulatory element binding protein 1c and up-regulated the phosphorylation of hormone-sensitive lipase and the level of carnitine palmityl transferase 1α, which led to the declined lipogenesis and the increased lipolysis and lipid oxidation. In addition, the SIRT1/AMPK signaling pathway was triggered by sesamol, from which it is understood how sesamol enhances glucose and lipid metabolism. Conclusions: Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism, which might be associated with the stimulation of the SIRT1/AMPK pathway.https://foodandnutritionresearch.net/index.php/fnr/article/view/8231/14450sesamolobesityagingskeletal muscleglucose metabolismlipid metabolism |
| spellingShingle | Min-Min Hu Ji-Hua Chen Quan-Quan Zhang Zi-Yu Song Horia Shaukat Hong Qin Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism Food & Nutrition Research sesamol obesity aging skeletal muscle glucose metabolism lipid metabolism |
| title | Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism |
| title_full | Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism |
| title_fullStr | Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism |
| title_full_unstemmed | Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism |
| title_short | Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism |
| title_sort | sesamol counteracts on metabolic disorders of middle aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism |
| topic | sesamol obesity aging skeletal muscle glucose metabolism lipid metabolism |
| url | https://foodandnutritionresearch.net/index.php/fnr/article/view/8231/14450 |
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