Peculiar Expression of CD3-Epsilon in Kidney of Ginbuna Crucian Carp
TCR/CD3 complex is composed of the disulfide-linked TCR-αβ heterodimer that recognizes the antigen as a peptide presented by the MHC, and non-covalently paired CD3γε- and δε-chains together with disulfide-linked ζ-chain homodimers. The CD3 chains play key roles in T cell development and T cell activ...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-06-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2018.01321/full |
| _version_ | 1818560991955255296 |
|---|---|
| author | Ryuichiro Miyazawa Norifumi Murata Yuta Matsuura Yasuhiro Shibasaki Takeshi Yabu Teruyuki Nakanishi |
| author_facet | Ryuichiro Miyazawa Norifumi Murata Yuta Matsuura Yasuhiro Shibasaki Takeshi Yabu Teruyuki Nakanishi |
| author_sort | Ryuichiro Miyazawa |
| collection | DOAJ |
| description | TCR/CD3 complex is composed of the disulfide-linked TCR-αβ heterodimer that recognizes the antigen as a peptide presented by the MHC, and non-covalently paired CD3γε- and δε-chains together with disulfide-linked ζ-chain homodimers. The CD3 chains play key roles in T cell development and T cell activation. In the present study, we found nor or extremely lower expression of CD3ε in head- and trunk-kidney lymphocytes by flow cytometric analysis, while CD3ε was expressed at the normal level in lymphocytes from thymus, spleen, intestine, gill, and peripheral blood. Furthermore, CD4-1+ and CD8α+ T cells from kidney express Zap-70, but not CD3ε, while the T cells from other tissues express both Zap-70 and CD3ε, although expression of CD3ε was low. Quantitative analysis of mRNA expression revealed that the expression level of T cell-related genes including tcrb, cd3ε, zap-70, and lck in CD4-1+ and CD8α+ T cells was not different between kidney and spleen. Western blot analysis showed that CD3ε band was detected in the cell lysates of spleen but not kidney. To be interested, CD3ε-positive cells greatly increased after 24 h in in vitro culture of kidney leukocytes. Furthermore, expression of CD3ε in both transferred kidney and spleen leukocytes was not detected or very low in kidney, while both leukocytes expressed CD3ε at normal level in spleen when kidney and spleen leukocytes were injected into the isogeneic recipient. Lower expression of CD3ε was also found in kidney T lymphocytes of goldfish and carp. These results indicate that kidney lymphocytes express no or lower level of CD3ε protein in the kidney, although the mRNA of the gene was expressed. Here, we discuss this phenomenon from the point of function of kidney as reservoir for T lymphocytes in teleost, which lacks lymph node and bone marrow. |
| first_indexed | 2024-12-14T00:45:21Z |
| format | Article |
| id | doaj.art-58bca4f9d72e454aa976a7c86922fe5b |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-14T00:45:21Z |
| publishDate | 2018-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-58bca4f9d72e454aa976a7c86922fe5b2022-12-21T23:24:10ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-06-01910.3389/fimmu.2018.01321360101Peculiar Expression of CD3-Epsilon in Kidney of Ginbuna Crucian CarpRyuichiro Miyazawa0Norifumi Murata1Yuta Matsuura2Yasuhiro Shibasaki3Takeshi Yabu4Teruyuki Nakanishi5Department of Veterinary Medicine, Nihon University, Fujisawa, JapanDepartment of Veterinary Medicine, Nihon University, Fujisawa, JapanResearch Center for Fish Diseases, National Research Institute of Aquaculture, Minami-ise, JapanDepartment of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, United StatesDepartment of Applied Biological Science, Nihon University, Fujisawa, JapanDepartment of Veterinary Medicine, Nihon University, Fujisawa, JapanTCR/CD3 complex is composed of the disulfide-linked TCR-αβ heterodimer that recognizes the antigen as a peptide presented by the MHC, and non-covalently paired CD3γε- and δε-chains together with disulfide-linked ζ-chain homodimers. The CD3 chains play key roles in T cell development and T cell activation. In the present study, we found nor or extremely lower expression of CD3ε in head- and trunk-kidney lymphocytes by flow cytometric analysis, while CD3ε was expressed at the normal level in lymphocytes from thymus, spleen, intestine, gill, and peripheral blood. Furthermore, CD4-1+ and CD8α+ T cells from kidney express Zap-70, but not CD3ε, while the T cells from other tissues express both Zap-70 and CD3ε, although expression of CD3ε was low. Quantitative analysis of mRNA expression revealed that the expression level of T cell-related genes including tcrb, cd3ε, zap-70, and lck in CD4-1+ and CD8α+ T cells was not different between kidney and spleen. Western blot analysis showed that CD3ε band was detected in the cell lysates of spleen but not kidney. To be interested, CD3ε-positive cells greatly increased after 24 h in in vitro culture of kidney leukocytes. Furthermore, expression of CD3ε in both transferred kidney and spleen leukocytes was not detected or very low in kidney, while both leukocytes expressed CD3ε at normal level in spleen when kidney and spleen leukocytes were injected into the isogeneic recipient. Lower expression of CD3ε was also found in kidney T lymphocytes of goldfish and carp. These results indicate that kidney lymphocytes express no or lower level of CD3ε protein in the kidney, although the mRNA of the gene was expressed. Here, we discuss this phenomenon from the point of function of kidney as reservoir for T lymphocytes in teleost, which lacks lymph node and bone marrow.https://www.frontiersin.org/article/10.3389/fimmu.2018.01321/fullCD3-epsillonCD4CD8kidneyteleostginbuna crucian carp |
| spellingShingle | Ryuichiro Miyazawa Norifumi Murata Yuta Matsuura Yasuhiro Shibasaki Takeshi Yabu Teruyuki Nakanishi Peculiar Expression of CD3-Epsilon in Kidney of Ginbuna Crucian Carp Frontiers in Immunology CD3-epsillon CD4 CD8 kidney teleost ginbuna crucian carp |
| title | Peculiar Expression of CD3-Epsilon in Kidney of Ginbuna Crucian Carp |
| title_full | Peculiar Expression of CD3-Epsilon in Kidney of Ginbuna Crucian Carp |
| title_fullStr | Peculiar Expression of CD3-Epsilon in Kidney of Ginbuna Crucian Carp |
| title_full_unstemmed | Peculiar Expression of CD3-Epsilon in Kidney of Ginbuna Crucian Carp |
| title_short | Peculiar Expression of CD3-Epsilon in Kidney of Ginbuna Crucian Carp |
| title_sort | peculiar expression of cd3 epsilon in kidney of ginbuna crucian carp |
| topic | CD3-epsillon CD4 CD8 kidney teleost ginbuna crucian carp |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2018.01321/full |
| work_keys_str_mv | AT ryuichiromiyazawa peculiarexpressionofcd3epsiloninkidneyofginbunacruciancarp AT norifumimurata peculiarexpressionofcd3epsiloninkidneyofginbunacruciancarp AT yutamatsuura peculiarexpressionofcd3epsiloninkidneyofginbunacruciancarp AT yasuhiroshibasaki peculiarexpressionofcd3epsiloninkidneyofginbunacruciancarp AT takeshiyabu peculiarexpressionofcd3epsiloninkidneyofginbunacruciancarp AT teruyukinakanishi peculiarexpressionofcd3epsiloninkidneyofginbunacruciancarp |