Regulation of Tumor Necrosis Factor-related Apoptosis-inducing Ligand Expression in Primary Acute Leukemic Cells by Chemotherapeutics
Objective: The expression of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) protein and its regulation by chemotherapeutics were analyzed in primary acute leukemic cells. Materials and Methods: Peripheral blood was collected from 16 patients with acute leukemia on days 0, 1,...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Galenos Publishing House
2013-09-01
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| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-98624 |
| _version_ | 1828018562920349696 |
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| author | Shengmei Chen Yanfang Liu Hui Sun Ling Sun Jie Ma Dingming Wan Zhongxing Jiang Qiutang Zhang Tao Li |
| author_facet | Shengmei Chen Yanfang Liu Hui Sun Ling Sun Jie Ma Dingming Wan Zhongxing Jiang Qiutang Zhang Tao Li |
| author_sort | Shengmei Chen |
| collection | DOAJ |
| description | Objective: The expression of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) protein and its regulation by chemotherapeutics were analyzed in primary acute leukemic cells.
Materials and Methods: Peripheral blood was collected from 16 patients with acute leukemia on days 0, 1, 3, and 5 of chemotherapy. The mononuclear cells were separated from the peripheral blood, and TRAIL expression was assessed by flow cytometry. The bone marrow mononuclear cells of patients with acute leukemia were separated before chemotherapy and cultured in vitro with VP-16 and/or interferon (IFN). The TRAIL expression level was detected after the cell culture.
Results: TRAIL expression in the mononuclear cells of peripheral blood was significantly upregulated on day 1 (p<0.05) and then significantly decreased on day 5 after chemotherapy (p<0.05). Results from the in vitro culture revealed that VP-16 upregulated TRAIL expression in the bone marrow mononuclear cells of patients with acute leukemia, but the binding of VP-16 to IFN did not enhance TRAIL expression as compared with VP-16 alone (p>0.05).
Conclusion: OA single chemotherapy mechanism for leukemia may suffice to induce TRAIL expression and promote the apoptosis of leukemic cells. |
| first_indexed | 2024-04-10T11:06:30Z |
| format | Article |
| id | doaj.art-58c284645a3449aba6c2455c0082dc6e |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T11:06:30Z |
| publishDate | 2013-09-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-58c284645a3449aba6c2455c0082dc6e2023-02-15T16:19:22ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632013-09-0130326326810.4274/Tjh.2013.0027TJH-98624Regulation of Tumor Necrosis Factor-related Apoptosis-inducing Ligand Expression in Primary Acute Leukemic Cells by ChemotherapeuticsShengmei Chen0Yanfang Liu1Hui Sun2Ling Sun3Jie Ma4Dingming Wan5Zhongxing Jiang6Qiutang Zhang7Tao Li8Key Observation Laboratory İn Henan Medical University, Department Of Hematology First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, P.r. ChinaKey Observation Laboratory İn Henan Medical University, Department Of Hematology First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, P.r. ChinaKey Observation Laboratory İn Henan Medical University, Department Of Hematology First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, P.r. ChinaKey Observation Laboratory İn Henan Medical University, Department Of Hematology First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, P.r. ChinaKey Observation Laboratory İn Henan Medical University, Department Of Hematology First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, P.r. ChinaKey Observation Laboratory İn Henan Medical University, Department Of Hematology First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, P.r. ChinaKey Observation Laboratory İn Henan Medical University, Department Of Hematology First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, P.r. ChinaKey Observation Laboratory İn Henan Medical University, Department Of Hematology First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, P.r. ChinaKey Observation Laboratory İn Henan Medical University, Department Of Hematology First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, P.r. ChinaObjective: The expression of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) protein and its regulation by chemotherapeutics were analyzed in primary acute leukemic cells. Materials and Methods: Peripheral blood was collected from 16 patients with acute leukemia on days 0, 1, 3, and 5 of chemotherapy. The mononuclear cells were separated from the peripheral blood, and TRAIL expression was assessed by flow cytometry. The bone marrow mononuclear cells of patients with acute leukemia were separated before chemotherapy and cultured in vitro with VP-16 and/or interferon (IFN). The TRAIL expression level was detected after the cell culture. Results: TRAIL expression in the mononuclear cells of peripheral blood was significantly upregulated on day 1 (p<0.05) and then significantly decreased on day 5 after chemotherapy (p<0.05). Results from the in vitro culture revealed that VP-16 upregulated TRAIL expression in the bone marrow mononuclear cells of patients with acute leukemia, but the binding of VP-16 to IFN did not enhance TRAIL expression as compared with VP-16 alone (p>0.05). Conclusion: OA single chemotherapy mechanism for leukemia may suffice to induce TRAIL expression and promote the apoptosis of leukemic cells.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-98624trailacute leukemiachemotherapeutics |
| spellingShingle | Shengmei Chen Yanfang Liu Hui Sun Ling Sun Jie Ma Dingming Wan Zhongxing Jiang Qiutang Zhang Tao Li Regulation of Tumor Necrosis Factor-related Apoptosis-inducing Ligand Expression in Primary Acute Leukemic Cells by Chemotherapeutics Turkish Journal of Hematology trail acute leukemia chemotherapeutics |
| title | Regulation of Tumor Necrosis Factor-related Apoptosis-inducing Ligand Expression in Primary Acute Leukemic Cells by Chemotherapeutics |
| title_full | Regulation of Tumor Necrosis Factor-related Apoptosis-inducing Ligand Expression in Primary Acute Leukemic Cells by Chemotherapeutics |
| title_fullStr | Regulation of Tumor Necrosis Factor-related Apoptosis-inducing Ligand Expression in Primary Acute Leukemic Cells by Chemotherapeutics |
| title_full_unstemmed | Regulation of Tumor Necrosis Factor-related Apoptosis-inducing Ligand Expression in Primary Acute Leukemic Cells by Chemotherapeutics |
| title_short | Regulation of Tumor Necrosis Factor-related Apoptosis-inducing Ligand Expression in Primary Acute Leukemic Cells by Chemotherapeutics |
| title_sort | regulation of tumor necrosis factor related apoptosis inducing ligand expression in primary acute leukemic cells by chemotherapeutics |
| topic | trail acute leukemia chemotherapeutics |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-98624 |
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