Identification of serum microRNAs as diagnostic biomarkers for schizophrenia
Abstract Background At present, the schizophrenia diagnoses are based on the clinical symptoms and behaviors neglecting the laboratory test indicators. Results To better investigate the diagnostic potential of miRNAs for schizophrenia, we selected 14 candidate miRNAs and examined their expressions i...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | Hereditas |
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| Online Access: | http://link.springer.com/article/10.1186/s41065-019-0099-3 |
| _version_ | 1819296203528994816 |
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| author | Kuanjun He Chuang Guo Meng Guo Shuping Tong Qiuli Zhang Hongjun Sun Lin He Yongyong Shi |
| author_facet | Kuanjun He Chuang Guo Meng Guo Shuping Tong Qiuli Zhang Hongjun Sun Lin He Yongyong Shi |
| author_sort | Kuanjun He |
| collection | DOAJ |
| description | Abstract Background At present, the schizophrenia diagnoses are based on the clinical symptoms and behaviors neglecting the laboratory test indicators. Results To better investigate the diagnostic potential of miRNAs for schizophrenia, we selected 14 candidate miRNAs and examined their expressions in the serums of 40 schizophrenia patients and 40 healthy controls by qRT-PCR. Ultimately three abnormally expressed microRNAs were identified, i.e., miR-34a-5p, miR-432-5p and miR-449a. Then, binary regression analysis was employed to combine 3 dysregulated miRNAs. ROC analysis revealed that the AUC of the combination of miR-432-5p + miR-449a in serums was 0.841 (95% CI: 0.791~0.887) with 90% sensitivity and 80% specificity. The AUC of the combination of miR-34a-5p + miR-432-5p + miR-449a in serums was 0.843 (95% CI: 0.791~0.887) with 90% sensitivity and 77.5% specificity. The results indicated that the combined model of miR-432-5p + miR-449a and miR-34a-5p + miR-432-5p + miR-449a have better prediction performances. Conclusions The study concludes that the two miRNAs combinations have the potential to be used as biomarkers for schizophrenia diagnoses. The finding may be conducive to overcoming the dilemmas faced by current schizophrenia diagnosis. |
| first_indexed | 2024-12-24T04:54:23Z |
| format | Article |
| id | doaj.art-58c2ea64ad3f4bacb46b115f162f849d |
| institution | Directory Open Access Journal |
| issn | 1601-5223 |
| language | English |
| last_indexed | 2024-12-24T04:54:23Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Hereditas |
| spelling | doaj.art-58c2ea64ad3f4bacb46b115f162f849d2022-12-21T17:14:26ZengBMCHereditas1601-52232019-06-0115611710.1186/s41065-019-0099-3Identification of serum microRNAs as diagnostic biomarkers for schizophreniaKuanjun He0Chuang Guo1Meng Guo2Shuping Tong3Qiuli Zhang4Hongjun Sun5Lin He6Yongyong Shi7College of Life Science, Inner Mongolia University for NationalitiesCollege of Life Science, Inner Mongolia University for NationalitiesNetwork Center, Inner Mongolia University for NationalitiesAffiliated Hospital of Inner Mongolia University for NationalitiesAffiliated Hospital of Inner Mongolia University for NationalitiesTongliao Institute of Mental HealthBio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong UniversityBio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong UniversityAbstract Background At present, the schizophrenia diagnoses are based on the clinical symptoms and behaviors neglecting the laboratory test indicators. Results To better investigate the diagnostic potential of miRNAs for schizophrenia, we selected 14 candidate miRNAs and examined their expressions in the serums of 40 schizophrenia patients and 40 healthy controls by qRT-PCR. Ultimately three abnormally expressed microRNAs were identified, i.e., miR-34a-5p, miR-432-5p and miR-449a. Then, binary regression analysis was employed to combine 3 dysregulated miRNAs. ROC analysis revealed that the AUC of the combination of miR-432-5p + miR-449a in serums was 0.841 (95% CI: 0.791~0.887) with 90% sensitivity and 80% specificity. The AUC of the combination of miR-34a-5p + miR-432-5p + miR-449a in serums was 0.843 (95% CI: 0.791~0.887) with 90% sensitivity and 77.5% specificity. The results indicated that the combined model of miR-432-5p + miR-449a and miR-34a-5p + miR-432-5p + miR-449a have better prediction performances. Conclusions The study concludes that the two miRNAs combinations have the potential to be used as biomarkers for schizophrenia diagnoses. The finding may be conducive to overcoming the dilemmas faced by current schizophrenia diagnosis.http://link.springer.com/article/10.1186/s41065-019-0099-3SchizophreniaMiRNAsBiomarkersIdentificationSerum |
| spellingShingle | Kuanjun He Chuang Guo Meng Guo Shuping Tong Qiuli Zhang Hongjun Sun Lin He Yongyong Shi Identification of serum microRNAs as diagnostic biomarkers for schizophrenia Hereditas Schizophrenia MiRNAs Biomarkers Identification Serum |
| title | Identification of serum microRNAs as diagnostic biomarkers for schizophrenia |
| title_full | Identification of serum microRNAs as diagnostic biomarkers for schizophrenia |
| title_fullStr | Identification of serum microRNAs as diagnostic biomarkers for schizophrenia |
| title_full_unstemmed | Identification of serum microRNAs as diagnostic biomarkers for schizophrenia |
| title_short | Identification of serum microRNAs as diagnostic biomarkers for schizophrenia |
| title_sort | identification of serum micrornas as diagnostic biomarkers for schizophrenia |
| topic | Schizophrenia MiRNAs Biomarkers Identification Serum |
| url | http://link.springer.com/article/10.1186/s41065-019-0099-3 |
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