Calcitonin measurement in fine-needle aspirate washout fluid by electrochemiluminescence immunoassay for thyroid tumors
Abstract Purpose For the differential diagnosis of medullary thyroid carcinoma (MTC) on thyroid nodules, ultrasound-guided fine-needle aspiration cytology is a useful and safe procedure, but its diagnostic accuracy is not high enough. As an ancillary method to accurately diagnose MTC, the calcitonin...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | Thyroid Research |
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| Online Access: | http://link.springer.com/article/10.1186/s13044-018-0059-4 |
| _version_ | 1819146179695345664 |
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| author | Minoru Kihara Mitsuyoshi Hirokawa Takumi Kudo Toshitetsu Hayashi Masatoshi Yamamoto Hiroo Masuoka Takuya Higashiyama Mitsuhiro Fukushima Yasuhiro Ito Akihiro Miya Akira Miyauchi |
| author_facet | Minoru Kihara Mitsuyoshi Hirokawa Takumi Kudo Toshitetsu Hayashi Masatoshi Yamamoto Hiroo Masuoka Takuya Higashiyama Mitsuhiro Fukushima Yasuhiro Ito Akihiro Miya Akira Miyauchi |
| author_sort | Minoru Kihara |
| collection | DOAJ |
| description | Abstract Purpose For the differential diagnosis of medullary thyroid carcinoma (MTC) on thyroid nodules, ultrasound-guided fine-needle aspiration cytology is a useful and safe procedure, but its diagnostic accuracy is not high enough. As an ancillary method to accurately diagnose MTC, the calcitonin in fine-needle aspirate washout fluid (FNA-Ct) is used. However, no data are available about cut-off values of FNA-Ct using the currently available electrochemiluminescence immunoassay (ECLIA). Methods We investigated 180 thyroid nodules in 141 patients. After smearing, the syringe and needle used for the FNA were rinsed with normal saline (0.5 mL). The calcitonin in the washout was measured by ECLIA. Results The FNA-Ct in the non-MTC nodules of MTC patients, non-MTC nodules of non-MTC patients, and MTC nodules were 10.6–2100 pg/mL (median 24.6 pg/mL), < 0.5–21.0 pg/mL (median < 0.5 pg/mL), and 94.9–4,070,000 pg/mL (median 177,000 pg/mL), respectively. A receiver operating characteristic analysis of the MTC nodules and the non-MTC nodules of the non-MTC patients indicated that the cut-off value was 21.0 pg/mL, leading to 100% sensitivity and 100% specificity. Conclusions This is the first study to determine the cut-off value of FNA-Ct with an ECLIA, and we propose that the optimal cut-off value is 21.0 pg/mL. |
| first_indexed | 2024-12-22T13:09:49Z |
| format | Article |
| id | doaj.art-58c64403e4f548d08c2305d14c087de5 |
| institution | Directory Open Access Journal |
| issn | 1756-6614 |
| language | English |
| last_indexed | 2024-12-22T13:09:49Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Thyroid Research |
| spelling | doaj.art-58c64403e4f548d08c2305d14c087de52022-12-21T18:24:47ZengBMCThyroid Research1756-66142018-10-011111610.1186/s13044-018-0059-4Calcitonin measurement in fine-needle aspirate washout fluid by electrochemiluminescence immunoassay for thyroid tumorsMinoru Kihara0Mitsuyoshi Hirokawa1Takumi Kudo2Toshitetsu Hayashi3Masatoshi Yamamoto4Hiroo Masuoka5Takuya Higashiyama6Mitsuhiro Fukushima7Yasuhiro Ito8Akihiro Miya9Akira Miyauchi10Departments of Surgery, Kuma HospitalDepartments of Diagnostic Pathology, Kuma HospitalDepartments of Internal Medicine, Kuma HospitalDepartments of Diagnostic Pathology, Kuma HospitalDepartments of Surgery, Kuma HospitalDepartments of Surgery, Kuma HospitalDepartments of Surgery, Kuma HospitalDepartments of Surgery, Kuma HospitalDepartments of Surgery, Kuma HospitalDepartments of Surgery, Kuma HospitalDepartments of Surgery, Kuma HospitalAbstract Purpose For the differential diagnosis of medullary thyroid carcinoma (MTC) on thyroid nodules, ultrasound-guided fine-needle aspiration cytology is a useful and safe procedure, but its diagnostic accuracy is not high enough. As an ancillary method to accurately diagnose MTC, the calcitonin in fine-needle aspirate washout fluid (FNA-Ct) is used. However, no data are available about cut-off values of FNA-Ct using the currently available electrochemiluminescence immunoassay (ECLIA). Methods We investigated 180 thyroid nodules in 141 patients. After smearing, the syringe and needle used for the FNA were rinsed with normal saline (0.5 mL). The calcitonin in the washout was measured by ECLIA. Results The FNA-Ct in the non-MTC nodules of MTC patients, non-MTC nodules of non-MTC patients, and MTC nodules were 10.6–2100 pg/mL (median 24.6 pg/mL), < 0.5–21.0 pg/mL (median < 0.5 pg/mL), and 94.9–4,070,000 pg/mL (median 177,000 pg/mL), respectively. A receiver operating characteristic analysis of the MTC nodules and the non-MTC nodules of the non-MTC patients indicated that the cut-off value was 21.0 pg/mL, leading to 100% sensitivity and 100% specificity. Conclusions This is the first study to determine the cut-off value of FNA-Ct with an ECLIA, and we propose that the optimal cut-off value is 21.0 pg/mL.http://link.springer.com/article/10.1186/s13044-018-0059-4CalcitoninFine-needle aspiration washout fluidMedullary thyroid carcinomaElectrochemiluminescence immunoassayECLIA |
| spellingShingle | Minoru Kihara Mitsuyoshi Hirokawa Takumi Kudo Toshitetsu Hayashi Masatoshi Yamamoto Hiroo Masuoka Takuya Higashiyama Mitsuhiro Fukushima Yasuhiro Ito Akihiro Miya Akira Miyauchi Calcitonin measurement in fine-needle aspirate washout fluid by electrochemiluminescence immunoassay for thyroid tumors Thyroid Research Calcitonin Fine-needle aspiration washout fluid Medullary thyroid carcinoma Electrochemiluminescence immunoassay ECLIA |
| title | Calcitonin measurement in fine-needle aspirate washout fluid by electrochemiluminescence immunoassay for thyroid tumors |
| title_full | Calcitonin measurement in fine-needle aspirate washout fluid by electrochemiluminescence immunoassay for thyroid tumors |
| title_fullStr | Calcitonin measurement in fine-needle aspirate washout fluid by electrochemiluminescence immunoassay for thyroid tumors |
| title_full_unstemmed | Calcitonin measurement in fine-needle aspirate washout fluid by electrochemiluminescence immunoassay for thyroid tumors |
| title_short | Calcitonin measurement in fine-needle aspirate washout fluid by electrochemiluminescence immunoassay for thyroid tumors |
| title_sort | calcitonin measurement in fine needle aspirate washout fluid by electrochemiluminescence immunoassay for thyroid tumors |
| topic | Calcitonin Fine-needle aspiration washout fluid Medullary thyroid carcinoma Electrochemiluminescence immunoassay ECLIA |
| url | http://link.springer.com/article/10.1186/s13044-018-0059-4 |
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