Hemocultures for the parasitological diagnosis of human chronic Chagas' disease
With the purpose of standardization of an hemoculture technique presenting a higher positive rate in the parasitological diagnosis of chronic Chagas' disease in patients with reactive serology (IFT, HA, CFT) the following schedule was used. Thirty ml of venous blood was collected with heparin a...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
1989-03-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821989000100004&lng=en&tlng=en |
| _version_ | 1818215807236177920 |
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| author | Egler Chiari João Carlos Pinto Dias Marta Lana Clea Andrade Chiari |
| author_facet | Egler Chiari João Carlos Pinto Dias Marta Lana Clea Andrade Chiari |
| author_sort | Egler Chiari |
| collection | DOAJ |
| description | With the purpose of standardization of an hemoculture technique presenting a higher positive rate in the parasitological diagnosis of chronic Chagas' disease in patients with reactive serology (IFT, HA, CFT) the following schedule was used. Thirty ml of venous blood was collected with heparin and the plasma was separated by centrifugation (2.000 rpm/30'). The packed cells were washed with LIT medium or PBS which was then removed by centrifugation (2.000 rpm/15'). This material was sampled in 6 screw-tubes 18x200 with 6 ml of LIT medium and incubated at 28°C. These incubated cultures at 28°C were examined after 15, 30, 45 and 60 days. When the hemoculture was not immediately processed after blood collection, the plasma was removed and the sediment enriched with LIT medium and preserved at 4°C. The Xenodiagnosis was performed according to Schenones method used here as a reference technique. Among the various groups of patients examined by both techniques the best results obtained were: 55.08% ofpositivity for hemocultures against 27.5% forxenodiagnosis (X² = 4.54, p = 0.05), with a tubepositivity of 26.6%. Recommendation for screening trials of drug assays is the repetition of method on a same patient 2 or more times in different occasions, as used in xenodiagnosis. |
| first_indexed | 2024-12-12T06:41:57Z |
| format | Article |
| id | doaj.art-58c7488352aa4f5e9f211fc83ee619a6 |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-12-12T06:41:57Z |
| publishDate | 1989-03-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-58c7488352aa4f5e9f211fc83ee619a62022-12-22T00:34:18ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98491989-03-01221192310.1590/S0037-86821989000100004S0037-86821989000100004Hemocultures for the parasitological diagnosis of human chronic Chagas' diseaseEgler Chiari0João Carlos Pinto Dias1Marta Lana2Clea Andrade Chiari3Universidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Ouro PretoUniversidade Federal de Minas GeraisWith the purpose of standardization of an hemoculture technique presenting a higher positive rate in the parasitological diagnosis of chronic Chagas' disease in patients with reactive serology (IFT, HA, CFT) the following schedule was used. Thirty ml of venous blood was collected with heparin and the plasma was separated by centrifugation (2.000 rpm/30'). The packed cells were washed with LIT medium or PBS which was then removed by centrifugation (2.000 rpm/15'). This material was sampled in 6 screw-tubes 18x200 with 6 ml of LIT medium and incubated at 28°C. These incubated cultures at 28°C were examined after 15, 30, 45 and 60 days. When the hemoculture was not immediately processed after blood collection, the plasma was removed and the sediment enriched with LIT medium and preserved at 4°C. The Xenodiagnosis was performed according to Schenones method used here as a reference technique. Among the various groups of patients examined by both techniques the best results obtained were: 55.08% ofpositivity for hemocultures against 27.5% forxenodiagnosis (X² = 4.54, p = 0.05), with a tubepositivity of 26.6%. Recommendation for screening trials of drug assays is the repetition of method on a same patient 2 or more times in different occasions, as used in xenodiagnosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821989000100004&lng=en&tlng=enDoença de ChagasDiagnóstico parasitológicoHemocultura e xenodiagnósticoTrypanosoma cruzi |
| spellingShingle | Egler Chiari João Carlos Pinto Dias Marta Lana Clea Andrade Chiari Hemocultures for the parasitological diagnosis of human chronic Chagas' disease Revista da Sociedade Brasileira de Medicina Tropical Doença de Chagas Diagnóstico parasitológico Hemocultura e xenodiagnóstico Trypanosoma cruzi |
| title | Hemocultures for the parasitological diagnosis of human chronic Chagas' disease |
| title_full | Hemocultures for the parasitological diagnosis of human chronic Chagas' disease |
| title_fullStr | Hemocultures for the parasitological diagnosis of human chronic Chagas' disease |
| title_full_unstemmed | Hemocultures for the parasitological diagnosis of human chronic Chagas' disease |
| title_short | Hemocultures for the parasitological diagnosis of human chronic Chagas' disease |
| title_sort | hemocultures for the parasitological diagnosis of human chronic chagas disease |
| topic | Doença de Chagas Diagnóstico parasitológico Hemocultura e xenodiagnóstico Trypanosoma cruzi |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821989000100004&lng=en&tlng=en |
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