Impairment of Circulating CD4+CD25+GARP+ Regulatory T Cells in Patients with Acute Coronary Syndrome
Background: Atherosclerosis (AS) is an inflammatory and immune disease. Regulatory T cells (Tregs) suppress the activation of T cells and have been shown to play a protective role during the pathogenesis of AS. However, specific markers for Tregs are lacking. Recently, glycoprotein A repetitions pre...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2014-02-01
|
| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/358639 |
| _version_ | 1818177311093030912 |
|---|---|
| author | Kai Meng Wei Zhang Yucheng Zhong Xiaobo Mao Yingzhong Lin Ying Huang Mingjian Lang Yudong Peng Zhengfeng Zhu Yuzhou Liu Xiaoqi Zhao Kunwu Yu Bangwei Wu Qingwei Ji Qiutang Zeng |
| author_facet | Kai Meng Wei Zhang Yucheng Zhong Xiaobo Mao Yingzhong Lin Ying Huang Mingjian Lang Yudong Peng Zhengfeng Zhu Yuzhou Liu Xiaoqi Zhao Kunwu Yu Bangwei Wu Qingwei Ji Qiutang Zeng |
| author_sort | Kai Meng |
| collection | DOAJ |
| description | Background: Atherosclerosis (AS) is an inflammatory and immune disease. Regulatory T cells (Tregs) suppress the activation of T cells and have been shown to play a protective role during the pathogenesis of AS. However, specific markers for Tregs are lacking. Recently, glycoprotein A repetitions predominant (GARP) was discovered as a specific marker of activated Tregs, and we therefore utilized GARP as a specific surface marker for Tregs in the current study. Methods: To assess whether GARP+ Tregs are downregulated in patients with acute coronary syndrome (ACS), we examined CD4+CD25+GARP+ T cell frequencies as well as their associated cytokines and suppressive function. Additionally, we compared GARP expression to that of FOXP3, which may be more sensitive as a marker of activated Tregs in patients with ACS. Results: Patients with ACS demonstrated a significant decrease in circulating CD4+CD25+GARP+ Tregs. Moreover, the suppressive function of Tregs and levels of related cytokines were also impaired in ACS patients compared to those with stable angina (SA) or normal coronary artery (NCA). Additionally, after TCR stimulation, peripheral blood mononuclear cells (PBMCs) from patients with ACS exhibited a decrease in CD4+CD25+GARP+ Tregs. Conclusions: These fnding indicate that circulating CD4+CD25+GARP+ Tregs are impaired in patients withACS. Thus, targeting GARP may promote the protective function of Tregs in ACS. |
| first_indexed | 2024-12-11T20:30:04Z |
| format | Article |
| id | doaj.art-58c7a1cc67b0406c92ddb589e0c077f2 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-11T20:30:04Z |
| publishDate | 2014-02-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-58c7a1cc67b0406c92ddb589e0c077f22022-12-22T00:51:51ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782014-02-0133362163210.1159/000358639358639Impairment of Circulating CD4+CD25+GARP+ Regulatory T Cells in Patients with Acute Coronary SyndromeKai MengWei ZhangYucheng ZhongXiaobo MaoYingzhong LinYing HuangMingjian LangYudong PengZhengfeng ZhuYuzhou LiuXiaoqi ZhaoKunwu YuBangwei WuQingwei JiQiutang ZengBackground: Atherosclerosis (AS) is an inflammatory and immune disease. Regulatory T cells (Tregs) suppress the activation of T cells and have been shown to play a protective role during the pathogenesis of AS. However, specific markers for Tregs are lacking. Recently, glycoprotein A repetitions predominant (GARP) was discovered as a specific marker of activated Tregs, and we therefore utilized GARP as a specific surface marker for Tregs in the current study. Methods: To assess whether GARP+ Tregs are downregulated in patients with acute coronary syndrome (ACS), we examined CD4+CD25+GARP+ T cell frequencies as well as their associated cytokines and suppressive function. Additionally, we compared GARP expression to that of FOXP3, which may be more sensitive as a marker of activated Tregs in patients with ACS. Results: Patients with ACS demonstrated a significant decrease in circulating CD4+CD25+GARP+ Tregs. Moreover, the suppressive function of Tregs and levels of related cytokines were also impaired in ACS patients compared to those with stable angina (SA) or normal coronary artery (NCA). Additionally, after TCR stimulation, peripheral blood mononuclear cells (PBMCs) from patients with ACS exhibited a decrease in CD4+CD25+GARP+ Tregs. Conclusions: These fnding indicate that circulating CD4+CD25+GARP+ Tregs are impaired in patients withACS. Thus, targeting GARP may promote the protective function of Tregs in ACS.http://www.karger.com/Article/FullText/358639AtherosclerosisRegulatory T cellsGARPImmune systemAcute coronary syndrome |
| spellingShingle | Kai Meng Wei Zhang Yucheng Zhong Xiaobo Mao Yingzhong Lin Ying Huang Mingjian Lang Yudong Peng Zhengfeng Zhu Yuzhou Liu Xiaoqi Zhao Kunwu Yu Bangwei Wu Qingwei Ji Qiutang Zeng Impairment of Circulating CD4+CD25+GARP+ Regulatory T Cells in Patients with Acute Coronary Syndrome Cellular Physiology and Biochemistry Atherosclerosis Regulatory T cells GARP Immune system Acute coronary syndrome |
| title | Impairment of Circulating CD4+CD25+GARP+ Regulatory T Cells in Patients with Acute Coronary Syndrome |
| title_full | Impairment of Circulating CD4+CD25+GARP+ Regulatory T Cells in Patients with Acute Coronary Syndrome |
| title_fullStr | Impairment of Circulating CD4+CD25+GARP+ Regulatory T Cells in Patients with Acute Coronary Syndrome |
| title_full_unstemmed | Impairment of Circulating CD4+CD25+GARP+ Regulatory T Cells in Patients with Acute Coronary Syndrome |
| title_short | Impairment of Circulating CD4+CD25+GARP+ Regulatory T Cells in Patients with Acute Coronary Syndrome |
| title_sort | impairment of circulating cd4 cd25 garp regulatory t cells in patients with acute coronary syndrome |
| topic | Atherosclerosis Regulatory T cells GARP Immune system Acute coronary syndrome |
| url | http://www.karger.com/Article/FullText/358639 |
| work_keys_str_mv | AT kaimeng impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT weizhang impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT yuchengzhong impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT xiaobomao impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT yingzhonglin impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT yinghuang impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT mingjianlang impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT yudongpeng impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT zhengfengzhu impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT yuzhouliu impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT xiaoqizhao impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT kunwuyu impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT bangweiwu impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT qingweiji impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome AT qiutangzeng impairmentofcirculatingcd4cd25garpregulatorytcellsinpatientswithacutecoronarysyndrome |