Chemical Composition and <em>in Vitro</em> Evaluation of the Antioxidant and Antimicrobial Activities of <em>Eucalyptus gillii </em>Essential Oil and Extracts<em></em>
In this study, essential oil and various extracts (hexane, petroleum ether, acetone, ethanol, methanol and water) of <em>Eucalyptus gilii</em> were screened for their chemical composition, antimicrobial and antioxidant activities. The essential oil chemical composition wa...
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MDPI AG
2012-08-01
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| Series: | Molecules |
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| author | François Couderc Jalloul Bouajila Florence Mathieu Yan Yvon Ahmed Lebrihi Dorsaf Ben Hassine Manef Abderrabba |
| author_facet | François Couderc Jalloul Bouajila Florence Mathieu Yan Yvon Ahmed Lebrihi Dorsaf Ben Hassine Manef Abderrabba |
| author_sort | François Couderc |
| collection | DOAJ |
| description | In this study, essential oil and various extracts (hexane, petroleum ether, acetone, ethanol, methanol and water) of <em>Eucalyptus gilii</em> were screened for their chemical composition, antimicrobial and antioxidant activities. The essential oil chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID), respectively. Thirty four compounds were identified, corresponding to 99.5% of the total essential oil. Tannins [104.9–251.3 g catechin equivalent (CE)/Kg dry mass], flavonoids [3.3–34.3 g quercetin equivalent (QE)/Kg dry mass], phenolics [4.7–216.6 g gallic acid equivalent (GAE)/Kg dry mass] and anthocyannins [1.2–45.3 mg cyanidin-3-glucoside equivalent (C3GE)/Kg dry mass] of various extracts were investigated. Free radical scavenging capacity of all samples was determinedt. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, the IC<sub>50</sub> of essential oil was 163.5 ± 10.7 mg/L and in the 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS) assay, it was 94.7 ± 7.1 mg/L. Among the various extracts, the water extract showed the best result (IC<sub>50</sub> = 11.4 ± 0.6 mg/L) in the DPPH assay which was comparable to vitamin C (IC<sub>50</sub> = 4.4 ± 0.2 mg/L). The antimicrobial activities were evaluated against different bacterial and fungal strains. Gram positive bacteria were found to be more sensitive to the essential oil and extracts than Gram negative ones. Anthocyanins seem to have a major effect on the growth of <em>Bacillus subtilis</em> (R<sup>2</sup> = 0.79). A significant antifungal activity was observed against the yeast and fungi. Correlations between chemical composition and antioxidant activities were studied and R<sup>2</sup> values were about 0.96 for the effect of phenolics on the DPPH assay. |
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| spelling | doaj.art-58c946e6e0b24024bda6fe0fb8e7e6a82022-12-21T18:51:42ZengMDPI AGMolecules1420-30492012-08-011789540955810.3390/molecules17089540Chemical Composition and <em>in Vitro</em> Evaluation of the Antioxidant and Antimicrobial Activities of <em>Eucalyptus gillii </em>Essential Oil and Extracts<em></em>François CoudercJalloul BouajilaFlorence MathieuYan YvonAhmed LebrihiDorsaf Ben HassineManef AbderrabbaIn this study, essential oil and various extracts (hexane, petroleum ether, acetone, ethanol, methanol and water) of <em>Eucalyptus gilii</em> were screened for their chemical composition, antimicrobial and antioxidant activities. The essential oil chemical composition was analyzed by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC-FID), respectively. Thirty four compounds were identified, corresponding to 99.5% of the total essential oil. Tannins [104.9–251.3 g catechin equivalent (CE)/Kg dry mass], flavonoids [3.3–34.3 g quercetin equivalent (QE)/Kg dry mass], phenolics [4.7–216.6 g gallic acid equivalent (GAE)/Kg dry mass] and anthocyannins [1.2–45.3 mg cyanidin-3-glucoside equivalent (C3GE)/Kg dry mass] of various extracts were investigated. Free radical scavenging capacity of all samples was determinedt. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, the IC<sub>50</sub> of essential oil was 163.5 ± 10.7 mg/L and in the 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS) assay, it was 94.7 ± 7.1 mg/L. Among the various extracts, the water extract showed the best result (IC<sub>50</sub> = 11.4 ± 0.6 mg/L) in the DPPH assay which was comparable to vitamin C (IC<sub>50</sub> = 4.4 ± 0.2 mg/L). The antimicrobial activities were evaluated against different bacterial and fungal strains. Gram positive bacteria were found to be more sensitive to the essential oil and extracts than Gram negative ones. Anthocyanins seem to have a major effect on the growth of <em>Bacillus subtilis</em> (R<sup>2</sup> = 0.79). A significant antifungal activity was observed against the yeast and fungi. Correlations between chemical composition and antioxidant activities were studied and R<sup>2</sup> values were about 0.96 for the effect of phenolics on the DPPH assay.http://www.mdpi.com/1420-3049/17/8/9540<em>Eucalyptus gillii</em>essential oilextractsGC-MSantioxidants activityDPPHABTSantimicrobial activity |
| spellingShingle | François Couderc Jalloul Bouajila Florence Mathieu Yan Yvon Ahmed Lebrihi Dorsaf Ben Hassine Manef Abderrabba Chemical Composition and <em>in Vitro</em> Evaluation of the Antioxidant and Antimicrobial Activities of <em>Eucalyptus gillii </em>Essential Oil and Extracts<em></em> Molecules <em>Eucalyptus gillii</em> essential oil extracts GC-MS antioxidants activity DPPH ABTS antimicrobial activity |
| title | Chemical Composition and <em>in Vitro</em> Evaluation of the Antioxidant and Antimicrobial Activities of <em>Eucalyptus gillii </em>Essential Oil and Extracts<em></em> |
| title_full | Chemical Composition and <em>in Vitro</em> Evaluation of the Antioxidant and Antimicrobial Activities of <em>Eucalyptus gillii </em>Essential Oil and Extracts<em></em> |
| title_fullStr | Chemical Composition and <em>in Vitro</em> Evaluation of the Antioxidant and Antimicrobial Activities of <em>Eucalyptus gillii </em>Essential Oil and Extracts<em></em> |
| title_full_unstemmed | Chemical Composition and <em>in Vitro</em> Evaluation of the Antioxidant and Antimicrobial Activities of <em>Eucalyptus gillii </em>Essential Oil and Extracts<em></em> |
| title_short | Chemical Composition and <em>in Vitro</em> Evaluation of the Antioxidant and Antimicrobial Activities of <em>Eucalyptus gillii </em>Essential Oil and Extracts<em></em> |
| title_sort | chemical composition and lt em gt in vitro lt em gt evaluation of the antioxidant and antimicrobial activities of lt em gt eucalyptus gillii lt em gt essential oil and extracts lt em gt lt em gt |
| topic | <em>Eucalyptus gillii</em> essential oil extracts GC-MS antioxidants activity DPPH ABTS antimicrobial activity |
| url | http://www.mdpi.com/1420-3049/17/8/9540 |
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