Optimization of Culture Condition for the Production of Menaquinone-7 by Bacillus Subtilis Natto
Background: Vitamin K2 refers to a series of naphthoquinone derivatives, which have a variety of physiological and pharmacological functions for the human body. The most important type of vitamin K2 is menaquinone-7 (MK-7), an expensive raw material with no local manufacturers in Iran. Objectives: S...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences and Health Services
2022-03-01
|
| Series: | Research in Molecular Medicine |
| Subjects: | |
| Online Access: | http://rmm.mazums.ac.ir/article-1-454-en.pdf |
| _version_ | 1797799825628987392 |
|---|---|
| author | Mohammad Sharifzadeh Negar Mottaghi-Dastjerdi Mohammad Soltany-Rezaee-Rad Marjan Shariatpanahi Israa Khalid AL-Yasari Sara Noroozi Eshlaghi |
| author_facet | Mohammad Sharifzadeh Negar Mottaghi-Dastjerdi Mohammad Soltany-Rezaee-Rad Marjan Shariatpanahi Israa Khalid AL-Yasari Sara Noroozi Eshlaghi |
| author_sort | Mohammad Sharifzadeh |
| collection | DOAJ |
| description | Background: Vitamin K2 refers to a series of naphthoquinone derivatives, which have a variety of physiological and pharmacological functions for the human body. The most important type of vitamin K2 is menaquinone-7 (MK-7), an expensive raw material with no local manufacturers in Iran.
Objectives: Since there was no report on the yield of MK-7 produced by the currently available Bacillus subtilis natto species in Iran, this study aims to optimize the culture condition for the production of MK-7 using this Bacillus species.
Materials and Methods: The base medium (BM) for MK-7 production contained glycerol (6.3%), soybean peptone (3%), and yeast extract (0.51%). The selected factors for optimizing the MK-7 production included the incubation temperature (30, 37, and 40°C) and incubation time (72, 96, and 120hr) with/without the addition of K2HPO4 to the fermentation medium. Three sets of experiments with six modes in each set were designed based on these parameters. MK-7 content was analyzed by the HPLC method.
Results: Two experiments showed the highest MK-7 production yields of 0.319 and 0.3158 mg/L. The culture condition for both of these yields was as follows:120 hours incubation time in the presence of K2HPO4. However, the incubation temperature was different in these two experiments. The incubation temperature of 30°C resulted in 0.319 mg/L MK-7 concentration, and 37°C yielded 0.3158 mg/L.
Conclusion: B. subtilis natto (IBRC-M 11153) is suitable to be used as a basic platform for the mutation and production of a high-producer species. Optimizing the culture conditions using the wild-type species is not beneficial in increasing the production ability of the bacterium. It is necessary to use different methods for enhancing the production yield of MK-7 to lower the cost of microbial production and make the industrial process economic. |
| first_indexed | 2024-03-13T04:24:47Z |
| format | Article |
| id | doaj.art-58cccefc2d174258a5adfb0585ccc6bf |
| institution | Directory Open Access Journal |
| issn | 2322-1348 2322-133X |
| language | English |
| last_indexed | 2024-03-13T04:24:47Z |
| publishDate | 2022-03-01 |
| publisher | Mazandaran University of Medical Sciences and Health Services |
| record_format | Article |
| series | Research in Molecular Medicine |
| spelling | doaj.art-58cccefc2d174258a5adfb0585ccc6bf2023-06-20T06:22:16ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482322-133X2022-03-011013746Optimization of Culture Condition for the Production of Menaquinone-7 by Bacillus Subtilis NattoMohammad Sharifzadeh0Negar Mottaghi-Dastjerdi1Mohammad Soltany-Rezaee-Rad2Marjan Shariatpanahi3Israa Khalid AL-Yasari4Sara Noroozi Eshlaghi5 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Iran University of Medical Sciences, Tehran, Iran Department of Pharmaceutical Biotechnology and Pharmaceutical Biotechnology Research Center, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Department of Pharmacology and Toxicology, School of Pharmacy, Iran University of Medical Sciences, Tehran, Iran Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Iran University of Medical Sciences, Tehran, Iran Department of Pharmacology and Toxicology, School of Pharmacy, Iran University of Medical Sciences, Tehran, Iran. Department of Chemistry, Faculty of Science, University of Zanjan, Zanjan, Iran. Background: Vitamin K2 refers to a series of naphthoquinone derivatives, which have a variety of physiological and pharmacological functions for the human body. The most important type of vitamin K2 is menaquinone-7 (MK-7), an expensive raw material with no local manufacturers in Iran. Objectives: Since there was no report on the yield of MK-7 produced by the currently available Bacillus subtilis natto species in Iran, this study aims to optimize the culture condition for the production of MK-7 using this Bacillus species. Materials and Methods: The base medium (BM) for MK-7 production contained glycerol (6.3%), soybean peptone (3%), and yeast extract (0.51%). The selected factors for optimizing the MK-7 production included the incubation temperature (30, 37, and 40°C) and incubation time (72, 96, and 120hr) with/without the addition of K2HPO4 to the fermentation medium. Three sets of experiments with six modes in each set were designed based on these parameters. MK-7 content was analyzed by the HPLC method. Results: Two experiments showed the highest MK-7 production yields of 0.319 and 0.3158 mg/L. The culture condition for both of these yields was as follows:120 hours incubation time in the presence of K2HPO4. However, the incubation temperature was different in these two experiments. The incubation temperature of 30°C resulted in 0.319 mg/L MK-7 concentration, and 37°C yielded 0.3158 mg/L. Conclusion: B. subtilis natto (IBRC-M 11153) is suitable to be used as a basic platform for the mutation and production of a high-producer species. Optimizing the culture conditions using the wild-type species is not beneficial in increasing the production ability of the bacterium. It is necessary to use different methods for enhancing the production yield of MK-7 to lower the cost of microbial production and make the industrial process economic.http://rmm.mazums.ac.ir/article-1-454-en.pdfvitamin k2bacillus subtilis nattomenaquinone-7fermentation |
| spellingShingle | Mohammad Sharifzadeh Negar Mottaghi-Dastjerdi Mohammad Soltany-Rezaee-Rad Marjan Shariatpanahi Israa Khalid AL-Yasari Sara Noroozi Eshlaghi Optimization of Culture Condition for the Production of Menaquinone-7 by Bacillus Subtilis Natto Research in Molecular Medicine vitamin k2 bacillus subtilis natto menaquinone-7 fermentation |
| title | Optimization of Culture Condition for the Production of Menaquinone-7 by Bacillus Subtilis Natto |
| title_full | Optimization of Culture Condition for the Production of Menaquinone-7 by Bacillus Subtilis Natto |
| title_fullStr | Optimization of Culture Condition for the Production of Menaquinone-7 by Bacillus Subtilis Natto |
| title_full_unstemmed | Optimization of Culture Condition for the Production of Menaquinone-7 by Bacillus Subtilis Natto |
| title_short | Optimization of Culture Condition for the Production of Menaquinone-7 by Bacillus Subtilis Natto |
| title_sort | optimization of culture condition for the production of menaquinone 7 by bacillus subtilis natto |
| topic | vitamin k2 bacillus subtilis natto menaquinone-7 fermentation |
| url | http://rmm.mazums.ac.ir/article-1-454-en.pdf |
| work_keys_str_mv | AT mohammadsharifzadeh optimizationofcultureconditionfortheproductionofmenaquinone7bybacillussubtilisnatto AT negarmottaghidastjerdi optimizationofcultureconditionfortheproductionofmenaquinone7bybacillussubtilisnatto AT mohammadsoltanyrezaeerad optimizationofcultureconditionfortheproductionofmenaquinone7bybacillussubtilisnatto AT marjanshariatpanahi optimizationofcultureconditionfortheproductionofmenaquinone7bybacillussubtilisnatto AT israakhalidalyasari optimizationofcultureconditionfortheproductionofmenaquinone7bybacillussubtilisnatto AT saranoroozieshlaghi optimizationofcultureconditionfortheproductionofmenaquinone7bybacillussubtilisnatto |