VEGF, HIF-1α and PEDF expression in the retina of streptozotocin-induced diabetics rats treated with ozone
AIM:To study vascular endothelial growth factor(VEGF), pigment epithelium derived factor(PEDF)and hypoxia inducible factor-1α(HIF-1α)expression in retinal and serum in ozone-treated streptozotocin(STZ)-induced diabetic rats and to determine the possible efficacy of ozone therapy for diabetic retinop...
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Press of International Journal of Ophthalmology (IJO PRESS)
2016-02-01
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Series: | Guoji Yanke Zazhi |
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Online Access: | http://ies.ijo.cn/cn_publish/2016/2/201602001.pdf |
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author | Ting-Yu Xie Cui-Li Zhang Xue-Yi Chen |
author_facet | Ting-Yu Xie Cui-Li Zhang Xue-Yi Chen |
author_sort | Ting-Yu Xie |
collection | DOAJ |
description | AIM:To study vascular endothelial growth factor(VEGF), pigment epithelium derived factor(PEDF)and hypoxia inducible factor-1α(HIF-1α)expression in retinal and serum in ozone-treated streptozotocin(STZ)-induced diabetic rats and to determine the possible efficacy of ozone therapy for diabetic retinopathy(DR).<p>METHODS:Seventy male Sprague-Dawley rats were used. Group A(<i>n</i>=10)received a normal diet, diabetic molding established by intraperitoneal injection of STZ(50mg/ml), then divided into three groups, group B without any intervene; and groups C and D given oxygen and ozone clyster treatment respectively, twice per week for 1mo. Retina and blood were taken under general anesthesia. Reverse transcription-polymerase chain reaction(RT-PCR)and enzyme-linked immunosorbent assay(ELISA)methods were used to study retinal and serum VEGF, HIF-1α and PEDF expression.<p>RESULTS:VEGF occurred mostly in the inner layer of the retina; the difference of VEGF in the retina among each group has statistical difference(<i>F</i>=23.923; <i>P</i>=0.000); in which, group D closer to group A, but still has statistical difference(<i>P</i><0.05); there is no difference between group B and C(<i>P</i>>0.05)except for no difference between group A and D(<i>P</i>>0.05), others as same result as retinal VEGF expression.HIF-1α expression decreased in ozone-treated rats(group D)compared with control group(<i>P</i><0.05); the difference between group A and groups B and C was significant(<i>P</i><0.05), and the difference between groups B and C with no statistical difference(<i>P</i>>0.05). Overall, PEDF expression was lower than VEGF and HIF-1α, and groups A and D showed more expression than groups B and C, but the differences were not significant(<i>P</i> >0.05).<p>CONCLUSION: Ozone administration can reduce the VEGF and HIF-1α expression and ozone may have potential uses in its treatment. |
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id | doaj.art-58ce7a1fc4f740389ce9f4d2bc2f65ad |
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issn | 1672-5123 1672-5123 |
language | English |
last_indexed | 2024-04-11T23:00:30Z |
publishDate | 2016-02-01 |
publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
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series | Guoji Yanke Zazhi |
spelling | doaj.art-58ce7a1fc4f740389ce9f4d2bc2f65ad2022-12-22T03:58:11ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232016-02-0116219520010.3980/j.issn.1672-5123.2016.2.01VEGF, HIF-1α and PEDF expression in the retina of streptozotocin-induced diabetics rats treated with ozoneTing-Yu Xie0Cui-Li Zhang1Xue-Yi Chen2Department of Ophthalmology, the First Affiliated Hospital of Xinjiang Medical University,Urumchi 830011, Xinjiang Uygur Autonomous Region, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Xinjiang Medical University,Urumchi 830011, Xinjiang Uygur Autonomous Region, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Xinjiang Medical University,Urumchi 830011, Xinjiang Uygur Autonomous Region, ChinaAIM:To study vascular endothelial growth factor(VEGF), pigment epithelium derived factor(PEDF)and hypoxia inducible factor-1α(HIF-1α)expression in retinal and serum in ozone-treated streptozotocin(STZ)-induced diabetic rats and to determine the possible efficacy of ozone therapy for diabetic retinopathy(DR).<p>METHODS:Seventy male Sprague-Dawley rats were used. Group A(<i>n</i>=10)received a normal diet, diabetic molding established by intraperitoneal injection of STZ(50mg/ml), then divided into three groups, group B without any intervene; and groups C and D given oxygen and ozone clyster treatment respectively, twice per week for 1mo. Retina and blood were taken under general anesthesia. Reverse transcription-polymerase chain reaction(RT-PCR)and enzyme-linked immunosorbent assay(ELISA)methods were used to study retinal and serum VEGF, HIF-1α and PEDF expression.<p>RESULTS:VEGF occurred mostly in the inner layer of the retina; the difference of VEGF in the retina among each group has statistical difference(<i>F</i>=23.923; <i>P</i>=0.000); in which, group D closer to group A, but still has statistical difference(<i>P</i><0.05); there is no difference between group B and C(<i>P</i>>0.05)except for no difference between group A and D(<i>P</i>>0.05), others as same result as retinal VEGF expression.HIF-1α expression decreased in ozone-treated rats(group D)compared with control group(<i>P</i><0.05); the difference between group A and groups B and C was significant(<i>P</i><0.05), and the difference between groups B and C with no statistical difference(<i>P</i>>0.05). Overall, PEDF expression was lower than VEGF and HIF-1α, and groups A and D showed more expression than groups B and C, but the differences were not significant(<i>P</i> >0.05).<p>CONCLUSION: Ozone administration can reduce the VEGF and HIF-1α expression and ozone may have potential uses in its treatment.http://ies.ijo.cn/cn_publish/2016/2/201602001.pdfdiabetic retinopathyozonevascular endothelial growth factorpigment epithelium derived factorhypoxia inducible factor-1α |
spellingShingle | Ting-Yu Xie Cui-Li Zhang Xue-Yi Chen VEGF, HIF-1α and PEDF expression in the retina of streptozotocin-induced diabetics rats treated with ozone Guoji Yanke Zazhi diabetic retinopathy ozone vascular endothelial growth factor pigment epithelium derived factor hypoxia inducible factor-1α |
title | VEGF, HIF-1α and PEDF expression in the retina of streptozotocin-induced diabetics rats treated with ozone |
title_full | VEGF, HIF-1α and PEDF expression in the retina of streptozotocin-induced diabetics rats treated with ozone |
title_fullStr | VEGF, HIF-1α and PEDF expression in the retina of streptozotocin-induced diabetics rats treated with ozone |
title_full_unstemmed | VEGF, HIF-1α and PEDF expression in the retina of streptozotocin-induced diabetics rats treated with ozone |
title_short | VEGF, HIF-1α and PEDF expression in the retina of streptozotocin-induced diabetics rats treated with ozone |
title_sort | vegf hif 1α and pedf expression in the retina of streptozotocin induced diabetics rats treated with ozone |
topic | diabetic retinopathy ozone vascular endothelial growth factor pigment epithelium derived factor hypoxia inducible factor-1α |
url | http://ies.ijo.cn/cn_publish/2016/2/201602001.pdf |
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