Efficient Confirmation of Plant Viral Proteins and Identification of Specific Viral Strains by nanoLC-ESI-Q-TOF Using Single-Leaf-Tissue Samples

Efficient Confirmation of Plant Viral Proteins and Identification of Specific Viral Strains by nanoLC-ESI-Q-TOF Using Single-Leaf-Tissue Samples

Plant viruses are important pathogens that cause significant crop losses. A plant protein extraction protocol that combines crushing the tissue by a pestle in liquid nitrogen with subsequent crushing by a roller-ball crusher in urea solution, followed by RuBisCO depletion, reduction, alkylation, pro...

Full description

Bibliographic Details
Main Authors: Pavel Cejnar, Štěpánka Kučková, Jiří Šantrůček, Miroslav Glasa, Petr Komínek, Daniel Mihálik, Lucie Slavíková, Leona Leišová-Svobodová, Tatiana Smirnova, Radovan Hynek, Jiban Kumar Kundu, Pavel Ryšánek
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Pathogens
Subjects:
protein extraction protocol
LC-MS/MS
virus detection
viral proteins detection
Online Access:https://www.mdpi.com/2076-0817/9/11/966

Similar Items

Similar Items