Microfluidic-Based Electrochemical Immunosensing of Ferritin
Ferritin is a clinically important biomarker which reflects the state of iron in the body and is directly involved with anemia. Current methods available for ferritin estimation are generally not portable or they do not provide a fast response. To combat these issues, an attempt was made for lab-on-...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/10/8/91 |
| _version_ | 1827711456163921920 |
|---|---|
| author | Mayank Garg Martin Gedsted Christensen Alexander Iles Amit L. Sharma Suman Singh Nicole Pamme |
| author_facet | Mayank Garg Martin Gedsted Christensen Alexander Iles Amit L. Sharma Suman Singh Nicole Pamme |
| author_sort | Mayank Garg |
| collection | DOAJ |
| description | Ferritin is a clinically important biomarker which reflects the state of iron in the body and is directly involved with anemia. Current methods available for ferritin estimation are generally not portable or they do not provide a fast response. To combat these issues, an attempt was made for lab-on-a-chip-based electrochemical detection of ferritin, developed with an integrated electrochemically active screen-printed electrode (SPE), combining nanotechnology, microfluidics, and electrochemistry. The SPE surface was modified with amine-functionalized graphene oxide to facilitate the binding of ferritin antibodies on the electrode surface. The functionalized SPE was embedded in the microfluidic flow cell with a simple magnetic clamping mechanism to allow continuous electrochemical detection of ferritin. Ferritin detection was accomplished via cyclic voltammetry with a dynamic linear range from 7.81 to 500 ng·mL<sup>−1</sup> and an LOD of 0.413 ng·mL<sup>−1</sup>. The sensor performance was verified with spiked human serum samples. Furthermore, the sensor was validated by comparing its response with the response of the conventional ELISA method. The current method of microfluidic flow cell-based electrochemical ferritin detection demonstrated promising sensitivity and selectivity. This confirmed the plausibility of using the reported technique in point-of-care testing applications at a much faster rate than conventional techniques. |
| first_indexed | 2024-03-10T17:54:48Z |
| format | Article |
| id | doaj.art-58d32123ad3b45cab691597a980030f3 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T17:54:48Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-58d32123ad3b45cab691597a980030f32023-11-20T09:13:21ZengMDPI AGBiosensors2079-63742020-08-011089110.3390/bios10080091Microfluidic-Based Electrochemical Immunosensing of FerritinMayank Garg0Martin Gedsted Christensen1Alexander Iles2Amit L. Sharma3Suman Singh4Nicole Pamme5CSIR-Central Scientific Instruments Organisation, Sector 30-C, Chandigarh 160030, IndiaDepartment of Chemistry and Biochemistry, University of Hull, Cottingham Road, Hull HU6 7RX, UKDepartment of Chemistry and Biochemistry, University of Hull, Cottingham Road, Hull HU6 7RX, UKCSIR-Central Scientific Instruments Organisation, Sector 30-C, Chandigarh 160030, IndiaCSIR-Central Scientific Instruments Organisation, Sector 30-C, Chandigarh 160030, IndiaDepartment of Chemistry and Biochemistry, University of Hull, Cottingham Road, Hull HU6 7RX, UKFerritin is a clinically important biomarker which reflects the state of iron in the body and is directly involved with anemia. Current methods available for ferritin estimation are generally not portable or they do not provide a fast response. To combat these issues, an attempt was made for lab-on-a-chip-based electrochemical detection of ferritin, developed with an integrated electrochemically active screen-printed electrode (SPE), combining nanotechnology, microfluidics, and electrochemistry. The SPE surface was modified with amine-functionalized graphene oxide to facilitate the binding of ferritin antibodies on the electrode surface. The functionalized SPE was embedded in the microfluidic flow cell with a simple magnetic clamping mechanism to allow continuous electrochemical detection of ferritin. Ferritin detection was accomplished via cyclic voltammetry with a dynamic linear range from 7.81 to 500 ng·mL<sup>−1</sup> and an LOD of 0.413 ng·mL<sup>−1</sup>. The sensor performance was verified with spiked human serum samples. Furthermore, the sensor was validated by comparing its response with the response of the conventional ELISA method. The current method of microfluidic flow cell-based electrochemical ferritin detection demonstrated promising sensitivity and selectivity. This confirmed the plausibility of using the reported technique in point-of-care testing applications at a much faster rate than conventional techniques.https://www.mdpi.com/2079-6374/10/8/91amine functionalizationgraphene oxideimmunosensorelectrochemistryferritinmicrofluidics |
| spellingShingle | Mayank Garg Martin Gedsted Christensen Alexander Iles Amit L. Sharma Suman Singh Nicole Pamme Microfluidic-Based Electrochemical Immunosensing of Ferritin Biosensors amine functionalization graphene oxide immunosensor electrochemistry ferritin microfluidics |
| title | Microfluidic-Based Electrochemical Immunosensing of Ferritin |
| title_full | Microfluidic-Based Electrochemical Immunosensing of Ferritin |
| title_fullStr | Microfluidic-Based Electrochemical Immunosensing of Ferritin |
| title_full_unstemmed | Microfluidic-Based Electrochemical Immunosensing of Ferritin |
| title_short | Microfluidic-Based Electrochemical Immunosensing of Ferritin |
| title_sort | microfluidic based electrochemical immunosensing of ferritin |
| topic | amine functionalization graphene oxide immunosensor electrochemistry ferritin microfluidics |
| url | https://www.mdpi.com/2079-6374/10/8/91 |
| work_keys_str_mv | AT mayankgarg microfluidicbasedelectrochemicalimmunosensingofferritin AT martingedstedchristensen microfluidicbasedelectrochemicalimmunosensingofferritin AT alexanderiles microfluidicbasedelectrochemicalimmunosensingofferritin AT amitlsharma microfluidicbasedelectrochemicalimmunosensingofferritin AT sumansingh microfluidicbasedelectrochemicalimmunosensingofferritin AT nicolepamme microfluidicbasedelectrochemicalimmunosensingofferritin |