The Complete Mitochondrial Genome of the Foodborne Parasitic Pathogen Cyclospora cayetanensis.
Cyclospora cayetanensis is a human-specific coccidian parasite responsible for several food and water-related outbreaks around the world, including the most recent ones involving over 900 persons in 2013 and 2014 outbreaks in the USA. Multicopy organellar DNA such as mitochondrion genomes have been...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4455993?pdf=render |
| _version_ | 1818152087853203456 |
|---|---|
| author | Hediye Nese Cinar Gopal Gopinath Karen Jarvis Helen R Murphy |
| author_facet | Hediye Nese Cinar Gopal Gopinath Karen Jarvis Helen R Murphy |
| author_sort | Hediye Nese Cinar |
| collection | DOAJ |
| description | Cyclospora cayetanensis is a human-specific coccidian parasite responsible for several food and water-related outbreaks around the world, including the most recent ones involving over 900 persons in 2013 and 2014 outbreaks in the USA. Multicopy organellar DNA such as mitochondrion genomes have been particularly informative for detection and genetic traceback analysis in other parasites. We sequenced the C. cayetanensis genomic DNA obtained from stool samples from patients infected with Cyclospora in Nepal using the Illumina MiSeq platform. By bioinformatically filtering out the metagenomic reads of non-coccidian origin sequences and concentrating the reads by targeted alignment, we were able to obtain contigs containing Eimeria-like mitochondrial, apicoplastic and some chromosomal genomic fragments. A mitochondrial genomic sequence was assembled and confirmed by cloning and sequencing targeted PCR products amplified from Cyclospora DNA using primers based on our draft assembly sequence. The results show that the C. cayetanensis mitochondrion genome is 6274 bp in length, with 33% GC content, and likely exists in concatemeric arrays as in Eimeria mitochondrial genomes. Phylogenetic analysis of the C. cayetanensis mitochondrial genome places this organism in a tight cluster with Eimeria species. The mitochondrial genome of C. cayetanensis contains three protein coding genes, cytochrome (cytb), cytochrome C oxidase subunit 1 (cox1), and cytochrome C oxidase subunit 3 (cox3), in addition to 14 large subunit (LSU) and nine small subunit (SSU) fragmented rRNA genes. |
| first_indexed | 2024-12-11T13:49:09Z |
| format | Article |
| id | doaj.art-58d32375772148d88b09e0532ed404cc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T13:49:09Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-58d32375772148d88b09e0532ed404cc2022-12-22T01:04:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e012864510.1371/journal.pone.0128645The Complete Mitochondrial Genome of the Foodborne Parasitic Pathogen Cyclospora cayetanensis.Hediye Nese CinarGopal GopinathKaren JarvisHelen R MurphyCyclospora cayetanensis is a human-specific coccidian parasite responsible for several food and water-related outbreaks around the world, including the most recent ones involving over 900 persons in 2013 and 2014 outbreaks in the USA. Multicopy organellar DNA such as mitochondrion genomes have been particularly informative for detection and genetic traceback analysis in other parasites. We sequenced the C. cayetanensis genomic DNA obtained from stool samples from patients infected with Cyclospora in Nepal using the Illumina MiSeq platform. By bioinformatically filtering out the metagenomic reads of non-coccidian origin sequences and concentrating the reads by targeted alignment, we were able to obtain contigs containing Eimeria-like mitochondrial, apicoplastic and some chromosomal genomic fragments. A mitochondrial genomic sequence was assembled and confirmed by cloning and sequencing targeted PCR products amplified from Cyclospora DNA using primers based on our draft assembly sequence. The results show that the C. cayetanensis mitochondrion genome is 6274 bp in length, with 33% GC content, and likely exists in concatemeric arrays as in Eimeria mitochondrial genomes. Phylogenetic analysis of the C. cayetanensis mitochondrial genome places this organism in a tight cluster with Eimeria species. The mitochondrial genome of C. cayetanensis contains three protein coding genes, cytochrome (cytb), cytochrome C oxidase subunit 1 (cox1), and cytochrome C oxidase subunit 3 (cox3), in addition to 14 large subunit (LSU) and nine small subunit (SSU) fragmented rRNA genes.http://europepmc.org/articles/PMC4455993?pdf=render |
| spellingShingle | Hediye Nese Cinar Gopal Gopinath Karen Jarvis Helen R Murphy The Complete Mitochondrial Genome of the Foodborne Parasitic Pathogen Cyclospora cayetanensis. PLoS ONE |
| title | The Complete Mitochondrial Genome of the Foodborne Parasitic Pathogen Cyclospora cayetanensis. |
| title_full | The Complete Mitochondrial Genome of the Foodborne Parasitic Pathogen Cyclospora cayetanensis. |
| title_fullStr | The Complete Mitochondrial Genome of the Foodborne Parasitic Pathogen Cyclospora cayetanensis. |
| title_full_unstemmed | The Complete Mitochondrial Genome of the Foodborne Parasitic Pathogen Cyclospora cayetanensis. |
| title_short | The Complete Mitochondrial Genome of the Foodborne Parasitic Pathogen Cyclospora cayetanensis. |
| title_sort | complete mitochondrial genome of the foodborne parasitic pathogen cyclospora cayetanensis |
| url | http://europepmc.org/articles/PMC4455993?pdf=render |
| work_keys_str_mv | AT hediyenesecinar thecompletemitochondrialgenomeofthefoodborneparasiticpathogencyclosporacayetanensis AT gopalgopinath thecompletemitochondrialgenomeofthefoodborneparasiticpathogencyclosporacayetanensis AT karenjarvis thecompletemitochondrialgenomeofthefoodborneparasiticpathogencyclosporacayetanensis AT helenrmurphy thecompletemitochondrialgenomeofthefoodborneparasiticpathogencyclosporacayetanensis AT hediyenesecinar completemitochondrialgenomeofthefoodborneparasiticpathogencyclosporacayetanensis AT gopalgopinath completemitochondrialgenomeofthefoodborneparasiticpathogencyclosporacayetanensis AT karenjarvis completemitochondrialgenomeofthefoodborneparasiticpathogencyclosporacayetanensis AT helenrmurphy completemitochondrialgenomeofthefoodborneparasiticpathogencyclosporacayetanensis |