Isolation and characterization of a human cementocyte-like cell line, HCY-23
Abstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, w...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
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| Series: | Brazilian Oral Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100255&lng=en&tlng=en |
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| author | Amanda Bandeira de ALMEIDA Elis Janaína Lira dos SANTOS Gabriel Flores ABUNA Cristiane Salmon RIBEIRO Márcio Zaffalon CASATI Karina Gonzales Silvério RUIZ Francisco Humberto NOCITI JUNIOR |
| author_facet | Amanda Bandeira de ALMEIDA Elis Janaína Lira dos SANTOS Gabriel Flores ABUNA Cristiane Salmon RIBEIRO Márcio Zaffalon CASATI Karina Gonzales Silvério RUIZ Francisco Humberto NOCITI JUNIOR |
| author_sort | Amanda Bandeira de ALMEIDA |
| collection | DOAJ |
| description | Abstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration. |
| first_indexed | 2024-12-13T11:52:51Z |
| format | Article |
| id | doaj.art-58d3286babb74326b19fc67f5bc19460 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-13T11:52:51Z |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-58d3286babb74326b19fc67f5bc194602022-12-21T23:47:18ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31073310.1590/1807-3107bor-2019.vol33.0058S1806-83242019000100255Isolation and characterization of a human cementocyte-like cell line, HCY-23Amanda Bandeira de ALMEIDAElis Janaína Lira dos SANTOSGabriel Flores ABUNACristiane Salmon RIBEIROMárcio Zaffalon CASATIKarina Gonzales Silvério RUIZFrancisco Humberto NOCITI JUNIORAbstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in cellular cementum, have the potential to be protagonist in cementum homeostasis, responding to endocrine signals and directing local cementum metabolism. Cells from healthy erupted human teeth were isolated using sequential collagenase/EDTA digestions, and maintained in standard cell culture conditions. A cementocyte-like cell line was cloned (HCY-23, for human cementocyte clone 23), which presented a cementocyte compatible gene expression signature, including the expression of dentin matrix protein 1 ( DMP1 ), sclerostin ( SOST ), and E11/gp38/podoplanin ( E11 ). In contrast, these cells did not express the odontoblast/dentin marker dentin sialoprotein ( DSPP ). HCY-23 cells produced mineral-like nodules in vitro under differentiation conditions, and were highly responsive to inorganic phosphate (Pi). Within the limits of the present study, it can be concluded that cementocytes are phosphate-responsive cells, and have the potential do play a key role in periodontal homeostasis and regeneration.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100255&lng=en&tlng=enDental CementumGene ExpressionCell Culture Techniques |
| spellingShingle | Amanda Bandeira de ALMEIDA Elis Janaína Lira dos SANTOS Gabriel Flores ABUNA Cristiane Salmon RIBEIRO Márcio Zaffalon CASATI Karina Gonzales Silvério RUIZ Francisco Humberto NOCITI JUNIOR Isolation and characterization of a human cementocyte-like cell line, HCY-23 Brazilian Oral Research Dental Cementum Gene Expression Cell Culture Techniques |
| title | Isolation and characterization of a human cementocyte-like cell line, HCY-23 |
| title_full | Isolation and characterization of a human cementocyte-like cell line, HCY-23 |
| title_fullStr | Isolation and characterization of a human cementocyte-like cell line, HCY-23 |
| title_full_unstemmed | Isolation and characterization of a human cementocyte-like cell line, HCY-23 |
| title_short | Isolation and characterization of a human cementocyte-like cell line, HCY-23 |
| title_sort | isolation and characterization of a human cementocyte like cell line hcy 23 |
| topic | Dental Cementum Gene Expression Cell Culture Techniques |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100255&lng=en&tlng=en |
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