Epidemiology and molecular characterization of the re-emerging measles virus among children and adults in the Haut-Ogooue, Gabon
Abstract Background Measles is one of the most infectious diseases with a high mortality rate worldwide. It is caused by the measles virus (MeV) which is a single stranded RNA virus with genetic diversity based on the nucleoprotein gene, including 24 genotypes. In Gabon, several outbreaks occurred i...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-01-01
|
| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12879-019-3731-y |
| _version_ | 1819207586418786304 |
|---|---|
| author | Sonia Etenna Lekana-Douki Pater Noster Sir-Ondo-Enguier Octavie Banga-Mve-Ella Romeo Karl Imboumy-Limoukou Gael D. Maganga Jean-Bernard Lekana-Douki Nicolas Berthet |
| author_facet | Sonia Etenna Lekana-Douki Pater Noster Sir-Ondo-Enguier Octavie Banga-Mve-Ella Romeo Karl Imboumy-Limoukou Gael D. Maganga Jean-Bernard Lekana-Douki Nicolas Berthet |
| author_sort | Sonia Etenna Lekana-Douki |
| collection | DOAJ |
| description | Abstract Background Measles is one of the most infectious diseases with a high mortality rate worldwide. It is caused by the measles virus (MeV) which is a single stranded RNA virus with genetic diversity based on the nucleoprotein gene, including 24 genotypes. In Gabon, several outbreaks occurred in the past few years, especially in 2016 in Libreville and Oyem. A surveillance network of infectious diseases highlighted a measles outbreak which occurred in the south of Gabon from April to June 2017. Methods Clinical specimens of urine, blood, throat and nasal swabs were collected in the two main cities of the Haut-Ogooue province, Franceville and Moanda. Virological investigations based on real-time polymerase chain reaction for molecular diagnosis and conventional PCR for genotype identification were done. Results Specimens were collected from 139 suspected measles patients. A total of 46 (33.1%) children and adults were laboratory-confirmed cases among which 16 (34.8%) were unvaccinated, 16 (34.8%) had received one dose, and 11 (23.9%) had received two doses of the measles vaccine. Phylogenetic analysis revealed that all the sequences of the nucleoprotein gene belonged to genotype B3. Conclusions Measles infection was more commonly confirmed among those with one recorded dose compared to suspect cases with none, unknown or two recorded doses. The molecular characterization of the strains showed the circulation of the B3 genotype which is endemic on the African continent, thirty years after the B2 genotype was described in an outbreak in Libreville, the capital of Gabon. These findings highlight that surveillance and molecular investigation of measles should be continued in Gabon. |
| first_indexed | 2024-12-23T05:25:51Z |
| format | Article |
| id | doaj.art-58d368d287494c00b9d83a327711f8b8 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-23T05:25:51Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-58d368d287494c00b9d83a327711f8b82022-12-21T17:58:36ZengBMCBMC Infectious Diseases1471-23342019-01-011911810.1186/s12879-019-3731-yEpidemiology and molecular characterization of the re-emerging measles virus among children and adults in the Haut-Ogooue, GabonSonia Etenna Lekana-Douki0Pater Noster Sir-Ondo-Enguier1Octavie Banga-Mve-Ella2Romeo Karl Imboumy-Limoukou3Gael D. Maganga4Jean-Bernard Lekana-Douki5Nicolas Berthet6Unité Emergence des maladies virales, Centre International de Recherches Médicales de FrancevilleUnité Evolution Epidémiologie et Résistances Parasitaires (UNEEREP), Centre International de Recherches Médicales de FrancevilleUnité Emergence des maladies virales, Centre International de Recherches Médicales de FrancevilleUnité Evolution Epidémiologie et Résistances Parasitaires (UNEEREP), Centre International de Recherches Médicales de FrancevilleUnité Emergence des maladies virales, Centre International de Recherches Médicales de FrancevilleUnité Evolution Epidémiologie et Résistances Parasitaires (UNEEREP), Centre International de Recherches Médicales de FrancevilleUnité Emergence des maladies virales, Centre International de Recherches Médicales de FrancevilleAbstract Background Measles is one of the most infectious diseases with a high mortality rate worldwide. It is caused by the measles virus (MeV) which is a single stranded RNA virus with genetic diversity based on the nucleoprotein gene, including 24 genotypes. In Gabon, several outbreaks occurred in the past few years, especially in 2016 in Libreville and Oyem. A surveillance network of infectious diseases highlighted a measles outbreak which occurred in the south of Gabon from April to June 2017. Methods Clinical specimens of urine, blood, throat and nasal swabs were collected in the two main cities of the Haut-Ogooue province, Franceville and Moanda. Virological investigations based on real-time polymerase chain reaction for molecular diagnosis and conventional PCR for genotype identification were done. Results Specimens were collected from 139 suspected measles patients. A total of 46 (33.1%) children and adults were laboratory-confirmed cases among which 16 (34.8%) were unvaccinated, 16 (34.8%) had received one dose, and 11 (23.9%) had received two doses of the measles vaccine. Phylogenetic analysis revealed that all the sequences of the nucleoprotein gene belonged to genotype B3. Conclusions Measles infection was more commonly confirmed among those with one recorded dose compared to suspect cases with none, unknown or two recorded doses. The molecular characterization of the strains showed the circulation of the B3 genotype which is endemic on the African continent, thirty years after the B2 genotype was described in an outbreak in Libreville, the capital of Gabon. These findings highlight that surveillance and molecular investigation of measles should be continued in Gabon.http://link.springer.com/article/10.1186/s12879-019-3731-yMeasles outbreakVaccinationStrainsHaut-OgooueGabon |
| spellingShingle | Sonia Etenna Lekana-Douki Pater Noster Sir-Ondo-Enguier Octavie Banga-Mve-Ella Romeo Karl Imboumy-Limoukou Gael D. Maganga Jean-Bernard Lekana-Douki Nicolas Berthet Epidemiology and molecular characterization of the re-emerging measles virus among children and adults in the Haut-Ogooue, Gabon BMC Infectious Diseases Measles outbreak Vaccination Strains Haut-Ogooue Gabon |
| title | Epidemiology and molecular characterization of the re-emerging measles virus among children and adults in the Haut-Ogooue, Gabon |
| title_full | Epidemiology and molecular characterization of the re-emerging measles virus among children and adults in the Haut-Ogooue, Gabon |
| title_fullStr | Epidemiology and molecular characterization of the re-emerging measles virus among children and adults in the Haut-Ogooue, Gabon |
| title_full_unstemmed | Epidemiology and molecular characterization of the re-emerging measles virus among children and adults in the Haut-Ogooue, Gabon |
| title_short | Epidemiology and molecular characterization of the re-emerging measles virus among children and adults in the Haut-Ogooue, Gabon |
| title_sort | epidemiology and molecular characterization of the re emerging measles virus among children and adults in the haut ogooue gabon |
| topic | Measles outbreak Vaccination Strains Haut-Ogooue Gabon |
| url | http://link.springer.com/article/10.1186/s12879-019-3731-y |
| work_keys_str_mv | AT soniaetennalekanadouki epidemiologyandmolecularcharacterizationofthereemergingmeaslesvirusamongchildrenandadultsinthehautogoouegabon AT paternostersirondoenguier epidemiologyandmolecularcharacterizationofthereemergingmeaslesvirusamongchildrenandadultsinthehautogoouegabon AT octaviebangamveella epidemiologyandmolecularcharacterizationofthereemergingmeaslesvirusamongchildrenandadultsinthehautogoouegabon AT romeokarlimboumylimoukou epidemiologyandmolecularcharacterizationofthereemergingmeaslesvirusamongchildrenandadultsinthehautogoouegabon AT gaeldmaganga epidemiologyandmolecularcharacterizationofthereemergingmeaslesvirusamongchildrenandadultsinthehautogoouegabon AT jeanbernardlekanadouki epidemiologyandmolecularcharacterizationofthereemergingmeaslesvirusamongchildrenandadultsinthehautogoouegabon AT nicolasberthet epidemiologyandmolecularcharacterizationofthereemergingmeaslesvirusamongchildrenandadultsinthehautogoouegabon |