<i>Arthrobotrys musiformis</i> (Orbiliales) Kills <i>Haemonchus contortus</i> Infective Larvae (Trichostronylidae) through Its Predatory Activity and Its Fungal Culture Filtrates
<i>Haemonchus contortus</i> (Hc) is a parasite affecting small ruminants worldwide. <i>Arthrobotrys musiformis</i> (Am) is a nematode-trapping fungi that captures, destroys and feeds on nematodes. This study assessed the predatory activity (PA) and nematocidal activity (NA) o...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/10/1068 |
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| author | Gustavo Pérez-Anzúrez Agustín Olmedo-Juárez Elke von-Son de Fernex Miguel Ángel Alonso-Díaz Edgar Jesús Delgado-Núñez María Eugenia López-Arellano Manasés González-Cortázar Alejandro Zamilpa Ana Yuridia Ocampo-Gutierrez Adolfo Paz-Silva Pedro Mendoza-de Gives |
| author_facet | Gustavo Pérez-Anzúrez Agustín Olmedo-Juárez Elke von-Son de Fernex Miguel Ángel Alonso-Díaz Edgar Jesús Delgado-Núñez María Eugenia López-Arellano Manasés González-Cortázar Alejandro Zamilpa Ana Yuridia Ocampo-Gutierrez Adolfo Paz-Silva Pedro Mendoza-de Gives |
| author_sort | Gustavo Pérez-Anzúrez |
| collection | DOAJ |
| description | <i>Haemonchus contortus</i> (Hc) is a parasite affecting small ruminants worldwide. <i>Arthrobotrys musiformis</i> (Am) is a nematode-trapping fungi that captures, destroys and feeds on nematodes. This study assessed the predatory activity (PA) and nematocidal activity (NA) of liquid culture filtrates (LCF) of Am against Hc infective larvae (L3), and additionally, the mycochemical profile (MP) was performed. Fungal identification was achieved by traditional and molecular procedures. The PA of Am against HcL3 was performed in water agar plates. Means of non-predated larvae were recorded and compared with a control group without fungi. LCF/HcL3 interaction was performed using micro-tittering plates. Two media, Czapek–Dox broth (CDB) and sweet potato dextrose broth (SPDB) and three concentrations, were assessed. Lectures were performed after 48 h interaction. The means of alive and dead larvae were recorded and compared with proper negative controls. The PA assessment revealed 71.54% larval reduction (<i>p</i> < 0.01). The highest NA of LCF was found in CDB: 93.42, 73.02 and 51.61%, at 100, 50 and 25 mg/mL, respectively (<i>p</i> < 0.05). Alkaloids and saponins were identified in both media; meanwhile, coumarins were only identified in CDB. The NA was only found in CDB, but not in SPDB. Coumarins could be responsible for the NA. |
| first_indexed | 2024-03-09T19:38:13Z |
| format | Article |
| id | doaj.art-58d3f4bc6f20481db98779c201b12f2e |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T19:38:13Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-58d3f4bc6f20481db98779c201b12f2e2023-11-24T01:47:00ZengMDPI AGPathogens2076-08172022-09-011110106810.3390/pathogens11101068<i>Arthrobotrys musiformis</i> (Orbiliales) Kills <i>Haemonchus contortus</i> Infective Larvae (Trichostronylidae) through Its Predatory Activity and Its Fungal Culture FiltratesGustavo Pérez-Anzúrez0Agustín Olmedo-Juárez1Elke von-Son de Fernex2Miguel Ángel Alonso-Díaz3Edgar Jesús Delgado-Núñez4María Eugenia López-Arellano5Manasés González-Cortázar6Alejandro Zamilpa7Ana Yuridia Ocampo-Gutierrez8Adolfo Paz-Silva9Pedro Mendoza-de Gives10Laboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Morelos, Jiutepec CP 62550, MexicoLaboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Morelos, Jiutepec CP 62550, MexicoTropical Livestock Center, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Martínez de la Torre CP 93600, MexicoTropical Livestock Center, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Martínez de la Torre CP 93600, MexicoFaculty of Agricultural, Livestock and Environmental Sciences, Autonomous University of the State of Guerrero, Iguala de la Independencia CP 40040, MexicoLaboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Morelos, Jiutepec CP 62550, MexicoSouth Biomedical Research Center, Social Security Mexican Institute (CIBIS-IMSS), Xochitepec CP 62790, MexicoSouth Biomedical Research Center, Social Security Mexican Institute (CIBIS-IMSS), Xochitepec CP 62790, MexicoLaboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Morelos, Jiutepec CP 62550, MexicoDepartment of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27142 Lugo, SpainLaboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Morelos, Jiutepec CP 62550, Mexico<i>Haemonchus contortus</i> (Hc) is a parasite affecting small ruminants worldwide. <i>Arthrobotrys musiformis</i> (Am) is a nematode-trapping fungi that captures, destroys and feeds on nematodes. This study assessed the predatory activity (PA) and nematocidal activity (NA) of liquid culture filtrates (LCF) of Am against Hc infective larvae (L3), and additionally, the mycochemical profile (MP) was performed. Fungal identification was achieved by traditional and molecular procedures. The PA of Am against HcL3 was performed in water agar plates. Means of non-predated larvae were recorded and compared with a control group without fungi. LCF/HcL3 interaction was performed using micro-tittering plates. Two media, Czapek–Dox broth (CDB) and sweet potato dextrose broth (SPDB) and three concentrations, were assessed. Lectures were performed after 48 h interaction. The means of alive and dead larvae were recorded and compared with proper negative controls. The PA assessment revealed 71.54% larval reduction (<i>p</i> < 0.01). The highest NA of LCF was found in CDB: 93.42, 73.02 and 51.61%, at 100, 50 and 25 mg/mL, respectively (<i>p</i> < 0.05). Alkaloids and saponins were identified in both media; meanwhile, coumarins were only identified in CDB. The NA was only found in CDB, but not in SPDB. Coumarins could be responsible for the NA.https://www.mdpi.com/2076-0817/11/10/1068nematophagous fungi<i>Arthrobotrys</i>nematodes predationbiocontrolnatural compounds |
| spellingShingle | Gustavo Pérez-Anzúrez Agustín Olmedo-Juárez Elke von-Son de Fernex Miguel Ángel Alonso-Díaz Edgar Jesús Delgado-Núñez María Eugenia López-Arellano Manasés González-Cortázar Alejandro Zamilpa Ana Yuridia Ocampo-Gutierrez Adolfo Paz-Silva Pedro Mendoza-de Gives <i>Arthrobotrys musiformis</i> (Orbiliales) Kills <i>Haemonchus contortus</i> Infective Larvae (Trichostronylidae) through Its Predatory Activity and Its Fungal Culture Filtrates Pathogens nematophagous fungi <i>Arthrobotrys</i> nematodes predation biocontrol natural compounds |
| title | <i>Arthrobotrys musiformis</i> (Orbiliales) Kills <i>Haemonchus contortus</i> Infective Larvae (Trichostronylidae) through Its Predatory Activity and Its Fungal Culture Filtrates |
| title_full | <i>Arthrobotrys musiformis</i> (Orbiliales) Kills <i>Haemonchus contortus</i> Infective Larvae (Trichostronylidae) through Its Predatory Activity and Its Fungal Culture Filtrates |
| title_fullStr | <i>Arthrobotrys musiformis</i> (Orbiliales) Kills <i>Haemonchus contortus</i> Infective Larvae (Trichostronylidae) through Its Predatory Activity and Its Fungal Culture Filtrates |
| title_full_unstemmed | <i>Arthrobotrys musiformis</i> (Orbiliales) Kills <i>Haemonchus contortus</i> Infective Larvae (Trichostronylidae) through Its Predatory Activity and Its Fungal Culture Filtrates |
| title_short | <i>Arthrobotrys musiformis</i> (Orbiliales) Kills <i>Haemonchus contortus</i> Infective Larvae (Trichostronylidae) through Its Predatory Activity and Its Fungal Culture Filtrates |
| title_sort | i arthrobotrys musiformis i orbiliales kills i haemonchus contortus i infective larvae trichostronylidae through its predatory activity and its fungal culture filtrates |
| topic | nematophagous fungi <i>Arthrobotrys</i> nematodes predation biocontrol natural compounds |
| url | https://www.mdpi.com/2076-0817/11/10/1068 |
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