Performance of Wayne assay for detection of pyrazinamide resistance in Mycobacterium tuberculosis: a meta-analysis study
Conventional culture-based drug susceptibility testing (DST) of Mycobacterium tuberculosis to pyrazinamide (PZA) is time-consuming and difficult to perform. The current systematic review and meta-analysis was aimed to evaluate the diagnostic accuracy of Wayne assay against culture-based DSTs as the...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | New Microbes and New Infections |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2052297521000500 |
| _version_ | 1819124253134422016 |
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| author | M.J. Nasiri F. Fardsanei M. Arshadi B. Deihim Farima Khalili M. Dadashi M. Goudarzi M. Mirsaeidi |
| author_facet | M.J. Nasiri F. Fardsanei M. Arshadi B. Deihim Farima Khalili M. Dadashi M. Goudarzi M. Mirsaeidi |
| author_sort | M.J. Nasiri |
| collection | DOAJ |
| description | Conventional culture-based drug susceptibility testing (DST) of Mycobacterium tuberculosis to pyrazinamide (PZA) is time-consuming and difficult to perform. The current systematic review and meta-analysis was aimed to evaluate the diagnostic accuracy of Wayne assay against culture-based DSTs as the reference standard. We searched the MEDLINE/Pubmed, Embase, and Web of Science databases for the relevant records. The QUADAS-2 tool was used to assess the quality of the studies. Diagnostic accuracy measures (i.e., sensitivity and specificity) were pooled with a random-effects model. Statistical analyses were performed with STATA (version 14, Stata Corporation, College Station, TX, USA), RevMan (version 5.3; The Nordic Cochrane Centre, the Cochrane Collaboration, Copenhagen, Denmark), and Meta-DiSc (version 1.4, Cochrane Colloquium, Barcelona, Spain). A total of 31 articles comprising data for 2457 isolates of M. tuberculosis were included in the final analysis. The pooled sensitivity and specificity of the Wayne assay against all reference tests (the combination of BACTEC MGIT 960, BACTEC 460, and proportion method) were 86.6% (95% CI: 84.3-88.7) and 96.0% (95% CI: 94.8-97). The positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and the area under the curve (AUC) estimates were found to be 17.6 (95% CI: 10.5-29.3), 0.11 (95% CI: 0.06-0.20), 164 (95% CI: 83-320) and 97%, respectively. Deek's test result indicated no evidence for publication bias (P > 0.05).Although the current study shows that the Wayne test is sensitive and specific for detecting PZA resistance, it may be used in combination with conventional DSTs to diagnose PZA resistance accurately. |
| first_indexed | 2024-12-22T07:21:18Z |
| format | Article |
| id | doaj.art-58d6d47ec01c456db63b6b194338d1bc |
| institution | Directory Open Access Journal |
| issn | 2052-2975 |
| language | English |
| last_indexed | 2024-12-22T07:21:18Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | New Microbes and New Infections |
| spelling | doaj.art-58d6d47ec01c456db63b6b194338d1bc2022-12-21T18:34:16ZengElsevierNew Microbes and New Infections2052-29752021-07-0142100886Performance of Wayne assay for detection of pyrazinamide resistance in Mycobacterium tuberculosis: a meta-analysis studyM.J. Nasiri0F. Fardsanei1M. Arshadi2B. Deihim3Farima Khalili4M. Dadashi5M. Goudarzi6M. Mirsaeidi7Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Corresponding author: M. J. Nasiri, Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranInfectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Bacteriology and Virology, School of Medicine, Dezful University of Medical Sciences, Dezful, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Pulmonary and Critical Care, University of Miami Miller School of Medicine, Miami, FL, USA; Corresponding author: M. Mirsaeidi, Department of Pulmonary and Critical Care, University of Miami Miller School of Medicine, Miami, FL, USA.Conventional culture-based drug susceptibility testing (DST) of Mycobacterium tuberculosis to pyrazinamide (PZA) is time-consuming and difficult to perform. The current systematic review and meta-analysis was aimed to evaluate the diagnostic accuracy of Wayne assay against culture-based DSTs as the reference standard. We searched the MEDLINE/Pubmed, Embase, and Web of Science databases for the relevant records. The QUADAS-2 tool was used to assess the quality of the studies. Diagnostic accuracy measures (i.e., sensitivity and specificity) were pooled with a random-effects model. Statistical analyses were performed with STATA (version 14, Stata Corporation, College Station, TX, USA), RevMan (version 5.3; The Nordic Cochrane Centre, the Cochrane Collaboration, Copenhagen, Denmark), and Meta-DiSc (version 1.4, Cochrane Colloquium, Barcelona, Spain). A total of 31 articles comprising data for 2457 isolates of M. tuberculosis were included in the final analysis. The pooled sensitivity and specificity of the Wayne assay against all reference tests (the combination of BACTEC MGIT 960, BACTEC 460, and proportion method) were 86.6% (95% CI: 84.3-88.7) and 96.0% (95% CI: 94.8-97). The positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and the area under the curve (AUC) estimates were found to be 17.6 (95% CI: 10.5-29.3), 0.11 (95% CI: 0.06-0.20), 164 (95% CI: 83-320) and 97%, respectively. Deek's test result indicated no evidence for publication bias (P > 0.05).Although the current study shows that the Wayne test is sensitive and specific for detecting PZA resistance, it may be used in combination with conventional DSTs to diagnose PZA resistance accurately.http://www.sciencedirect.com/science/article/pii/S2052297521000500Pyrazinamideresistancesystematic reviewtuberculosisWayne assay |
| spellingShingle | M.J. Nasiri F. Fardsanei M. Arshadi B. Deihim Farima Khalili M. Dadashi M. Goudarzi M. Mirsaeidi Performance of Wayne assay for detection of pyrazinamide resistance in Mycobacterium tuberculosis: a meta-analysis study New Microbes and New Infections Pyrazinamide resistance systematic review tuberculosis Wayne assay |
| title | Performance of Wayne assay for detection of pyrazinamide resistance in Mycobacterium tuberculosis: a meta-analysis study |
| title_full | Performance of Wayne assay for detection of pyrazinamide resistance in Mycobacterium tuberculosis: a meta-analysis study |
| title_fullStr | Performance of Wayne assay for detection of pyrazinamide resistance in Mycobacterium tuberculosis: a meta-analysis study |
| title_full_unstemmed | Performance of Wayne assay for detection of pyrazinamide resistance in Mycobacterium tuberculosis: a meta-analysis study |
| title_short | Performance of Wayne assay for detection of pyrazinamide resistance in Mycobacterium tuberculosis: a meta-analysis study |
| title_sort | performance of wayne assay for detection of pyrazinamide resistance in mycobacterium tuberculosis a meta analysis study |
| topic | Pyrazinamide resistance systematic review tuberculosis Wayne assay |
| url | http://www.sciencedirect.com/science/article/pii/S2052297521000500 |
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