Storage Effects on Bioactive Phenols in Calabrian Monovarietal Extra Virgin Olive Oils Based on the EFSA Health Claim
The beneficial properties of extra virgin olive oil (EVOO) on lipids blood levels were recognized by the European Food Safety Authority (EFSA) with a health claim, specifically referring to EVOOs containing at least 5 mg of hydroxytyrosol and its secoiridoids derivatives per 20 g of oil. The main pu...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/12/20/3799 |
| _version_ | 1797573833762275328 |
|---|---|
| author | Marialaura Frisina Sonia Bonacci Manuela Oliverio Monica Nardi Thomas Patrizio Vatrano Antonio Procopio |
| author_facet | Marialaura Frisina Sonia Bonacci Manuela Oliverio Monica Nardi Thomas Patrizio Vatrano Antonio Procopio |
| author_sort | Marialaura Frisina |
| collection | DOAJ |
| description | The beneficial properties of extra virgin olive oil (EVOO) on lipids blood levels were recognized by the European Food Safety Authority (EFSA) with a health claim, specifically referring to EVOOs containing at least 5 mg of hydroxytyrosol and its secoiridoids derivatives per 20 g of oil. The main purpose of the work was to characterize the phenolic profile of two commercially available Calabrian monovarietal EVOOs (<i>Nocellara del Belice</i>, VN; <i>Dolce di Rossano</i>, VDR), and to study the effect of one-year storage on secoiridoids composition, by monthly controls. A new UHPLC-ESI-HRMS method was developed and validated, thus facilitating the EFSA claim application and allowing producers to valorize their products. Seven biologically active compounds were chosen: tyrosol, hydroxytyrosol, oleocanthal, oleacein, oleuropein aglycone, verbascoside, and oleuropein. LODs and LOQs were 0.001–0.02 mg g<sup>−1</sup> and 0.002–0.08 mg g<sup>−1</sup>, respectively. The variation coefficients were ≤20% and the percentage of recovery was between 89–109%. During the 12-month storage period, the concentration of selected compounds ranged between 1258.78–1478.91 mg Kg<sup>−1</sup> for VN, and 1408.22–2071.45 mg Kg<sup>−1</sup> for VDR, with a decrease of 15% and 32% respectively. The method allows an accurate quantification of EVOO phenols thus being useful to certify the nutraceutical properties of olive oil. |
| first_indexed | 2024-03-10T21:14:37Z |
| format | Article |
| id | doaj.art-58d88262da6e42599aeb4742edbeebba |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T21:14:37Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-58d88262da6e42599aeb4742edbeebba2023-11-19T16:29:58ZengMDPI AGFoods2304-81582023-10-011220379910.3390/foods12203799Storage Effects on Bioactive Phenols in Calabrian Monovarietal Extra Virgin Olive Oils Based on the EFSA Health ClaimMarialaura Frisina0Sonia Bonacci1Manuela Oliverio2Monica Nardi3Thomas Patrizio Vatrano4Antonio Procopio5Department of Health Science, University Magna Græcia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Science, University Magna Græcia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Science, University Magna Græcia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Science, University Magna Græcia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Science, University Magna Græcia of Catanzaro, 88100 Catanzaro, ItalyDepartment of Health Science, University Magna Græcia of Catanzaro, 88100 Catanzaro, ItalyThe beneficial properties of extra virgin olive oil (EVOO) on lipids blood levels were recognized by the European Food Safety Authority (EFSA) with a health claim, specifically referring to EVOOs containing at least 5 mg of hydroxytyrosol and its secoiridoids derivatives per 20 g of oil. The main purpose of the work was to characterize the phenolic profile of two commercially available Calabrian monovarietal EVOOs (<i>Nocellara del Belice</i>, VN; <i>Dolce di Rossano</i>, VDR), and to study the effect of one-year storage on secoiridoids composition, by monthly controls. A new UHPLC-ESI-HRMS method was developed and validated, thus facilitating the EFSA claim application and allowing producers to valorize their products. Seven biologically active compounds were chosen: tyrosol, hydroxytyrosol, oleocanthal, oleacein, oleuropein aglycone, verbascoside, and oleuropein. LODs and LOQs were 0.001–0.02 mg g<sup>−1</sup> and 0.002–0.08 mg g<sup>−1</sup>, respectively. The variation coefficients were ≤20% and the percentage of recovery was between 89–109%. During the 12-month storage period, the concentration of selected compounds ranged between 1258.78–1478.91 mg Kg<sup>−1</sup> for VN, and 1408.22–2071.45 mg Kg<sup>−1</sup> for VDR, with a decrease of 15% and 32% respectively. The method allows an accurate quantification of EVOO phenols thus being useful to certify the nutraceutical properties of olive oil.https://www.mdpi.com/2304-8158/12/20/3799secoiridoidsextra virgin olive oil (EVOO)high-resolution mass spectrometrymethod validation |
| spellingShingle | Marialaura Frisina Sonia Bonacci Manuela Oliverio Monica Nardi Thomas Patrizio Vatrano Antonio Procopio Storage Effects on Bioactive Phenols in Calabrian Monovarietal Extra Virgin Olive Oils Based on the EFSA Health Claim Foods secoiridoids extra virgin olive oil (EVOO) high-resolution mass spectrometry method validation |
| title | Storage Effects on Bioactive Phenols in Calabrian Monovarietal Extra Virgin Olive Oils Based on the EFSA Health Claim |
| title_full | Storage Effects on Bioactive Phenols in Calabrian Monovarietal Extra Virgin Olive Oils Based on the EFSA Health Claim |
| title_fullStr | Storage Effects on Bioactive Phenols in Calabrian Monovarietal Extra Virgin Olive Oils Based on the EFSA Health Claim |
| title_full_unstemmed | Storage Effects on Bioactive Phenols in Calabrian Monovarietal Extra Virgin Olive Oils Based on the EFSA Health Claim |
| title_short | Storage Effects on Bioactive Phenols in Calabrian Monovarietal Extra Virgin Olive Oils Based on the EFSA Health Claim |
| title_sort | storage effects on bioactive phenols in calabrian monovarietal extra virgin olive oils based on the efsa health claim |
| topic | secoiridoids extra virgin olive oil (EVOO) high-resolution mass spectrometry method validation |
| url | https://www.mdpi.com/2304-8158/12/20/3799 |
| work_keys_str_mv | AT marialaurafrisina storageeffectsonbioactivephenolsincalabrianmonovarietalextravirginoliveoilsbasedontheefsahealthclaim AT soniabonacci storageeffectsonbioactivephenolsincalabrianmonovarietalextravirginoliveoilsbasedontheefsahealthclaim AT manuelaoliverio storageeffectsonbioactivephenolsincalabrianmonovarietalextravirginoliveoilsbasedontheefsahealthclaim AT monicanardi storageeffectsonbioactivephenolsincalabrianmonovarietalextravirginoliveoilsbasedontheefsahealthclaim AT thomaspatriziovatrano storageeffectsonbioactivephenolsincalabrianmonovarietalextravirginoliveoilsbasedontheefsahealthclaim AT antonioprocopio storageeffectsonbioactivephenolsincalabrianmonovarietalextravirginoliveoilsbasedontheefsahealthclaim |