Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan Province
Background: Selenoprotein P (Sepp1) is the major selenoprotein in plasma and contains over 50% of the total plasma. The direct and indirect effects of Sepp1 on anti-oxidant defense can be responsible for its role in cancer risk. One of the common polymorphism of SEPP1gene is the Ala234Thr single nuc...
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| Format: | Article |
| Language: | English |
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Bushehr University of Medical Sciences
2018-01-01
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| Series: | Iranian South Medical Journal |
| Subjects: | |
| Online Access: | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-1-21&slc_lang=en&sid=1 |
| _version_ | 1811262595146973184 |
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| author | Samaneh Mohammaddoust Zivar Salehi Hamid Saeidi Saedi |
| author_facet | Samaneh Mohammaddoust Zivar Salehi Hamid Saeidi Saedi |
| author_sort | Samaneh Mohammaddoust |
| collection | DOAJ |
| description | Background: Selenoprotein P (Sepp1) is the major selenoprotein in plasma and contains over 50% of the total plasma. The direct and indirect effects of Sepp1 on anti-oxidant defense can be responsible for its role in cancer risk. One of the common polymorphism of SEPP1gene is the Ala234Thr single nucleotide polymorphism (SNP) in coding region. In this research we studied the association between SEPP1 Ala234Thr (rs3877899) polymorphism with the risk of breast cancer.
Materials and Methods: In this case-control study, 162 patients with breast cancer were compared with 227 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes. Determination of DNA genotyping in SEPP1 Ala234Thr (rs3877899) polymorphism was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) by using MwoI enzyme. Statistical analysis was performed by using the MedCalc program and χ2-test.
Results: The genotype frequencies were significantly different between the cases and controls. AA homozygosity subjects were more at risk for breast cancer than others (OR=3.23; 95%CI, 1.79-5.84; p=0.001).
Conclusion:Based on the results of this study, The Ala234Thr polymorphism of the SEPP1 gene may be associated with the potential for breast cancer in the female population of northern Iranian women. However, studiesneed to be conducted in larger populations to confirm these results. |
| first_indexed | 2024-04-12T19:28:18Z |
| format | Article |
| id | doaj.art-58dab61088d34ae199f59d399c73f5d6 |
| institution | Directory Open Access Journal |
| issn | 1735-4374 1735-6954 |
| language | English |
| last_indexed | 2024-04-12T19:28:18Z |
| publishDate | 2018-01-01 |
| publisher | Bushehr University of Medical Sciences |
| record_format | Article |
| series | Iranian South Medical Journal |
| spelling | doaj.art-58dab61088d34ae199f59d399c73f5d62022-12-22T03:19:24ZengBushehr University of Medical SciencesIranian South Medical Journal1735-43741735-69542018-01-01206519526Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan ProvinceSamaneh Mohammaddoust0Zivar Salehi1Hamid Saeidi Saedi2 Department of Biology, School of Sciences, University of Guilan, Rasht, Iran Department of Biology, School of Sciences, University of Guilan, Rasht, Iran Department of Oncology, Cancer Research Center, Guilan University of Medical Sciences, Rasht, Iran Background: Selenoprotein P (Sepp1) is the major selenoprotein in plasma and contains over 50% of the total plasma. The direct and indirect effects of Sepp1 on anti-oxidant defense can be responsible for its role in cancer risk. One of the common polymorphism of SEPP1gene is the Ala234Thr single nucleotide polymorphism (SNP) in coding region. In this research we studied the association between SEPP1 Ala234Thr (rs3877899) polymorphism with the risk of breast cancer. Materials and Methods: In this case-control study, 162 patients with breast cancer were compared with 227 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes. Determination of DNA genotyping in SEPP1 Ala234Thr (rs3877899) polymorphism was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) by using MwoI enzyme. Statistical analysis was performed by using the MedCalc program and χ2-test. Results: The genotype frequencies were significantly different between the cases and controls. AA homozygosity subjects were more at risk for breast cancer than others (OR=3.23; 95%CI, 1.79-5.84; p=0.001). Conclusion:Based on the results of this study, The Ala234Thr polymorphism of the SEPP1 gene may be associated with the potential for breast cancer in the female population of northern Iranian women. However, studiesneed to be conducted in larger populations to confirm these results.http://ismj.bpums.ac.ir/browse.php?a_code=A-10-1-21&slc_lang=en&sid=1Guilan province polymorphism Breast cancer Selenoprotein Ala234Thr SEPP1 |
| spellingShingle | Samaneh Mohammaddoust Zivar Salehi Hamid Saeidi Saedi Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan Province Iranian South Medical Journal Guilan province polymorphism Breast cancer Selenoprotein Ala234Thr SEPP1 |
| title | Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan Province |
| title_full | Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan Province |
| title_fullStr | Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan Province |
| title_full_unstemmed | Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan Province |
| title_short | Analysis of Ala234Thr Polymorphism SEPP1 gene in Women with Breast Cancer in Guilan Province |
| title_sort | analysis of ala234thr polymorphism sepp1 gene in women with breast cancer in guilan province |
| topic | Guilan province polymorphism Breast cancer Selenoprotein Ala234Thr SEPP1 |
| url | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-1-21&slc_lang=en&sid=1 |
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