Antibiotic Resistance and Virulence of Extraintestinal Pathogenic Escherichia coli (ExPEC) Vary According to Molecular Types
Extraintestinal pathogenic Escherichia coli (ExPEC) can cause many human extraintestinal infections. Resistance and virulence of ExPEC are inextricably linked to its phylogenetic background. However, studies on type-specific distribution of resistance and virulence and the connection between resista...
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| Language: | English |
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.598305/full |
| _version_ | 1818880251266072576 |
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| author | Yitao Duan Huihui Gao Liyang Zheng Shuangqing Liu Yang Cao Siyuan Zhu Zhenzhe Wu Hongqiang Ren Daqing Mao Yi Luo Yi Luo |
| author_facet | Yitao Duan Huihui Gao Liyang Zheng Shuangqing Liu Yang Cao Siyuan Zhu Zhenzhe Wu Hongqiang Ren Daqing Mao Yi Luo Yi Luo |
| author_sort | Yitao Duan |
| collection | DOAJ |
| description | Extraintestinal pathogenic Escherichia coli (ExPEC) can cause many human extraintestinal infections. Resistance and virulence of ExPEC are inextricably linked to its phylogenetic background. However, studies on type-specific distribution of resistance and virulence and the connection between resistance/virulence and molecular typing are lacking. Here, 411 ExPEC strains were collected and characterized using antimicrobial susceptibility testing and molecular typing. Among these, 74 representative strains were selected for whole genome sequencing and the Galleria mellonella killing assay. CH40-30-ST131, CH37-27-ST405, CH40-41-ST131, and CH13-5-ST12 isolates had high resistance rates to all antimicrobials tested. BlaCTX–M played a significant role in the β-lactam resistance of ExPEC isolates. CH14-64-ST1193, CH40-30-ST131, and CH35-27-ST69 isolates were highly virulent in the G. mellonella model. Virulence factors (VFs) involved in adherence (papB, papI, papX, and fimA), autotransporter (sat), invasion (aslA, kpsD), iron uptake (except for entD), or toxin (senB) might be responsible for pathogenicity in vivo. Specific antibiotic resistance genes (ARGs) or VFs were prevalent in specific types of strains, including papB, papI, fimA, sat, kpsD, senB, and aerobactin genes in CH14-64-ST1193 isolates; blaCTX–M–15, aac(6′)-Ib-cr, papB, papI, sat, iucA, iucB, iucC, chuT, chuX, and shuU in CH40-30-ST131 isolates; tetB in CH35-27-ST69 and CH13-5-ST12 isolates. Type distribution also differed by VF score. CH37-27-ST405 and CH26-5-ST38 isolates carried more ARGs and VFs indicating that they had a high resistance and virulence potential. This study demonstrates the type-specific distribution of resistance and virulence thus providing a basis for further research, prevention and treatment of ExPEC infections. |
| first_indexed | 2024-12-19T14:43:00Z |
| format | Article |
| id | doaj.art-58e19f87facf45efabcb8a8d7d6f636b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T14:43:00Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-58e19f87facf45efabcb8a8d7d6f636b2022-12-21T20:17:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-11-011110.3389/fmicb.2020.598305598305Antibiotic Resistance and Virulence of Extraintestinal Pathogenic Escherichia coli (ExPEC) Vary According to Molecular TypesYitao Duan0Huihui Gao1Liyang Zheng2Shuangqing Liu3Yang Cao4Siyuan Zhu5Zhenzhe Wu6Hongqiang Ren7Daqing Mao8Yi Luo9Yi Luo10College of Environmental Science and Engineering, Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Nankai University, Tianjin, ChinaSchool of Medicine, Nankai University, Tianjin, ChinaSchool of Medicine, Nankai University, Tianjin, ChinaDepartment of Clinical Laboratory, The Second Hospital of Tianjin Medical University, Tianjin, ChinaDepartment of Clinical Laboratory, The Second Hospital of Tianjin Medical University, Tianjin, ChinaCollege of Environmental Science and Engineering, Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Nankai University, Tianjin, ChinaCollege of Environmental Science and Engineering, Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Nankai University, Tianjin, ChinaState Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, ChinaSchool of Medicine, Nankai University, Tianjin, ChinaCollege of Environmental Science and Engineering, Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Nankai University, Tianjin, ChinaState Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, ChinaExtraintestinal pathogenic Escherichia coli (ExPEC) can cause many human extraintestinal infections. Resistance and virulence of ExPEC are inextricably linked to its phylogenetic background. However, studies on type-specific distribution of resistance and virulence and the connection between resistance/virulence and molecular typing are lacking. Here, 411 ExPEC strains were collected and characterized using antimicrobial susceptibility testing and molecular typing. Among these, 74 representative strains were selected for whole genome sequencing and the Galleria mellonella killing assay. CH40-30-ST131, CH37-27-ST405, CH40-41-ST131, and CH13-5-ST12 isolates had high resistance rates to all antimicrobials tested. BlaCTX–M played a significant role in the β-lactam resistance of ExPEC isolates. CH14-64-ST1193, CH40-30-ST131, and CH35-27-ST69 isolates were highly virulent in the G. mellonella model. Virulence factors (VFs) involved in adherence (papB, papI, papX, and fimA), autotransporter (sat), invasion (aslA, kpsD), iron uptake (except for entD), or toxin (senB) might be responsible for pathogenicity in vivo. Specific antibiotic resistance genes (ARGs) or VFs were prevalent in specific types of strains, including papB, papI, fimA, sat, kpsD, senB, and aerobactin genes in CH14-64-ST1193 isolates; blaCTX–M–15, aac(6′)-Ib-cr, papB, papI, sat, iucA, iucB, iucC, chuT, chuX, and shuU in CH40-30-ST131 isolates; tetB in CH35-27-ST69 and CH13-5-ST12 isolates. Type distribution also differed by VF score. CH37-27-ST405 and CH26-5-ST38 isolates carried more ARGs and VFs indicating that they had a high resistance and virulence potential. This study demonstrates the type-specific distribution of resistance and virulence thus providing a basis for further research, prevention and treatment of ExPEC infections.https://www.frontiersin.org/articles/10.3389/fmicb.2020.598305/fullextraintestinal pathogenic Escherichia coliCH typingmultilocus sequence typingresistancevirulence |
| spellingShingle | Yitao Duan Huihui Gao Liyang Zheng Shuangqing Liu Yang Cao Siyuan Zhu Zhenzhe Wu Hongqiang Ren Daqing Mao Yi Luo Yi Luo Antibiotic Resistance and Virulence of Extraintestinal Pathogenic Escherichia coli (ExPEC) Vary According to Molecular Types Frontiers in Microbiology extraintestinal pathogenic Escherichia coli CH typing multilocus sequence typing resistance virulence |
| title | Antibiotic Resistance and Virulence of Extraintestinal Pathogenic Escherichia coli (ExPEC) Vary According to Molecular Types |
| title_full | Antibiotic Resistance and Virulence of Extraintestinal Pathogenic Escherichia coli (ExPEC) Vary According to Molecular Types |
| title_fullStr | Antibiotic Resistance and Virulence of Extraintestinal Pathogenic Escherichia coli (ExPEC) Vary According to Molecular Types |
| title_full_unstemmed | Antibiotic Resistance and Virulence of Extraintestinal Pathogenic Escherichia coli (ExPEC) Vary According to Molecular Types |
| title_short | Antibiotic Resistance and Virulence of Extraintestinal Pathogenic Escherichia coli (ExPEC) Vary According to Molecular Types |
| title_sort | antibiotic resistance and virulence of extraintestinal pathogenic escherichia coli expec vary according to molecular types |
| topic | extraintestinal pathogenic Escherichia coli CH typing multilocus sequence typing resistance virulence |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.598305/full |
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