Protective Effect of <i>Lonicera japonica</i> on PM<sub>2.5</sub>-Induced Pulmonary Damage in BALB/c Mice via the TGF-β and NF-κB Pathway
This study aimed to assess the protective effect of an extract of <i>Lonicera japonica</i> against particulate-matter (PM)<sub>2.5</sub>-induced pulmonary inflammation and fibrosis. The compounds with physiological activity were identified as shanzhiside, secologanoside, loga...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/4/968 |
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| author | Hyo Lim Lee Jong Min Kim Min Ji Go Tae Yoon Kim Seung Gyum Joo Ju Hui Kim Han Su Lee Hyun-Jin Kim Ho Jin Heo |
| author_facet | Hyo Lim Lee Jong Min Kim Min Ji Go Tae Yoon Kim Seung Gyum Joo Ju Hui Kim Han Su Lee Hyun-Jin Kim Ho Jin Heo |
| author_sort | Hyo Lim Lee |
| collection | DOAJ |
| description | This study aimed to assess the protective effect of an extract of <i>Lonicera japonica</i> against particulate-matter (PM)<sub>2.5</sub>-induced pulmonary inflammation and fibrosis. The compounds with physiological activity were identified as shanzhiside, secologanoside, loganic acid, chlorogenic acid, secologanic acid, secoxyloganin, quercetin pentoside, and dicaffeoyl quinic acids (DCQA), including 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and 1,4-DCQA using ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS<sup>E</sup>). The extract of <i>Lonicera japonica</i> reduced cell death, reactive oxygen species (ROS) production, and inflammation in A549 cells. The extract of <i>Lonicera japonica</i> decreased serum T cells, including CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells, and total T helper 2 (Th2) cells, and immunoglobulins, including immunoglobulin G (IgG) and immunoglobulin E (IgE), in PM<sub>2.5</sub>-induced BALB/c mice. The extract of <i>Lonicera japonica</i> protected the pulmonary antioxidant system by regulating superoxide dismutase (SOD) activity, reduced glutathione (GSH) contents, and malondialdehyde (MDA) levels. In addition, it ameliorated mitochondrial function by regulating the production of ROS, mitochondrial membrane potential (MMP), and ATP contents. Moreover, the extract of <i>Lonicera japonica</i> exhibited a protective activity of apoptosis, fibrosis, and matrix metalloproteinases (MMPs) via TGF-β and NF-κB signaling pathways in lung tissues. This study suggests that the extract of <i>Lonicera japonica</i> might be a potential material to improve PM<sub>2.5</sub>-induced pulmonary inflammation, apoptosis, and fibrosis. |
| first_indexed | 2024-03-11T05:17:54Z |
| format | Article |
| id | doaj.art-58e31d3e09bf4e8fa7b4798fd6ffa67b |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T05:17:54Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-58e31d3e09bf4e8fa7b4798fd6ffa67b2023-11-17T18:06:58ZengMDPI AGAntioxidants2076-39212023-04-0112496810.3390/antiox12040968Protective Effect of <i>Lonicera japonica</i> on PM<sub>2.5</sub>-Induced Pulmonary Damage in BALB/c Mice via the TGF-β and NF-κB PathwayHyo Lim Lee0Jong Min Kim1Min Ji Go2Tae Yoon Kim3Seung Gyum Joo4Ju Hui Kim5Han Su Lee6Hyun-Jin Kim7Ho Jin Heo8Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaThis study aimed to assess the protective effect of an extract of <i>Lonicera japonica</i> against particulate-matter (PM)<sub>2.5</sub>-induced pulmonary inflammation and fibrosis. The compounds with physiological activity were identified as shanzhiside, secologanoside, loganic acid, chlorogenic acid, secologanic acid, secoxyloganin, quercetin pentoside, and dicaffeoyl quinic acids (DCQA), including 3,4-DCQA, 3,5-DCQA, 4,5-DCQA, and 1,4-DCQA using ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS<sup>E</sup>). The extract of <i>Lonicera japonica</i> reduced cell death, reactive oxygen species (ROS) production, and inflammation in A549 cells. The extract of <i>Lonicera japonica</i> decreased serum T cells, including CD4<sup>+</sup> T cells, CD8<sup>+</sup> T cells, and total T helper 2 (Th2) cells, and immunoglobulins, including immunoglobulin G (IgG) and immunoglobulin E (IgE), in PM<sub>2.5</sub>-induced BALB/c mice. The extract of <i>Lonicera japonica</i> protected the pulmonary antioxidant system by regulating superoxide dismutase (SOD) activity, reduced glutathione (GSH) contents, and malondialdehyde (MDA) levels. In addition, it ameliorated mitochondrial function by regulating the production of ROS, mitochondrial membrane potential (MMP), and ATP contents. Moreover, the extract of <i>Lonicera japonica</i> exhibited a protective activity of apoptosis, fibrosis, and matrix metalloproteinases (MMPs) via TGF-β and NF-κB signaling pathways in lung tissues. This study suggests that the extract of <i>Lonicera japonica</i> might be a potential material to improve PM<sub>2.5</sub>-induced pulmonary inflammation, apoptosis, and fibrosis.https://www.mdpi.com/2076-3921/12/4/968<i>Lonicera japonica</i>PM<sub>2.5</sub>pulmonary diseaseinflammationfibrosis |
| spellingShingle | Hyo Lim Lee Jong Min Kim Min Ji Go Tae Yoon Kim Seung Gyum Joo Ju Hui Kim Han Su Lee Hyun-Jin Kim Ho Jin Heo Protective Effect of <i>Lonicera japonica</i> on PM<sub>2.5</sub>-Induced Pulmonary Damage in BALB/c Mice via the TGF-β and NF-κB Pathway Antioxidants <i>Lonicera japonica</i> PM<sub>2.5</sub> pulmonary disease inflammation fibrosis |
| title | Protective Effect of <i>Lonicera japonica</i> on PM<sub>2.5</sub>-Induced Pulmonary Damage in BALB/c Mice via the TGF-β and NF-κB Pathway |
| title_full | Protective Effect of <i>Lonicera japonica</i> on PM<sub>2.5</sub>-Induced Pulmonary Damage in BALB/c Mice via the TGF-β and NF-κB Pathway |
| title_fullStr | Protective Effect of <i>Lonicera japonica</i> on PM<sub>2.5</sub>-Induced Pulmonary Damage in BALB/c Mice via the TGF-β and NF-κB Pathway |
| title_full_unstemmed | Protective Effect of <i>Lonicera japonica</i> on PM<sub>2.5</sub>-Induced Pulmonary Damage in BALB/c Mice via the TGF-β and NF-κB Pathway |
| title_short | Protective Effect of <i>Lonicera japonica</i> on PM<sub>2.5</sub>-Induced Pulmonary Damage in BALB/c Mice via the TGF-β and NF-κB Pathway |
| title_sort | protective effect of i lonicera japonica i on pm sub 2 5 sub induced pulmonary damage in balb c mice via the tgf β and nf κb pathway |
| topic | <i>Lonicera japonica</i> PM<sub>2.5</sub> pulmonary disease inflammation fibrosis |
| url | https://www.mdpi.com/2076-3921/12/4/968 |
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