Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report
Within the EU-SPIDIA project (www.spidia.eu), the quality parameters of blood genomic DNA were defined [SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses – [1]; Influence of pre-analytical procedures on genomic DNA integrity in blood...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340916300142 |
| _version_ | 1828876371463503872 |
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| author | Francesca Malentacchi Sara Pizzamiglio Hady Ibrahim-Gawel Mario Pazzagli Paolo Verderio Chiara Maura Ciniselli Ralf Wyrich Stefania Gelmini |
| author_facet | Francesca Malentacchi Sara Pizzamiglio Hady Ibrahim-Gawel Mario Pazzagli Paolo Verderio Chiara Maura Ciniselli Ralf Wyrich Stefania Gelmini |
| author_sort | Francesca Malentacchi |
| collection | DOAJ |
| description | Within the EU-SPIDIA project (www.spidia.eu), the quality parameters of blood genomic DNA were defined [SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses – [1]; Influence of pre-analytical procedures on genomic DNA integrity in blood samples: the SPIDIA experience – [2]; Combining qualitative and quantitative imaging evaluation for the assessment of genomic DNA integrity: the SPIDIA experience – [3]. DNA quality parameters were used to evaluate the laboratory performance within an External Quality Assessment (EQA) [Second SPIDIA-DNA External Quality Assessment (EQA): Influence of pre-analytical phase of blood samples on genomic DNA quality – [4]. These parameters included DNA purity and yield by UV spectrophotometric measurements, the presence of PCR interferences by Kineret software and genomic DNA integrity analysis by Pulsed Field Gel Electrophoresis.Here we present the specific laboratory report of the 2nd SPIDIA-DNA EQA as an example of data and performances evaluation. |
| first_indexed | 2024-12-13T08:20:01Z |
| format | Article |
| id | doaj.art-58e50c60c5804a7996661d8a87f0154e |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-13T08:20:01Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-58e50c60c5804a7996661d8a87f0154e2022-12-21T23:54:02ZengElsevierData in Brief2352-34092016-03-016980984Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory reportFrancesca Malentacchi0Sara Pizzamiglio1Hady Ibrahim-Gawel2Mario Pazzagli3Paolo Verderio4Chiara Maura Ciniselli5Ralf Wyrich6Stefania Gelmini7Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Italy; Corresponding author at: Department of Biomedical, Experimental and Clinical Sciences, University of Florence, Viale G. Pieraccini, 6–50139 Florence, Italy. Tel.: +0039 055 2758251; fax: +0039 055 2758261.Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, ItalyQIAGEN GmbH, Hilden, GermanyDepartment of Biomedical, Experimental and Clinical Sciences, University of Florence, ItalyFondazione IRCCS Istituto Nazionale dei Tumori, Milan, ItalyFondazione IRCCS Istituto Nazionale dei Tumori, Milan, ItalyQIAGEN GmbH, Hilden, GermanyDepartment of Biomedical, Experimental and Clinical Sciences, University of Florence, ItalyWithin the EU-SPIDIA project (www.spidia.eu), the quality parameters of blood genomic DNA were defined [SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses – [1]; Influence of pre-analytical procedures on genomic DNA integrity in blood samples: the SPIDIA experience – [2]; Combining qualitative and quantitative imaging evaluation for the assessment of genomic DNA integrity: the SPIDIA experience – [3]. DNA quality parameters were used to evaluate the laboratory performance within an External Quality Assessment (EQA) [Second SPIDIA-DNA External Quality Assessment (EQA): Influence of pre-analytical phase of blood samples on genomic DNA quality – [4]. These parameters included DNA purity and yield by UV spectrophotometric measurements, the presence of PCR interferences by Kineret software and genomic DNA integrity analysis by Pulsed Field Gel Electrophoresis.Here we present the specific laboratory report of the 2nd SPIDIA-DNA EQA as an example of data and performances evaluation.http://www.sciencedirect.com/science/article/pii/S2352340916300142 |
| spellingShingle | Francesca Malentacchi Sara Pizzamiglio Hady Ibrahim-Gawel Mario Pazzagli Paolo Verderio Chiara Maura Ciniselli Ralf Wyrich Stefania Gelmini Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report Data in Brief |
| title | Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report |
| title_full | Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report |
| title_fullStr | Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report |
| title_full_unstemmed | Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report |
| title_short | Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report |
| title_sort | data and performances evaluation of the spidia dna pan european external quality assessment 2nd spidia dna laboratory report |
| url | http://www.sciencedirect.com/science/article/pii/S2352340916300142 |
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