CELL-MEDIATED IMMUNITY IN PATIENTS WITH POSTTRAUMATIC LONG BONE OSTEOMYELITIS IN THE COURSE OF MANAGEMENT WITH THE ILIZAROV METHOD
Cell-mediated immunity parameters were studied in 26 patients (13 males, 13 females) aged 46 years (range: 39-51 years) with fistular type of chronic posttraumatic osteomyelitis of the humerus during the course of surgical treatment with the Ilizarov monofocal transosseous osteosynthesis. The study...
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| Format: | Article |
| Language: | Russian |
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Russian Academy of Sciences, Siberian Branch Publishing House
2019-02-01
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| Series: | Сибирский научный медицинский журнал |
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| Online Access: | https://sibmed.elpub.ru/jour/article/view/85 |
| _version_ | 1826575755910316032 |
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| author | M. V. Chepeleva N. M. Klyushin D. S. Leonchuk E. I. Kuznetsova |
| author_facet | M. V. Chepeleva N. M. Klyushin D. S. Leonchuk E. I. Kuznetsova |
| author_sort | M. V. Chepeleva |
| collection | DOAJ |
| description | Cell-mediated immunity parameters were studied in 26 patients (13 males, 13 females) aged 46 years (range: 39-51 years) with fistular type of chronic posttraumatic osteomyelitis of the humerus during the course of surgical treatment with the Ilizarov monofocal transosseous osteosynthesis. The study time-points were pre-surgery period and the following treatment stages: days 3, 7, 21 after osteosynthesis and day 1 after removal of the device. An obligatory criterion of inclusion was absence of postoperative complications and infection recurrence at least a year after removal of the apparatus. Control group were 30 healthy volunteers in the similar age (15 males, 15 females). Carriers of HCV, HIV, HBsAg and persons with concomitant somatic pathology that could affect the results of immunological study were not included. The study of subpopulation composition of the peripheral blood lymphocytes was carried out using laser flow cytometry. The parameters of cell-mediated immunity in patients with a fistular type of chronic posttraumatic osteomyelitis of the humerus were characterized by mild lymphopenia, increase in the number of cells of early and late activation of T lymphocytes (CD3+CD25+, CD3+HLA-DR+). Physiological response of the immune system to debridement of the purulent foci and transosseous osteosynthesis was normalization of the number of activated T-lymphocytes at the final stage of surgical treatment. In general, the dynamic changes of immunological indices were in favor of persistent arrest of the infection process and were confirmed by clinical observations. |
| first_indexed | 2024-03-08T14:33:53Z |
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| id | doaj.art-58ec5bdd837e48fbaf60e541fe736b36 |
| institution | Directory Open Access Journal |
| issn | 2410-2512 2410-2520 |
| language | Russian |
| last_indexed | 2025-03-14T13:19:45Z |
| publishDate | 2019-02-01 |
| publisher | Russian Academy of Sciences, Siberian Branch Publishing House |
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| series | Сибирский научный медицинский журнал |
| spelling | doaj.art-58ec5bdd837e48fbaf60e541fe736b362025-02-28T13:43:53ZrusRussian Academy of Sciences, Siberian Branch Publishing HouseСибирский научный медицинский журнал2410-25122410-25202019-02-01386646910.15372/SSMJ2018061085CELL-MEDIATED IMMUNITY IN PATIENTS WITH POSTTRAUMATIC LONG BONE OSTEOMYELITIS IN THE COURSE OF MANAGEMENT WITH THE ILIZAROV METHODM. V. Chepeleva0N. M. Klyushin1D. S. Leonchuk2E. I. Kuznetsova3Russian Ilizarov Scientific Centre «Restorative Traumatology and Orthopaedics» of Minzdrav of RussiaRussian Ilizarov Scientific Centre «Restorative Traumatology and Orthopaedics» of Minzdrav of RussiaRussian Ilizarov Scientific Centre «Restorative Traumatology and Orthopaedics» of Minzdrav of RussiaRussian Ilizarov Scientific Centre «Restorative Traumatology and Orthopaedics» of Minzdrav of RussiaCell-mediated immunity parameters were studied in 26 patients (13 males, 13 females) aged 46 years (range: 39-51 years) with fistular type of chronic posttraumatic osteomyelitis of the humerus during the course of surgical treatment with the Ilizarov monofocal transosseous osteosynthesis. The study time-points were pre-surgery period and the following treatment stages: days 3, 7, 21 after osteosynthesis and day 1 after removal of the device. An obligatory criterion of inclusion was absence of postoperative complications and infection recurrence at least a year after removal of the apparatus. Control group were 30 healthy volunteers in the similar age (15 males, 15 females). Carriers of HCV, HIV, HBsAg and persons with concomitant somatic pathology that could affect the results of immunological study were not included. The study of subpopulation composition of the peripheral blood lymphocytes was carried out using laser flow cytometry. The parameters of cell-mediated immunity in patients with a fistular type of chronic posttraumatic osteomyelitis of the humerus were characterized by mild lymphopenia, increase in the number of cells of early and late activation of T lymphocytes (CD3+CD25+, CD3+HLA-DR+). Physiological response of the immune system to debridement of the purulent foci and transosseous osteosynthesis was normalization of the number of activated T-lymphocytes at the final stage of surgical treatment. In general, the dynamic changes of immunological indices were in favor of persistent arrest of the infection process and were confirmed by clinical observations.https://sibmed.elpub.ru/jour/article/view/85cd3<sup>+</sup>cd19<sup>-</sup>cd3<sup>-</sup>cd19<sup>+</sup>cd3<sup>+</sup>cd4<sup>+</sup>cd3<sup>+</sup>cd8<sup>+</sup>cd3<sup>+</sup>cd25<sup>+</sup>cd3<sup>+</sup>hla-dr<sup>+</sup>posttraumatic osteomyelitisosteosynthesisflow cytometrycell-mediated immunitycd3<sup>+</sup>cd19<sup>-</sup>cd3<sup>- </sup>cd19<sup>+</sup>cd3<sup>+</sup>cd4<sup>+</sup>cd3<sup>+</sup>cd8<sup>+</sup>cd3<sup>+</sup>cd25<sup>+</sup>cd3<sup>+</sup>hla-dr<sup>+</sup> |
| spellingShingle | M. V. Chepeleva N. M. Klyushin D. S. Leonchuk E. I. Kuznetsova CELL-MEDIATED IMMUNITY IN PATIENTS WITH POSTTRAUMATIC LONG BONE OSTEOMYELITIS IN THE COURSE OF MANAGEMENT WITH THE ILIZAROV METHOD Сибирский научный медицинский журнал cd3<sup>+</sup>cd19<sup>-</sup> cd3<sup>-</sup>cd19<sup>+</sup> cd3<sup>+</sup>cd4<sup>+</sup> cd3<sup>+</sup>cd8<sup>+</sup> cd3<sup>+</sup>cd25<sup>+</sup> cd3<sup>+</sup>hla-dr<sup>+</sup> posttraumatic osteomyelitis osteosynthesis flow cytometry cell-mediated immunity cd3<sup>+</sup>cd19<sup>-</sup> cd3<sup>- </sup>cd19<sup>+</sup> cd3<sup>+</sup>cd4<sup>+</sup> cd3<sup>+</sup>cd8<sup>+</sup> cd3<sup>+</sup>cd25<sup>+</sup> cd3<sup>+</sup>hla-dr<sup>+</sup> |
| title | CELL-MEDIATED IMMUNITY IN PATIENTS WITH POSTTRAUMATIC LONG BONE OSTEOMYELITIS IN THE COURSE OF MANAGEMENT WITH THE ILIZAROV METHOD |
| title_full | CELL-MEDIATED IMMUNITY IN PATIENTS WITH POSTTRAUMATIC LONG BONE OSTEOMYELITIS IN THE COURSE OF MANAGEMENT WITH THE ILIZAROV METHOD |
| title_fullStr | CELL-MEDIATED IMMUNITY IN PATIENTS WITH POSTTRAUMATIC LONG BONE OSTEOMYELITIS IN THE COURSE OF MANAGEMENT WITH THE ILIZAROV METHOD |
| title_full_unstemmed | CELL-MEDIATED IMMUNITY IN PATIENTS WITH POSTTRAUMATIC LONG BONE OSTEOMYELITIS IN THE COURSE OF MANAGEMENT WITH THE ILIZAROV METHOD |
| title_short | CELL-MEDIATED IMMUNITY IN PATIENTS WITH POSTTRAUMATIC LONG BONE OSTEOMYELITIS IN THE COURSE OF MANAGEMENT WITH THE ILIZAROV METHOD |
| title_sort | cell mediated immunity in patients with posttraumatic long bone osteomyelitis in the course of management with the ilizarov method |
| topic | cd3<sup>+</sup>cd19<sup>-</sup> cd3<sup>-</sup>cd19<sup>+</sup> cd3<sup>+</sup>cd4<sup>+</sup> cd3<sup>+</sup>cd8<sup>+</sup> cd3<sup>+</sup>cd25<sup>+</sup> cd3<sup>+</sup>hla-dr<sup>+</sup> posttraumatic osteomyelitis osteosynthesis flow cytometry cell-mediated immunity cd3<sup>+</sup>cd19<sup>-</sup> cd3<sup>- </sup>cd19<sup>+</sup> cd3<sup>+</sup>cd4<sup>+</sup> cd3<sup>+</sup>cd8<sup>+</sup> cd3<sup>+</sup>cd25<sup>+</sup> cd3<sup>+</sup>hla-dr<sup>+</sup> |
| url | https://sibmed.elpub.ru/jour/article/view/85 |
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