Composition of the alfalfa pathobiome in commercial fields
Through the recent advances of modern high-throughput sequencing technologies, the “one microbe, one disease” dogma is being gradually replaced with the principle of the “pathobiome”. Pathobiome is a comprehensive biotic environment that not only includes a diverse community of all disease-causing o...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1225781/full |
| _version_ | 1797737483507597312 |
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| author | Lev G. Nemchinov Brian M. Irish Igor V. Uschapovsky Sam Grinstead Jonathan Shao Olga A. Postnikova Olga A. Postnikova |
| author_facet | Lev G. Nemchinov Brian M. Irish Igor V. Uschapovsky Sam Grinstead Jonathan Shao Olga A. Postnikova Olga A. Postnikova |
| author_sort | Lev G. Nemchinov |
| collection | DOAJ |
| description | Through the recent advances of modern high-throughput sequencing technologies, the “one microbe, one disease” dogma is being gradually replaced with the principle of the “pathobiome”. Pathobiome is a comprehensive biotic environment that not only includes a diverse community of all disease-causing organisms within the plant but also defines their mutual interactions and resultant effect on plant health. To date, the concept of pathobiome as a major component in plant health and sustainable production of alfalfa (Medicago sativa L.), the most extensively cultivated forage legume in the world, is non-existent. Here, we approached this subject by characterizing the biodiversity of the alfalfa pathobiome using high-throughput sequencing technology. Our metagenomic study revealed a remarkable abundance of different pathogenic communities associated with alfalfa in the natural ecosystem. Profiling the alfalfa pathobiome is a starting point to assess known and identify new and emerging stress challenges in the context of plant disease management. In addition, it allows us to address the complexity of microbial interactions within the plant host and their impact on the development and evolution of pathogenesis. |
| first_indexed | 2024-03-12T13:29:25Z |
| format | Article |
| id | doaj.art-58ef33c46d9e480193b4403fa82b8809 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T13:29:25Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-58ef33c46d9e480193b4403fa82b88092023-08-24T18:53:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12257811225781Composition of the alfalfa pathobiome in commercial fieldsLev G. Nemchinov0Brian M. Irish1Igor V. Uschapovsky2Sam Grinstead3Jonathan Shao4Olga A. Postnikova5Olga A. Postnikova6Molecular Plant Pathology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, United StatesPlant Germplasm Introduction and Testing Research Unit, Prosser, WA, United StatesFederal Research Center for Bast Fiber Crops, Tver, RussiaMolecular Plant Pathology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, United StatesUnited States Department of Agriculture, Agricultural Research Service, Office of The Area Director, Beltsville, MD, United StatesMolecular Plant Pathology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, United StatesAnimal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Center, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD, United StatesThrough the recent advances of modern high-throughput sequencing technologies, the “one microbe, one disease” dogma is being gradually replaced with the principle of the “pathobiome”. Pathobiome is a comprehensive biotic environment that not only includes a diverse community of all disease-causing organisms within the plant but also defines their mutual interactions and resultant effect on plant health. To date, the concept of pathobiome as a major component in plant health and sustainable production of alfalfa (Medicago sativa L.), the most extensively cultivated forage legume in the world, is non-existent. Here, we approached this subject by characterizing the biodiversity of the alfalfa pathobiome using high-throughput sequencing technology. Our metagenomic study revealed a remarkable abundance of different pathogenic communities associated with alfalfa in the natural ecosystem. Profiling the alfalfa pathobiome is a starting point to assess known and identify new and emerging stress challenges in the context of plant disease management. In addition, it allows us to address the complexity of microbial interactions within the plant host and their impact on the development and evolution of pathogenesis.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1225781/fullpathobiomealfalfaMedicago sativahigh-throughput sequencingvirusesbacteria |
| spellingShingle | Lev G. Nemchinov Brian M. Irish Igor V. Uschapovsky Sam Grinstead Jonathan Shao Olga A. Postnikova Olga A. Postnikova Composition of the alfalfa pathobiome in commercial fields Frontiers in Microbiology pathobiome alfalfa Medicago sativa high-throughput sequencing viruses bacteria |
| title | Composition of the alfalfa pathobiome in commercial fields |
| title_full | Composition of the alfalfa pathobiome in commercial fields |
| title_fullStr | Composition of the alfalfa pathobiome in commercial fields |
| title_full_unstemmed | Composition of the alfalfa pathobiome in commercial fields |
| title_short | Composition of the alfalfa pathobiome in commercial fields |
| title_sort | composition of the alfalfa pathobiome in commercial fields |
| topic | pathobiome alfalfa Medicago sativa high-throughput sequencing viruses bacteria |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1225781/full |
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