Neurosecretory Protein GL Accelerates Liver Steatosis in Mice Fed Medium-Fat/Medium-Fructose Diet
Sugar consumption can readily lead to obesity and metabolic diseases such as liver steatosis. We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats. However, differences in lipogenic effici...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/4/2071 |
| _version_ | 1797479374487814144 |
|---|---|
| author | Yuki Narimatsu Eiko Iwakoshi-Ukena Mana Naito Shogo Moriwaki Megumi Furumitsu Kazuyoshi Ukena |
| author_facet | Yuki Narimatsu Eiko Iwakoshi-Ukena Mana Naito Shogo Moriwaki Megumi Furumitsu Kazuyoshi Ukena |
| author_sort | Yuki Narimatsu |
| collection | DOAJ |
| description | Sugar consumption can readily lead to obesity and metabolic diseases such as liver steatosis. We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats. However, differences in lipogenic efficiency of sugar types by NPGL remain unclear. The present study aimed to elucidate the obesogenic effects of NPGL on mice fed different sugars (i.e., sucrose or fructose). We overexpressed the NPGL-precursor gene (<i>Npgl</i>) in the hypothalamus of mice fed a medium-fat/medium-sucrose diet (MFSD) or a medium-fat/medium-fructose diet (MFFD). Food intake and body mass were measured for 28 days. Body composition and mRNA expression of lipid metabolic factors were measured at the endpoint. <i>Npgl</i> overexpression potently increased body mass with fat accumulation in the white adipose tissue of mice fed MFFD, although it did not markedly affect food intake. In contrast, we observed profound fat deposition in the livers of mice fed MFFD but not MFSD. In the liver, the mRNA expression of glucose and lipid metabolic factors was affected in mice fed MFFD. Hence, NPGL induced liver steatosis in mice fed a fructose-rich diet. |
| first_indexed | 2024-03-09T21:44:57Z |
| format | Article |
| id | doaj.art-58eff8d4c4ab4fdda2731f197213ef60 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:44:57Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-58eff8d4c4ab4fdda2731f197213ef602023-11-23T20:19:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-02-01234207110.3390/ijms23042071Neurosecretory Protein GL Accelerates Liver Steatosis in Mice Fed Medium-Fat/Medium-Fructose DietYuki Narimatsu0Eiko Iwakoshi-Ukena1Mana Naito2Shogo Moriwaki3Megumi Furumitsu4Kazuyoshi Ukena5Laboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanLaboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanLaboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanLaboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanLaboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanLaboratory of Neurometabolism, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8521, JapanSugar consumption can readily lead to obesity and metabolic diseases such as liver steatosis. We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats. However, differences in lipogenic efficiency of sugar types by NPGL remain unclear. The present study aimed to elucidate the obesogenic effects of NPGL on mice fed different sugars (i.e., sucrose or fructose). We overexpressed the NPGL-precursor gene (<i>Npgl</i>) in the hypothalamus of mice fed a medium-fat/medium-sucrose diet (MFSD) or a medium-fat/medium-fructose diet (MFFD). Food intake and body mass were measured for 28 days. Body composition and mRNA expression of lipid metabolic factors were measured at the endpoint. <i>Npgl</i> overexpression potently increased body mass with fat accumulation in the white adipose tissue of mice fed MFFD, although it did not markedly affect food intake. In contrast, we observed profound fat deposition in the livers of mice fed MFFD but not MFSD. In the liver, the mRNA expression of glucose and lipid metabolic factors was affected in mice fed MFFD. Hence, NPGL induced liver steatosis in mice fed a fructose-rich diet.https://www.mdpi.com/1422-0067/23/4/2071neurosecretory protein GLhypothalamusneuropeptideobesitydietary sugarliver steatosis |
| spellingShingle | Yuki Narimatsu Eiko Iwakoshi-Ukena Mana Naito Shogo Moriwaki Megumi Furumitsu Kazuyoshi Ukena Neurosecretory Protein GL Accelerates Liver Steatosis in Mice Fed Medium-Fat/Medium-Fructose Diet International Journal of Molecular Sciences neurosecretory protein GL hypothalamus neuropeptide obesity dietary sugar liver steatosis |
| title | Neurosecretory Protein GL Accelerates Liver Steatosis in Mice Fed Medium-Fat/Medium-Fructose Diet |
| title_full | Neurosecretory Protein GL Accelerates Liver Steatosis in Mice Fed Medium-Fat/Medium-Fructose Diet |
| title_fullStr | Neurosecretory Protein GL Accelerates Liver Steatosis in Mice Fed Medium-Fat/Medium-Fructose Diet |
| title_full_unstemmed | Neurosecretory Protein GL Accelerates Liver Steatosis in Mice Fed Medium-Fat/Medium-Fructose Diet |
| title_short | Neurosecretory Protein GL Accelerates Liver Steatosis in Mice Fed Medium-Fat/Medium-Fructose Diet |
| title_sort | neurosecretory protein gl accelerates liver steatosis in mice fed medium fat medium fructose diet |
| topic | neurosecretory protein GL hypothalamus neuropeptide obesity dietary sugar liver steatosis |
| url | https://www.mdpi.com/1422-0067/23/4/2071 |
| work_keys_str_mv | AT yukinarimatsu neurosecretoryproteinglacceleratesliversteatosisinmicefedmediumfatmediumfructosediet AT eikoiwakoshiukena neurosecretoryproteinglacceleratesliversteatosisinmicefedmediumfatmediumfructosediet AT mananaito neurosecretoryproteinglacceleratesliversteatosisinmicefedmediumfatmediumfructosediet AT shogomoriwaki neurosecretoryproteinglacceleratesliversteatosisinmicefedmediumfatmediumfructosediet AT megumifurumitsu neurosecretoryproteinglacceleratesliversteatosisinmicefedmediumfatmediumfructosediet AT kazuyoshiukena neurosecretoryproteinglacceleratesliversteatosisinmicefedmediumfatmediumfructosediet |