High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from ‘Candidatus Liberibacter asiaticus’
Two miniature inverted-repeat transposable elements (MITEs), MCLas-A and MCLas-B, were recently identified from ‘Candidatus Liberibacter asiaticus’ known to be associated with citrus Huanglongbing (HLB, yellow shoot disease). MCLas-A was suggested as an active MITE because of its mobility. The immed...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311914609795 |
| _version_ | 1818865352333852672 |
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| author | Xue-feng WANG Jiao-yue CHEN Jin TAN Suo DUAN Xiao-ling DENG Jian-chi CHEN Chang-yong ZHOU |
| author_facet | Xue-feng WANG Jiao-yue CHEN Jin TAN Suo DUAN Xiao-ling DENG Jian-chi CHEN Chang-yong ZHOU |
| author_sort | Xue-feng WANG |
| collection | DOAJ |
| description | Two miniature inverted-repeat transposable elements (MITEs), MCLas-A and MCLas-B, were recently identified from ‘Candidatus Liberibacter asiaticus’ known to be associated with citrus Huanglongbing (HLB, yellow shoot disease). MCLas-A was suggested as an active MITE because of its mobility. The immediate upstream gene of the two MITEs was predicted to be a putative transposase. The goal of this study is to analyze the sequence variation in the upstream putative transposase of MITEs and explore the possible correlation between sequence variation of transposase gene and MITE activity. PCR and sequence analysis showed that 12 sequence types were found in six major amplicon types from 43 representative ‘Ca. L. asiaticus’ isolates from China, the United States and Brazil. Out of the 12 sequence types, three (T4, T5-2, T6) were reported for the first time. Recombination events were found in the two unique sequence types (T5-2 and T6) which were detected in all Brazilian isolates. Notably, no sequence variation or recombination events were detected in the upstream putative transposase gene of MCLas-A, suggesting the conservation of the transposase gene might be closely related with the MITE activity. Phylogenetic analysis demonstrated two well supported clades including five subclades were identified, clearly reflecting the geographical origins of isolates, especially that of Ruili isolates, São Paulo isolates and a few Florida isolates. |
| first_indexed | 2024-12-19T10:46:11Z |
| format | Article |
| id | doaj.art-58f2b6dc85ec4067b67356d72f66bf41 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-19T10:46:11Z |
| publishDate | 2015-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-58f2b6dc85ec4067b67356d72f66bf412022-12-21T20:25:15ZengElsevierJournal of Integrative Agriculture2095-31192015-10-01141020022010High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from ‘Candidatus Liberibacter asiaticus’Xue-feng WANG0Jiao-yue CHEN1Jin TAN2Suo DUAN3Xiao-ling DENG4Jian-chi CHEN5Chang-yong ZHOU6National Citrus Engineering Research Center, Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University, Chongqing 400712, P.R. ChinaNational Citrus Engineering Research Center, Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University, Chongqing 400712, P.R. China; College of Plant Protection, Southwest University, Chongqing 400715, P.R. ChinaNational Citrus Engineering Research Center, Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University, Chongqing 400712, P.R. ChinaNational Citrus Engineering Research Center, Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University, Chongqing 400712, P.R. China; College of Plant Protection, Southwest University, Chongqing 400715, P.R. ChinaCitrus Huanglongbing Research Center, South China Agricultural University, Guangzhou 510642, P.R. ChinaSan Joaquin Valley Agricultural Sciences Center (SJVASC), USDA-ARS, Parlier, CA 93648, USANational Citrus Engineering Research Center, Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University, Chongqing 400712, P.R. China; Correspondence ZHOU Chang-yong, Tel: +86-23-68349601Two miniature inverted-repeat transposable elements (MITEs), MCLas-A and MCLas-B, were recently identified from ‘Candidatus Liberibacter asiaticus’ known to be associated with citrus Huanglongbing (HLB, yellow shoot disease). MCLas-A was suggested as an active MITE because of its mobility. The immediate upstream gene of the two MITEs was predicted to be a putative transposase. The goal of this study is to analyze the sequence variation in the upstream putative transposase of MITEs and explore the possible correlation between sequence variation of transposase gene and MITE activity. PCR and sequence analysis showed that 12 sequence types were found in six major amplicon types from 43 representative ‘Ca. L. asiaticus’ isolates from China, the United States and Brazil. Out of the 12 sequence types, three (T4, T5-2, T6) were reported for the first time. Recombination events were found in the two unique sequence types (T5-2 and T6) which were detected in all Brazilian isolates. Notably, no sequence variation or recombination events were detected in the upstream putative transposase gene of MCLas-A, suggesting the conservation of the transposase gene might be closely related with the MITE activity. Phylogenetic analysis demonstrated two well supported clades including five subclades were identified, clearly reflecting the geographical origins of isolates, especially that of Ruili isolates, São Paulo isolates and a few Florida isolates.http://www.sciencedirect.com/science/article/pii/S2095311914609795Huanglongbing‘Candidatus Liberibacter asiaticus’miniature inverted-repeat transposable element |
| spellingShingle | Xue-feng WANG Jiao-yue CHEN Jin TAN Suo DUAN Xiao-ling DENG Jian-chi CHEN Chang-yong ZHOU High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from ‘Candidatus Liberibacter asiaticus’ Journal of Integrative Agriculture Huanglongbing ‘Candidatus Liberibacter asiaticus’ miniature inverted-repeat transposable element |
| title | High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from ‘Candidatus Liberibacter asiaticus’ |
| title_full | High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from ‘Candidatus Liberibacter asiaticus’ |
| title_fullStr | High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from ‘Candidatus Liberibacter asiaticus’ |
| title_full_unstemmed | High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from ‘Candidatus Liberibacter asiaticus’ |
| title_short | High genetic variation and recombination events in the vicinity of non-autonomous transposable elements from ‘Candidatus Liberibacter asiaticus’ |
| title_sort | high genetic variation and recombination events in the vicinity of non autonomous transposable elements from candidatus liberibacter asiaticus |
| topic | Huanglongbing ‘Candidatus Liberibacter asiaticus’ miniature inverted-repeat transposable element |
| url | http://www.sciencedirect.com/science/article/pii/S2095311914609795 |
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