Agreement of three commercial anti-extractable nuclear antigen tests: EUROASSAY Anti-ENA Profile, Polycheck Autoimmune Test and FID Connective Profile
BackgroundDetection of antibodies to extractable nuclear antigens (ENAs) is needed for the diagnosis in systemic autoimmune diseases. In this study, we compared three reagents using line immunoblot assay (LIA) or multiplex bead immunoassay for detecting the anti-ENAs. MethodsA total of 89 sera were...
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Kosin University College of Medicine
2018-01-01
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| Series: | Kosin Medical Journal |
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| Online Access: | http://www.kosinmedj.org/upload/pdf/ksmc033-03-04.pdf |
| _version_ | 1811192736022265856 |
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| author | Namhee Kim In-Suk Kim Chulhun L Chang Hyung-Hoi Kim Eun Yup Lee |
| author_facet | Namhee Kim In-Suk Kim Chulhun L Chang Hyung-Hoi Kim Eun Yup Lee |
| author_sort | Namhee Kim |
| collection | DOAJ |
| description | BackgroundDetection of antibodies to extractable nuclear antigens (ENAs) is needed for the diagnosis in systemic autoimmune diseases. In this study, we compared three reagents using line immunoblot assay (LIA) or multiplex bead immunoassay for detecting the anti-ENAs. MethodsA total of 89 sera were tested by 3 different assays: EUROASSAY Anti-ENA Profile (Euroimmune, Germany), Polycheck Autoimmune Test (Biocheck GmbH, Germany), and FIDIS™ Connective Profile (Biomedical Diagnostics, France). The following individual ENAs were investigated: Sm, SS-A (Ro), SS-B (La), Scl-70, Jo-1 and RNP. We reviewed medical records to investigate the discrepant results among three methods. ResultsOverall percent agreements were 96.1% between EUROASSAY Anti-ENA Profile and FIDIS™ Connective profile; 90.4% between EUROASSAY Anti-ENA Profile and Polycheck Autoimmune Test using the manufacturers’ cutoff; 96.4% between EUROASSAY Anti-ENA Profile and Polycheck Autoimmune Test using a upward cutoff; 90.4% between FIDIS™ Connective profile and Polycheck Autoimmune Test the manufacturers’ cutoff; and 96.4% between FIDIS™ Connective profile and Polycheck Autoimmune Test a upward cutoff. ConclusionsThe three assays showed excellent agreement with each other. With appropriate cutoff, the all three assays for six of the anti-ENA tests investigated in this study can be used in clinical laboratories for detecting the anti-ENAs. |
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| institution | Directory Open Access Journal |
| issn | 2005-9531 2586-7024 |
| language | English |
| last_indexed | 2024-04-11T23:56:18Z |
| publishDate | 2018-01-01 |
| publisher | Kosin University College of Medicine |
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| series | Kosin Medical Journal |
| spelling | doaj.art-58f3f9ca27db4938a7aaee2c25c907d72022-12-22T03:56:21ZengKosin University College of MedicineKosin Medical Journal2005-95312586-70242018-01-0133330731710.7180/kmj.2018.33.3.307209Agreement of three commercial anti-extractable nuclear antigen tests: EUROASSAY Anti-ENA Profile, Polycheck Autoimmune Test and FID Connective ProfileNamhee Kim0In-Suk Kim1Chulhun L Chang2Hyung-Hoi Kim3Eun Yup Lee4Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, KoreaDepartment of Laboratory Medicine, School of Medicine, Pusan National University, Busan, KoreaDepartment of Laboratory Medicine, School of Medicine, Pusan National University, Busan, KoreaDepartment of Laboratory Medicine, School of Medicine, Pusan National University, Busan, KoreaDepartment of Laboratory Medicine, School of Medicine, Pusan National University, Busan, KoreaBackgroundDetection of antibodies to extractable nuclear antigens (ENAs) is needed for the diagnosis in systemic autoimmune diseases. In this study, we compared three reagents using line immunoblot assay (LIA) or multiplex bead immunoassay for detecting the anti-ENAs. MethodsA total of 89 sera were tested by 3 different assays: EUROASSAY Anti-ENA Profile (Euroimmune, Germany), Polycheck Autoimmune Test (Biocheck GmbH, Germany), and FIDIS™ Connective Profile (Biomedical Diagnostics, France). The following individual ENAs were investigated: Sm, SS-A (Ro), SS-B (La), Scl-70, Jo-1 and RNP. We reviewed medical records to investigate the discrepant results among three methods. ResultsOverall percent agreements were 96.1% between EUROASSAY Anti-ENA Profile and FIDIS™ Connective profile; 90.4% between EUROASSAY Anti-ENA Profile and Polycheck Autoimmune Test using the manufacturers’ cutoff; 96.4% between EUROASSAY Anti-ENA Profile and Polycheck Autoimmune Test using a upward cutoff; 90.4% between FIDIS™ Connective profile and Polycheck Autoimmune Test the manufacturers’ cutoff; and 96.4% between FIDIS™ Connective profile and Polycheck Autoimmune Test a upward cutoff. ConclusionsThe three assays showed excellent agreement with each other. With appropriate cutoff, the all three assays for six of the anti-ENA tests investigated in this study can be used in clinical laboratories for detecting the anti-ENAs.http://www.kosinmedj.org/upload/pdf/ksmc033-03-04.pdfanti-extractable nuclear antigen antibodiesantinuclear antibodiesautoimmunity |
| spellingShingle | Namhee Kim In-Suk Kim Chulhun L Chang Hyung-Hoi Kim Eun Yup Lee Agreement of three commercial anti-extractable nuclear antigen tests: EUROASSAY Anti-ENA Profile, Polycheck Autoimmune Test and FID Connective Profile Kosin Medical Journal anti-extractable nuclear antigen antibodies antinuclear antibodies autoimmunity |
| title | Agreement of three commercial anti-extractable nuclear antigen tests: EUROASSAY Anti-ENA Profile, Polycheck Autoimmune Test and FID Connective Profile |
| title_full | Agreement of three commercial anti-extractable nuclear antigen tests: EUROASSAY Anti-ENA Profile, Polycheck Autoimmune Test and FID Connective Profile |
| title_fullStr | Agreement of three commercial anti-extractable nuclear antigen tests: EUROASSAY Anti-ENA Profile, Polycheck Autoimmune Test and FID Connective Profile |
| title_full_unstemmed | Agreement of three commercial anti-extractable nuclear antigen tests: EUROASSAY Anti-ENA Profile, Polycheck Autoimmune Test and FID Connective Profile |
| title_short | Agreement of three commercial anti-extractable nuclear antigen tests: EUROASSAY Anti-ENA Profile, Polycheck Autoimmune Test and FID Connective Profile |
| title_sort | agreement of three commercial anti extractable nuclear antigen tests euroassay anti ena profile polycheck autoimmune test and fid connective profile |
| topic | anti-extractable nuclear antigen antibodies antinuclear antibodies autoimmunity |
| url | http://www.kosinmedj.org/upload/pdf/ksmc033-03-04.pdf |
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