Ameliorative effect of apelin-13 against renal complications in L-NAME-induced preeclampsia in rats
Pre-eclampsia (PE) accompanying acute liver and kidney injury has remained a master cause of both fetal and maternal mortality and morbidity. Vasoactive mediators, oxidative stress and inflammatory imbalanceshave an important role in PE pathogenesis. Apelin is an adipokine that improves endothelial...
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PeerJ Inc.
2021-03-01
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| author | Reham Z. Hamza Abdel Aziz A. Diab Mansour H. Zahra Ali K. Asalah Mai S. Attia Suzan MM Moursi |
| author_facet | Reham Z. Hamza Abdel Aziz A. Diab Mansour H. Zahra Ali K. Asalah Mai S. Attia Suzan MM Moursi |
| author_sort | Reham Z. Hamza |
| collection | DOAJ |
| description | Pre-eclampsia (PE) accompanying acute liver and kidney injury has remained a master cause of both fetal and maternal mortality and morbidity. Vasoactive mediators, oxidative stress and inflammatory imbalanceshave an important role in PE pathogenesis. Apelin is an adipokine that improves endothelial dysfunction; has anti-inflammatory and antioxidant effects; moreover, its level reduced during PE. This study aimed to explore the effects of apelin-13 administration on preeclampsia-associated renal dysfunction and proteinuria. Thirty-three pregnant female rats were divided into three groups; group: 1 (normal pregnant rats), group: 2 (preeclamptic rats); where rats were injected subcutaneously with 75 mg L-NAME/ kg body weight/day beginning from 9th to 20th day of pregnancy andgroup 3 (apelin-13 treated preeclamptic rats); In which L-NAME-induced preeclamptic rats were subcutaneously injected with 6 × 10−8 mol apelin-13/kg body weight/twice daily starting from 6th to 20th day of pregnancy. In all groups, mean arterial blood pressure, total urine protein, serum urea, creatinine, nitric oxide (NO), endothelin-1 (ET-1), interleukin–6 (IL-6) and malondialdhyde (MDA) were measured. Histopathological examination of kidney tissues was also done. preeclamptic rats showed significantly increased mean arterial blood pressure, total urine proteins, serum urea, creatinine, ET-1, IL-6, and MDA, but revealed a significantly decreased serum NO level. On the other hand, apelin treatment significantly improved these parameters together with amelioration of kidney histoarchitecture in the treated group. In conclusion, apelin may be a potentially curative candidate for prohibiting kidney damage and have a therapeutic benefit in PE rat models. |
| first_indexed | 2024-03-09T08:20:38Z |
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| last_indexed | 2024-03-09T08:20:38Z |
| publishDate | 2021-03-01 |
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| spelling | doaj.art-58f545d596064ac091210dfbbc2f366e2023-12-02T21:49:53ZengPeerJ Inc.PeerJ2167-83592021-03-019e1111010.7717/peerj.11110Ameliorative effect of apelin-13 against renal complications in L-NAME-induced preeclampsia in ratsReham Z. Hamza0Abdel Aziz A. Diab1Mansour H. Zahra2Ali K. Asalah3Mai S. Attia4Suzan MM Moursi5Biology Department, College of Sciences, Taif University, Taif, Taif, Saudi ArabiaZoology Department, Faculty of Science, Zagazig University, Zagazig, EgyptZoology Department, Faculty of Science, Zagazig University, Zagazig, EgyptMedical Physiology Department - Faculty of Human Medicine, Zagazig University, Zagazig, EgyptZoology Department, Faculty of Science, Zagazig University, Zagazig, EgyptMedical Physiology Department - Faculty of Human Medicine, Zagazig University, Zagazig, EgyptPre-eclampsia (PE) accompanying acute liver and kidney injury has remained a master cause of both fetal and maternal mortality and morbidity. Vasoactive mediators, oxidative stress and inflammatory imbalanceshave an important role in PE pathogenesis. Apelin is an adipokine that improves endothelial dysfunction; has anti-inflammatory and antioxidant effects; moreover, its level reduced during PE. This study aimed to explore the effects of apelin-13 administration on preeclampsia-associated renal dysfunction and proteinuria. Thirty-three pregnant female rats were divided into three groups; group: 1 (normal pregnant rats), group: 2 (preeclamptic rats); where rats were injected subcutaneously with 75 mg L-NAME/ kg body weight/day beginning from 9th to 20th day of pregnancy andgroup 3 (apelin-13 treated preeclamptic rats); In which L-NAME-induced preeclamptic rats were subcutaneously injected with 6 × 10−8 mol apelin-13/kg body weight/twice daily starting from 6th to 20th day of pregnancy. In all groups, mean arterial blood pressure, total urine protein, serum urea, creatinine, nitric oxide (NO), endothelin-1 (ET-1), interleukin–6 (IL-6) and malondialdhyde (MDA) were measured. Histopathological examination of kidney tissues was also done. preeclamptic rats showed significantly increased mean arterial blood pressure, total urine proteins, serum urea, creatinine, ET-1, IL-6, and MDA, but revealed a significantly decreased serum NO level. On the other hand, apelin treatment significantly improved these parameters together with amelioration of kidney histoarchitecture in the treated group. In conclusion, apelin may be a potentially curative candidate for prohibiting kidney damage and have a therapeutic benefit in PE rat models.https://peerj.com/articles/11110.pdfApelinPreeclampsiaProteinuriaKidney injury |
| spellingShingle | Reham Z. Hamza Abdel Aziz A. Diab Mansour H. Zahra Ali K. Asalah Mai S. Attia Suzan MM Moursi Ameliorative effect of apelin-13 against renal complications in L-NAME-induced preeclampsia in rats PeerJ Apelin Preeclampsia Proteinuria Kidney injury |
| title | Ameliorative effect of apelin-13 against renal complications in L-NAME-induced preeclampsia in rats |
| title_full | Ameliorative effect of apelin-13 against renal complications in L-NAME-induced preeclampsia in rats |
| title_fullStr | Ameliorative effect of apelin-13 against renal complications in L-NAME-induced preeclampsia in rats |
| title_full_unstemmed | Ameliorative effect of apelin-13 against renal complications in L-NAME-induced preeclampsia in rats |
| title_short | Ameliorative effect of apelin-13 against renal complications in L-NAME-induced preeclampsia in rats |
| title_sort | ameliorative effect of apelin 13 against renal complications in l name induced preeclampsia in rats |
| topic | Apelin Preeclampsia Proteinuria Kidney injury |
| url | https://peerj.com/articles/11110.pdf |
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