Protective Effect of Spore Powder of <i>Antrodia camphorata</i> ATCC 200183 on CCl<sub>4</sub>-Induced Liver Fibrosis in Mice
Liver fibrosis is a pathological process with intrahepatic diffused deposition of the excess extracellular matrix, which leads to various chronic liver diseases. Drugs with high efficacy and low toxicity for liver fibrosis are still unavailable. <i>Antrodia camphorata</i> has antioxidant...
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MDPI AG
2020-09-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/9/2778 |
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| author | Yilin Ren Hua-Xiang Li Lingxi Zhou Zhen-Ming Lu Jinsong Shi Yan Geng Zheng-Hong Xu |
| author_facet | Yilin Ren Hua-Xiang Li Lingxi Zhou Zhen-Ming Lu Jinsong Shi Yan Geng Zheng-Hong Xu |
| author_sort | Yilin Ren |
| collection | DOAJ |
| description | Liver fibrosis is a pathological process with intrahepatic diffused deposition of the excess extracellular matrix, which leads to various chronic liver diseases. Drugs with high efficacy and low toxicity for liver fibrosis are still unavailable. <i>Antrodia camphorata</i> has antioxidant, antivirus, antitumor and anti-inflammation roles, and has been used to treat liver diseases in the population. However, the hepatoprotective effects of <i>A. camphorata</i> spores and the mechanisms behind it have not been investigated. In this study, we evaluate the hepatoprotective effect of spore powder of <i>A. camphorata</i> (SP, 100 mg/kg/day or 200 mg/kg/day) on carbon tetrachloride (CCl<sub>4</sub>)-induced liver fibrosis in mice. SP groups reduced serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities compared with the CCl<sub>4</sub> group. SP also showed a decrease in hydroxyproline (Hyp) content in liver tissues. SP improved cell damage and reduced collagen deposition by H&E, Sirius red and Masson staining. Furthermore, SP down-regulated the mRNA levels of <i>α-SMA</i> and <i>Col 1</i>, and the protein expression of α-smooth muscle actin (α-SMA), collagen I (Col 1), tumor necrosis factor alpha (TNF-α), toll like receptor 4 (TLR4) and nuclear factor-Κb (NF-κB) p65. In summary, SP has an ameliorative effect on hepatic fibrosis, probably by inhibiting the activation of hepatic stellate cells, reducing the synthesis of extracellular matrix. |
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| last_indexed | 2024-03-10T16:24:23Z |
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| spelling | doaj.art-58f6b6348e974f0383d34128ab585e2b2023-11-20T13:24:25ZengMDPI AGNutrients2072-66432020-09-01129277810.3390/nu12092778Protective Effect of Spore Powder of <i>Antrodia camphorata</i> ATCC 200183 on CCl<sub>4</sub>-Induced Liver Fibrosis in MiceYilin Ren0Hua-Xiang Li1Lingxi Zhou2Zhen-Ming Lu3Jinsong Shi4Yan Geng5Zheng-Hong Xu6School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225127, ChinaKey Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, ChinaNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, ChinaSchool of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, ChinaNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, ChinaLiver fibrosis is a pathological process with intrahepatic diffused deposition of the excess extracellular matrix, which leads to various chronic liver diseases. Drugs with high efficacy and low toxicity for liver fibrosis are still unavailable. <i>Antrodia camphorata</i> has antioxidant, antivirus, antitumor and anti-inflammation roles, and has been used to treat liver diseases in the population. However, the hepatoprotective effects of <i>A. camphorata</i> spores and the mechanisms behind it have not been investigated. In this study, we evaluate the hepatoprotective effect of spore powder of <i>A. camphorata</i> (SP, 100 mg/kg/day or 200 mg/kg/day) on carbon tetrachloride (CCl<sub>4</sub>)-induced liver fibrosis in mice. SP groups reduced serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities compared with the CCl<sub>4</sub> group. SP also showed a decrease in hydroxyproline (Hyp) content in liver tissues. SP improved cell damage and reduced collagen deposition by H&E, Sirius red and Masson staining. Furthermore, SP down-regulated the mRNA levels of <i>α-SMA</i> and <i>Col 1</i>, and the protein expression of α-smooth muscle actin (α-SMA), collagen I (Col 1), tumor necrosis factor alpha (TNF-α), toll like receptor 4 (TLR4) and nuclear factor-Κb (NF-κB) p65. In summary, SP has an ameliorative effect on hepatic fibrosis, probably by inhibiting the activation of hepatic stellate cells, reducing the synthesis of extracellular matrix.https://www.mdpi.com/2072-6643/12/9/2778<i>Antrodia camphorata</i> sporeliver damagehepatic stellate cellsimmunity |
| spellingShingle | Yilin Ren Hua-Xiang Li Lingxi Zhou Zhen-Ming Lu Jinsong Shi Yan Geng Zheng-Hong Xu Protective Effect of Spore Powder of <i>Antrodia camphorata</i> ATCC 200183 on CCl<sub>4</sub>-Induced Liver Fibrosis in Mice Nutrients <i>Antrodia camphorata</i> spore liver damage hepatic stellate cells immunity |
| title | Protective Effect of Spore Powder of <i>Antrodia camphorata</i> ATCC 200183 on CCl<sub>4</sub>-Induced Liver Fibrosis in Mice |
| title_full | Protective Effect of Spore Powder of <i>Antrodia camphorata</i> ATCC 200183 on CCl<sub>4</sub>-Induced Liver Fibrosis in Mice |
| title_fullStr | Protective Effect of Spore Powder of <i>Antrodia camphorata</i> ATCC 200183 on CCl<sub>4</sub>-Induced Liver Fibrosis in Mice |
| title_full_unstemmed | Protective Effect of Spore Powder of <i>Antrodia camphorata</i> ATCC 200183 on CCl<sub>4</sub>-Induced Liver Fibrosis in Mice |
| title_short | Protective Effect of Spore Powder of <i>Antrodia camphorata</i> ATCC 200183 on CCl<sub>4</sub>-Induced Liver Fibrosis in Mice |
| title_sort | protective effect of spore powder of i antrodia camphorata i atcc 200183 on ccl sub 4 sub induced liver fibrosis in mice |
| topic | <i>Antrodia camphorata</i> spore liver damage hepatic stellate cells immunity |
| url | https://www.mdpi.com/2072-6643/12/9/2778 |
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