Use of <it>in vivo</it>-induced antigen technology (IVIAT) for the identification of <it>Streptococcus suis </it>serotype 2 <it>in vivo</it>-induced bacterial protein antigens
<p>Abstract</p> <p>Background</p> <p><it>Streptococcus suis </it>serotype 2 (SS2) is a zoonotic agent that causes death and disease in both humans and swine. A better understanding of SS2-host molecular interactions is crucial for understanding SS2 pathogene...
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| Format: | Article |
| Language: | English |
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BMC
2009-09-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/9/201 |
| _version_ | 1818755627936120832 |
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| author | Lu Chengping Zhu Haodan Gu Hongwei |
| author_facet | Lu Chengping Zhu Haodan Gu Hongwei |
| author_sort | Lu Chengping |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Streptococcus suis </it>serotype 2 (SS2) is a zoonotic agent that causes death and disease in both humans and swine. A better understanding of SS2-host molecular interactions is crucial for understanding SS2 pathogenesis and immunology. Conventional genetic and biochemical approaches used to study SS2 virulence factors are unable to take into account the complex and dynamic environmental stimuli associated with the infection process. In this study, <it>in vivo</it>-induced antigen technology (IVIAT), an immunoscreening technique, was used to identify the immunogenic bacterial proteins that are induced or upregulated <it>in vivo </it>during SS2 infection.</p> <p>Results</p> <p>Convalescent-phase sera from pigs infected with SS2 were pooled, adsorbed against <it>in vitro </it>antigens, and used to screen SS2 genomic expression libraries. Upon analysis of the identified proteins, we were able to assign a putative function to 40 of the 48 proteins. These included proteins implicated in cell envelope structure, regulation, molecule synthesis, substance and energy metabolism, transport, translation, and those with unknown functions. The <it>in vivo</it>-induced changes in the expression of 10 of these 40 genes were measured using real-time reverse transcription (RT)-PCR, revealing that the expression of 6 of the 10 genes was upregulated in the <it>in vivo </it>condition. The strain distribution of these 10 genes was analyzed by PCR, and they were found in the most virulent SS2 strains. In addition, protein sequence alignments of the newly identified proteins demonstrate that three are putative virulence-associated proteins.</p> <p>Conclusion</p> <p>Collectively, our results suggest that these <it>in vivo</it>-induced or upregulated genes may contribute to SS2 disease development. We hypothesize that the identification of factors specifically induced or upregulated during SS2 infection will aid in our understanding of SS2 pathogenesis and may contribute to the control SS2 outbreaks. In addition, the proteins identified using IVIAT may be useful potential vaccine candidates or virulence markers.</p> |
| first_indexed | 2024-12-18T05:42:10Z |
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| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-18T05:42:10Z |
| publishDate | 2009-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-58fcf49c99004e1aa1203d6d32bd62622022-12-21T21:19:09ZengBMCBMC Microbiology1471-21802009-09-019120110.1186/1471-2180-9-201Use of <it>in vivo</it>-induced antigen technology (IVIAT) for the identification of <it>Streptococcus suis </it>serotype 2 <it>in vivo</it>-induced bacterial protein antigensLu ChengpingZhu HaodanGu Hongwei<p>Abstract</p> <p>Background</p> <p><it>Streptococcus suis </it>serotype 2 (SS2) is a zoonotic agent that causes death and disease in both humans and swine. A better understanding of SS2-host molecular interactions is crucial for understanding SS2 pathogenesis and immunology. Conventional genetic and biochemical approaches used to study SS2 virulence factors are unable to take into account the complex and dynamic environmental stimuli associated with the infection process. In this study, <it>in vivo</it>-induced antigen technology (IVIAT), an immunoscreening technique, was used to identify the immunogenic bacterial proteins that are induced or upregulated <it>in vivo </it>during SS2 infection.</p> <p>Results</p> <p>Convalescent-phase sera from pigs infected with SS2 were pooled, adsorbed against <it>in vitro </it>antigens, and used to screen SS2 genomic expression libraries. Upon analysis of the identified proteins, we were able to assign a putative function to 40 of the 48 proteins. These included proteins implicated in cell envelope structure, regulation, molecule synthesis, substance and energy metabolism, transport, translation, and those with unknown functions. The <it>in vivo</it>-induced changes in the expression of 10 of these 40 genes were measured using real-time reverse transcription (RT)-PCR, revealing that the expression of 6 of the 10 genes was upregulated in the <it>in vivo </it>condition. The strain distribution of these 10 genes was analyzed by PCR, and they were found in the most virulent SS2 strains. In addition, protein sequence alignments of the newly identified proteins demonstrate that three are putative virulence-associated proteins.</p> <p>Conclusion</p> <p>Collectively, our results suggest that these <it>in vivo</it>-induced or upregulated genes may contribute to SS2 disease development. We hypothesize that the identification of factors specifically induced or upregulated during SS2 infection will aid in our understanding of SS2 pathogenesis and may contribute to the control SS2 outbreaks. In addition, the proteins identified using IVIAT may be useful potential vaccine candidates or virulence markers.</p>http://www.biomedcentral.com/1471-2180/9/201 |
| spellingShingle | Lu Chengping Zhu Haodan Gu Hongwei Use of <it>in vivo</it>-induced antigen technology (IVIAT) for the identification of <it>Streptococcus suis </it>serotype 2 <it>in vivo</it>-induced bacterial protein antigens BMC Microbiology |
| title | Use of <it>in vivo</it>-induced antigen technology (IVIAT) for the identification of <it>Streptococcus suis </it>serotype 2 <it>in vivo</it>-induced bacterial protein antigens |
| title_full | Use of <it>in vivo</it>-induced antigen technology (IVIAT) for the identification of <it>Streptococcus suis </it>serotype 2 <it>in vivo</it>-induced bacterial protein antigens |
| title_fullStr | Use of <it>in vivo</it>-induced antigen technology (IVIAT) for the identification of <it>Streptococcus suis </it>serotype 2 <it>in vivo</it>-induced bacterial protein antigens |
| title_full_unstemmed | Use of <it>in vivo</it>-induced antigen technology (IVIAT) for the identification of <it>Streptococcus suis </it>serotype 2 <it>in vivo</it>-induced bacterial protein antigens |
| title_short | Use of <it>in vivo</it>-induced antigen technology (IVIAT) for the identification of <it>Streptococcus suis </it>serotype 2 <it>in vivo</it>-induced bacterial protein antigens |
| title_sort | use of it in vivo it induced antigen technology iviat for the identification of it streptococcus suis it serotype 2 it in vivo it induced bacterial protein antigens |
| url | http://www.biomedcentral.com/1471-2180/9/201 |
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