Root Conditioning with Citric Acid and Ethylenediaminetetraacetic Acid and their Effect on Fibrin Clot Adhesion to Dentin-A Scanning Electron Microscopic Study
Introduction: Root biomodifiers are used to remove the smear layer formed on the roots as a result of mechanical debridement and also to expose collagen fibers making the root surfaces biologically acceptable. Further, the blood element linkage to demineralized roots and clot stabilization by co...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-08-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/10443/27768_CE(RA1)_F(GG)PF1_(SY_PY)_PFA(SY_SS).pdf |
| Summary: | Introduction: Root biomodifiers are used to remove the smear
layer formed on the roots as a result of mechanical debridement
and also to expose collagen fibers making the root surfaces
biologically acceptable. Further, the blood element linkage to
demineralized roots and clot stabilization by collagen fibres are
vital for the wound healing and success of periodontal surgery.
Aim: The aim of this study was to evaluate and compare the
distribution of fibrin network to dentin surfaces conditioned with
citric acid and Ethylenediaminetetraacetic Acid (EDTA) using
Scanning Electron Microscope (SEM).
Materials and Methods: Thirty human dentin blocks (4 mm x
3 mm x 1 mm) were prepared and equally divided into three
groups. The control Group A specimens were treated with
Phosphate Buffered Saline (PBS). The study Group B and C
specimens were treated with citric Acid and EDTA respectively.
After conditioning, a drop of fresh human whole blood was
placed on the dentin specimens and was allowed to clot. The
specimens were then processed using standardized protocol for
SEM. The dentin surfaces were then analysed for fibrin network
linkage under SEM and scored according to the degree of fibrin
linkage to the dentin surface. Statistical analysis was done
using Kruskal Wallis test to compare the score values between
the study groups. Pairwise comparison between the study
groups was done using Mann Whitney U test with Bonferroni
correction.
Results: The fibrin network formed on the dentin network
were analysed and scores were given. Kruskal Wallis test and
Mann Whitney U test with Bonferroni correction were used
for statistical analysis. It was found that most of the dentin
specimens conditioned with citric acid and covered with
human blood exhibited dense fibrin network compared to
EDTA. However, statistical analysis showed that there were no
significant differences found between the groups (p=0.35).
Conclusion: Citric acid and EDTA has almost comparable
effect in promoting the adhesion of fibrin network onto the
dentin surface which may further enhance connective tissue
attachment to the root surface. |
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| ISSN: | 2249-782X 0973-709X |