Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma
Summary: This protocol provides the steps required for the establishment of patient-derived xenograft (PDX) tumors for head and neck squamous cell carcinomas (HNSCCs) and their utility in examining drug responses. PDXs recapitulate the heterogeneity observed in the corresponding human tumors, which...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | STAR Protocols |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720300113 |
| _version_ | 1818245024704364544 |
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| author | Christina Karamboulas Jalna Meens Laurie Ailles |
| author_facet | Christina Karamboulas Jalna Meens Laurie Ailles |
| author_sort | Christina Karamboulas |
| collection | DOAJ |
| description | Summary: This protocol provides the steps required for the establishment of patient-derived xenograft (PDX) tumors for head and neck squamous cell carcinomas (HNSCCs) and their utility in examining drug responses. PDXs recapitulate the heterogeneity observed in the corresponding human tumors, which makes them an ideal pre-clinical model system. This protocol outlines the detailed steps required for (1) the generation of HNSCC-PDXs, (2) the processing of tumor tissues, and (3) the expansion of PDX models into cohorts for (4) drug testing.For complete details on the use and execution of this protocol please refer to Karamboulas et al. (2018). |
| first_indexed | 2024-12-12T14:26:20Z |
| format | Article |
| id | doaj.art-59043720aec6428fadeb9e2e11a2bb49 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-12T14:26:20Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-59043720aec6428fadeb9e2e11a2bb492022-12-22T00:21:40ZengElsevierSTAR Protocols2666-16672020-06-0111100024Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell CarcinomaChristina Karamboulas0Jalna Meens1Laurie Ailles2Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1L7, Canada; Corresponding authorPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1L7, CanadaPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 1L7, Canada; Department of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Corresponding authorSummary: This protocol provides the steps required for the establishment of patient-derived xenograft (PDX) tumors for head and neck squamous cell carcinomas (HNSCCs) and their utility in examining drug responses. PDXs recapitulate the heterogeneity observed in the corresponding human tumors, which makes them an ideal pre-clinical model system. This protocol outlines the detailed steps required for (1) the generation of HNSCC-PDXs, (2) the processing of tumor tissues, and (3) the expansion of PDX models into cohorts for (4) drug testing.For complete details on the use and execution of this protocol please refer to Karamboulas et al. (2018).http://www.sciencedirect.com/science/article/pii/S2666166720300113 |
| spellingShingle | Christina Karamboulas Jalna Meens Laurie Ailles Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma STAR Protocols |
| title | Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma |
| title_full | Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma |
| title_fullStr | Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma |
| title_full_unstemmed | Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma |
| title_short | Establishment and Use of Patient-Derived Xenograft Models for Drug Testing in Head and Neck Squamous Cell Carcinoma |
| title_sort | establishment and use of patient derived xenograft models for drug testing in head and neck squamous cell carcinoma |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720300113 |
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