Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats
Satellite DNAs are tandemly repeated sequences preferentially assembled into large arrays within constitutive heterochromatin and their transcription is often activated by stress conditions, particularly by heat stress. Bioinformatic analyses of sequenced genomes however reveal single repeats or sho...
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MDPI AG
2020-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/6/663 |
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| author | Isidoro Feliciello Antonio Sermek Željka Pezer Maja Matulić Đurđica Ugarković |
| author_facet | Isidoro Feliciello Antonio Sermek Željka Pezer Maja Matulić Đurđica Ugarković |
| author_sort | Isidoro Feliciello |
| collection | DOAJ |
| description | Satellite DNAs are tandemly repeated sequences preferentially assembled into large arrays within constitutive heterochromatin and their transcription is often activated by stress conditions, particularly by heat stress. Bioinformatic analyses of sequenced genomes however reveal single repeats or short arrays of satellite DNAs dispersed in the vicinity of genes within euchromatin. Here, we analyze transcription of a major human alpha satellite DNA upon heat stress and follow the dynamics of “silent” H3K9me3 and “active” H3K4me2/3 histone marks at dispersed euchromatic and tandemly arranged heterochromatic alpha repeats. The results show H3K9me3 enrichment at alpha repeats upon heat stress, which correlates with the dynamics of alpha satellite DNA transcription activation, while no change in H3K4me2/3 level is detected. Spreading of H3K9me3 up to 1–2 kb from the insertion sites of the euchromatic alpha repeats is detected, revealing the alpha repeats as modulators of local chromatin structure. In addition, expression of genes containing alpha repeats within introns as well as of genes closest to the intergenic alpha repeats is downregulated upon heat stress. Further studies are necessary to reveal the possible contribution of H3K9me3 enriched alpha repeats, in particular those located within introns, to the silencing of their associated genes. |
| first_indexed | 2024-03-10T19:04:07Z |
| format | Article |
| id | doaj.art-5909f0e499f64c728da63c351d26ac06 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T19:04:07Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-5909f0e499f64c728da63c351d26ac062023-11-20T04:14:34ZengMDPI AGGenes2073-44252020-06-0111666310.3390/genes11060663Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA RepeatsIsidoro Feliciello0Antonio Sermek1Željka Pezer2Maja Matulić3Đurđica Ugarković4Department of Molecular Biology, Ruder Bošković Institute, Bijenička 54, HR-10000 Zagreb, CroatiaDepartment of Molecular Biology, Ruder Bošković Institute, Bijenička 54, HR-10000 Zagreb, CroatiaDepartment of Molecular Biology, Ruder Bošković Institute, Bijenička 54, HR-10000 Zagreb, CroatiaDepartment of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, CroatiaDepartment of Molecular Biology, Ruder Bošković Institute, Bijenička 54, HR-10000 Zagreb, CroatiaSatellite DNAs are tandemly repeated sequences preferentially assembled into large arrays within constitutive heterochromatin and their transcription is often activated by stress conditions, particularly by heat stress. Bioinformatic analyses of sequenced genomes however reveal single repeats or short arrays of satellite DNAs dispersed in the vicinity of genes within euchromatin. Here, we analyze transcription of a major human alpha satellite DNA upon heat stress and follow the dynamics of “silent” H3K9me3 and “active” H3K4me2/3 histone marks at dispersed euchromatic and tandemly arranged heterochromatic alpha repeats. The results show H3K9me3 enrichment at alpha repeats upon heat stress, which correlates with the dynamics of alpha satellite DNA transcription activation, while no change in H3K4me2/3 level is detected. Spreading of H3K9me3 up to 1–2 kb from the insertion sites of the euchromatic alpha repeats is detected, revealing the alpha repeats as modulators of local chromatin structure. In addition, expression of genes containing alpha repeats within introns as well as of genes closest to the intergenic alpha repeats is downregulated upon heat stress. Further studies are necessary to reveal the possible contribution of H3K9me3 enriched alpha repeats, in particular those located within introns, to the silencing of their associated genes.https://www.mdpi.com/2073-4425/11/6/663alpha satellite DNAheterochromatineuchromatintranscriptionheat stresshistone modification |
| spellingShingle | Isidoro Feliciello Antonio Sermek Željka Pezer Maja Matulić Đurđica Ugarković Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats Genes alpha satellite DNA heterochromatin euchromatin transcription heat stress histone modification |
| title | Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats |
| title_full | Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats |
| title_fullStr | Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats |
| title_full_unstemmed | Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats |
| title_short | Heat Stress Affects H3K9me3 Level at Human Alpha Satellite DNA Repeats |
| title_sort | heat stress affects h3k9me3 level at human alpha satellite dna repeats |
| topic | alpha satellite DNA heterochromatin euchromatin transcription heat stress histone modification |
| url | https://www.mdpi.com/2073-4425/11/6/663 |
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