Seamless gene tagging by endonuclease-driven homologous recombination.
Gene tagging facilitates systematic genomic and proteomic analyses but chromosomal tagging typically disrupts gene regulatory sequences. Here we describe a seamless gene tagging approach that preserves endogenous gene regulation and is potentially applicable in any species with efficient DNA double-...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3161820?pdf=render |
| _version_ | 1818010861494599680 |
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| author | Anton Khmelinskii Matthias Meurer Nurlanbek Duishoev Nicolas Delhomme Michael Knop |
| author_facet | Anton Khmelinskii Matthias Meurer Nurlanbek Duishoev Nicolas Delhomme Michael Knop |
| author_sort | Anton Khmelinskii |
| collection | DOAJ |
| description | Gene tagging facilitates systematic genomic and proteomic analyses but chromosomal tagging typically disrupts gene regulatory sequences. Here we describe a seamless gene tagging approach that preserves endogenous gene regulation and is potentially applicable in any species with efficient DNA double-strand break repair by homologous recombination. We implement seamless tagging in Saccharomyces cerevisiae and demonstrate its application for protein tagging while preserving simultaneously upstream and downstream gene regulatory elements. Seamless tagging is compatible with high-throughput strain construction using synthetic genetic arrays (SGA), enables functional analysis of transcription antisense to open reading frames and should facilitate systematic and minimally-invasive analysis of gene functions. |
| first_indexed | 2024-04-14T06:00:49Z |
| format | Article |
| id | doaj.art-590beabfb67f423ab195a666516835f7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T06:00:49Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-590beabfb67f423ab195a666516835f72022-12-22T02:08:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2379410.1371/journal.pone.0023794Seamless gene tagging by endonuclease-driven homologous recombination.Anton KhmelinskiiMatthias MeurerNurlanbek DuishoevNicolas DelhommeMichael KnopGene tagging facilitates systematic genomic and proteomic analyses but chromosomal tagging typically disrupts gene regulatory sequences. Here we describe a seamless gene tagging approach that preserves endogenous gene regulation and is potentially applicable in any species with efficient DNA double-strand break repair by homologous recombination. We implement seamless tagging in Saccharomyces cerevisiae and demonstrate its application for protein tagging while preserving simultaneously upstream and downstream gene regulatory elements. Seamless tagging is compatible with high-throughput strain construction using synthetic genetic arrays (SGA), enables functional analysis of transcription antisense to open reading frames and should facilitate systematic and minimally-invasive analysis of gene functions.http://europepmc.org/articles/PMC3161820?pdf=render |
| spellingShingle | Anton Khmelinskii Matthias Meurer Nurlanbek Duishoev Nicolas Delhomme Michael Knop Seamless gene tagging by endonuclease-driven homologous recombination. PLoS ONE |
| title | Seamless gene tagging by endonuclease-driven homologous recombination. |
| title_full | Seamless gene tagging by endonuclease-driven homologous recombination. |
| title_fullStr | Seamless gene tagging by endonuclease-driven homologous recombination. |
| title_full_unstemmed | Seamless gene tagging by endonuclease-driven homologous recombination. |
| title_short | Seamless gene tagging by endonuclease-driven homologous recombination. |
| title_sort | seamless gene tagging by endonuclease driven homologous recombination |
| url | http://europepmc.org/articles/PMC3161820?pdf=render |
| work_keys_str_mv | AT antonkhmelinskii seamlessgenetaggingbyendonucleasedrivenhomologousrecombination AT matthiasmeurer seamlessgenetaggingbyendonucleasedrivenhomologousrecombination AT nurlanbekduishoev seamlessgenetaggingbyendonucleasedrivenhomologousrecombination AT nicolasdelhomme seamlessgenetaggingbyendonucleasedrivenhomologousrecombination AT michaelknop seamlessgenetaggingbyendonucleasedrivenhomologousrecombination |