Analysis of lysine acetylation in tomato spot wilt virus infection in Nicotiana benthamiana
IntroductionKac is a model for all acylation modification studies. Kac plays a critical role in eukaryotes and prokaryotes. It is mainly involved in six major biological functions: gene expression, signal transduction, cell development, protein conversion, metabolism, and metabolite transport.Method...
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| Format: | Article |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1046163/full |
| _version_ | 1797934748727771136 |
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| author | Yanwei Gong Ying Li Dongyang Liu Lianqiang Jiang Hui Liang Yuanhua Wu Fenglong Wang Jinguang Yang |
| author_facet | Yanwei Gong Ying Li Dongyang Liu Lianqiang Jiang Hui Liang Yuanhua Wu Fenglong Wang Jinguang Yang |
| author_sort | Yanwei Gong |
| collection | DOAJ |
| description | IntroductionKac is a model for all acylation modification studies. Kac plays a critical role in eukaryotes and prokaryotes. It is mainly involved in six major biological functions: gene expression, signal transduction, cell development, protein conversion, metabolism, and metabolite transport.MethodWe investigated and compared the acetylation modification of proteins in healthy and tomato spot wilt virus (TSWV)-infected Nicotiana benthamiana leaves.ResultWe identified 3,418 acetylated lysine sites on 1962 proteins acetylation of proteins in the TSWV-infected and control groups were compared; it was observed that 408 sites on 294 proteins were upregulated and 284 sites on 219 proteins (involved in pentose phosphate, photosynthesis, and carbon fixation in photosynthesis) were downregulated after the infection. Overall, 35 conserved motifs were identified, of which xxxkxxxxx_K_ Rxxxxxxxxx represented 1,334 (31.63%) enrichment motifs and was the most common combination. Bioinformatic analysis revealed that most of the proteins with Kac sites were located in the chloroplast and cytoplasm. They were involved in biological processes, such as cellular and metabolic processes.DiscussionIn conclusion, our results revealed that Kac may participate in the regulation of TSWV infection in N. benthamiana. |
| first_indexed | 2024-04-10T18:03:43Z |
| format | Article |
| id | doaj.art-590c021dabde497582df9a442abeff49 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T18:03:43Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-590c021dabde497582df9a442abeff492023-02-02T13:43:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-02-011410.3389/fmicb.2023.10461631046163Analysis of lysine acetylation in tomato spot wilt virus infection in Nicotiana benthamianaYanwei Gong0Ying Li1Dongyang Liu2Lianqiang Jiang3Hui Liang4Yuanhua Wu5Fenglong Wang6Jinguang Yang7College of Plant Protection, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Tobacco Pest Monitoring, Controlling and Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaLiangshan State Company of Sichuan Province Tobacco Company, Mile, ChinaLiangshan State Company of Sichuan Province Tobacco Company, Mile, ChinaLiangshan State Company of Sichuan Province Tobacco Company, Mile, ChinaCollege of Plant Protection, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Tobacco Pest Monitoring, Controlling and Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaKey Laboratory of Tobacco Pest Monitoring, Controlling and Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, ChinaIntroductionKac is a model for all acylation modification studies. Kac plays a critical role in eukaryotes and prokaryotes. It is mainly involved in six major biological functions: gene expression, signal transduction, cell development, protein conversion, metabolism, and metabolite transport.MethodWe investigated and compared the acetylation modification of proteins in healthy and tomato spot wilt virus (TSWV)-infected Nicotiana benthamiana leaves.ResultWe identified 3,418 acetylated lysine sites on 1962 proteins acetylation of proteins in the TSWV-infected and control groups were compared; it was observed that 408 sites on 294 proteins were upregulated and 284 sites on 219 proteins (involved in pentose phosphate, photosynthesis, and carbon fixation in photosynthesis) were downregulated after the infection. Overall, 35 conserved motifs were identified, of which xxxkxxxxx_K_ Rxxxxxxxxx represented 1,334 (31.63%) enrichment motifs and was the most common combination. Bioinformatic analysis revealed that most of the proteins with Kac sites were located in the chloroplast and cytoplasm. They were involved in biological processes, such as cellular and metabolic processes.DiscussionIn conclusion, our results revealed that Kac may participate in the regulation of TSWV infection in N. benthamiana.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1046163/fulllysine acetylationTSWVprotein modificationsNicotiana benthamianaRBCLpost-translational |
| spellingShingle | Yanwei Gong Ying Li Dongyang Liu Lianqiang Jiang Hui Liang Yuanhua Wu Fenglong Wang Jinguang Yang Analysis of lysine acetylation in tomato spot wilt virus infection in Nicotiana benthamiana Frontiers in Microbiology lysine acetylation TSWV protein modifications Nicotiana benthamiana RBCL post-translational |
| title | Analysis of lysine acetylation in tomato spot wilt virus infection in Nicotiana benthamiana |
| title_full | Analysis of lysine acetylation in tomato spot wilt virus infection in Nicotiana benthamiana |
| title_fullStr | Analysis of lysine acetylation in tomato spot wilt virus infection in Nicotiana benthamiana |
| title_full_unstemmed | Analysis of lysine acetylation in tomato spot wilt virus infection in Nicotiana benthamiana |
| title_short | Analysis of lysine acetylation in tomato spot wilt virus infection in Nicotiana benthamiana |
| title_sort | analysis of lysine acetylation in tomato spot wilt virus infection in nicotiana benthamiana |
| topic | lysine acetylation TSWV protein modifications Nicotiana benthamiana RBCL post-translational |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1046163/full |
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