A decrease of docosahexaenoic acid in testes of mice fed a high-fat diet is associated with impaired sperm acrosome reaction and fertility
Obesity is a major worldwide health problem that is related to most chronic diseases, including male infertility. Owing to its wide impact on health, mechanisms underlying obesity-related infertility remain unknown. In this study, we report that mice fed a high-fat diet (HFD) for over 2 months showe...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Asian Journal of Andrology |
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| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2021;volume=23;issue=3;spage=306;epage=313;aulast=Bunay |
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| author | Julio Bunay Luz-Maria Gallardo Jorge Luis Torres-Fuentes M Verónica Aguirre-Arias Renan Orellana Néstor Sepúlveda Ricardo D Moreno |
| author_facet | Julio Bunay Luz-Maria Gallardo Jorge Luis Torres-Fuentes M Verónica Aguirre-Arias Renan Orellana Néstor Sepúlveda Ricardo D Moreno |
| author_sort | Julio Bunay |
| collection | DOAJ |
| description | Obesity is a major worldwide health problem that is related to most chronic diseases, including male infertility. Owing to its wide impact on health, mechanisms underlying obesity-related infertility remain unknown. In this study, we report that mice fed a high-fat diet (HFD) for over 2 months showed reduced fertility rates and increased germ cell apoptosis, seminiferous tubule degeneration, and decreased intratesticular estradiol (E2) and E2-to-testosterone ratio. Interestingly, we also detected a decrease in testicular fatty acid levels, behenic acid (C22:0), and docosahexaenoic acid (DHA, 22:6n-3), which may be related to the production of dysfunctional spermatozoa. Overall, we did not detect any changes in the frequency of seminiferous tubule stages, sperm count, or rate of in vitro capacitation. However, there was an increase in spontaneous and progesterone-induced acrosomal exocytosis (acrosome reaction) in spermatozoa from HFD-fed mice. These data suggest that a decrease in E2 and fatty acid levels influences spermatogenesis and some steps of acrosome biogenesis that will have consequences for fertilization. Thus, our results add new evidence about the adverse effect of obesity in male reproduction and suggest that the acrosomal reaction can also be affected under this condition. |
| first_indexed | 2024-12-22T11:32:23Z |
| format | Article |
| id | doaj.art-590dd0e8c77543ada155013db01ba037 |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-12-22T11:32:23Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-590dd0e8c77543ada155013db01ba0372022-12-21T18:27:33ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622021-01-0123330631310.4103/aja.aja_76_20A decrease of docosahexaenoic acid in testes of mice fed a high-fat diet is associated with impaired sperm acrosome reaction and fertilityJulio BunayLuz-Maria GallardoJorge Luis Torres-FuentesM Verónica Aguirre-AriasRenan OrellanaNéstor SepúlvedaRicardo D MorenoObesity is a major worldwide health problem that is related to most chronic diseases, including male infertility. Owing to its wide impact on health, mechanisms underlying obesity-related infertility remain unknown. In this study, we report that mice fed a high-fat diet (HFD) for over 2 months showed reduced fertility rates and increased germ cell apoptosis, seminiferous tubule degeneration, and decreased intratesticular estradiol (E2) and E2-to-testosterone ratio. Interestingly, we also detected a decrease in testicular fatty acid levels, behenic acid (C22:0), and docosahexaenoic acid (DHA, 22:6n-3), which may be related to the production of dysfunctional spermatozoa. Overall, we did not detect any changes in the frequency of seminiferous tubule stages, sperm count, or rate of in vitro capacitation. However, there was an increase in spontaneous and progesterone-induced acrosomal exocytosis (acrosome reaction) in spermatozoa from HFD-fed mice. These data suggest that a decrease in E2 and fatty acid levels influences spermatogenesis and some steps of acrosome biogenesis that will have consequences for fertilization. Thus, our results add new evidence about the adverse effect of obesity in male reproduction and suggest that the acrosomal reaction can also be affected under this condition.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2021;volume=23;issue=3;spage=306;epage=313;aulast=Bunaycholesterol; estradiol; fat acid; testis; testosterone |
| spellingShingle | Julio Bunay Luz-Maria Gallardo Jorge Luis Torres-Fuentes M Verónica Aguirre-Arias Renan Orellana Néstor Sepúlveda Ricardo D Moreno A decrease of docosahexaenoic acid in testes of mice fed a high-fat diet is associated with impaired sperm acrosome reaction and fertility Asian Journal of Andrology cholesterol; estradiol; fat acid; testis; testosterone |
| title | A decrease of docosahexaenoic acid in testes of mice fed a high-fat diet is associated with impaired sperm acrosome reaction and fertility |
| title_full | A decrease of docosahexaenoic acid in testes of mice fed a high-fat diet is associated with impaired sperm acrosome reaction and fertility |
| title_fullStr | A decrease of docosahexaenoic acid in testes of mice fed a high-fat diet is associated with impaired sperm acrosome reaction and fertility |
| title_full_unstemmed | A decrease of docosahexaenoic acid in testes of mice fed a high-fat diet is associated with impaired sperm acrosome reaction and fertility |
| title_short | A decrease of docosahexaenoic acid in testes of mice fed a high-fat diet is associated with impaired sperm acrosome reaction and fertility |
| title_sort | decrease of docosahexaenoic acid in testes of mice fed a high fat diet is associated with impaired sperm acrosome reaction and fertility |
| topic | cholesterol; estradiol; fat acid; testis; testosterone |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2021;volume=23;issue=3;spage=306;epage=313;aulast=Bunay |
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