A Modified Protocol with Improved Detection Rate for Mis-Matched Donor HLA from Low Quantities of DNA in Urine Samples from Kidney Graft Recipients.

A Modified Protocol with Improved Detection Rate for Mis-Matched Donor HLA from Low Quantities of DNA in Urine Samples from Kidney Graft Recipients.

Urine from kidney transplant recipient has proven to be a viable source for donor DNA. However, an optimized protocol would be required to determine mis-matched donor HLA specificities in view of the scarcity of DNA obtained in some cases.In this study, fresh early morning urine specimens were obtai...

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Bibliographic Details
Main Authors: Janette Kwok, Leo C W Choi, Jenny C Y Ho, Gavin S W Chan, Maggie M Y Mok, Man-Fei Lam, Wai-Leung Chak, Au Cheuk, Ka-Foon Chau, Matthew Tong, Kwok-Wah Chan, Tak-Mao Chan
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5115744?pdf=render

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